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The map label for this gene is cspR [C]

Identifier: 123966176

GI number: 123966176

Start: 836702

End: 837184

Strand: Reverse

Name: cspR [C]

Synonym: P9515_09431

Alternate gene names: 123966176

Gene position: 837184-836702 (Counterclockwise)

Preceding gene: 123966177

Following gene: 123966174

Centisome position: 49.13

GC content: 27.33

Gene sequence:

>483_bases
TTGGAAATCTCACTTTTTGAACCGCGGATTCCTCAAAATACTGGTAGTATCGCTAGAACGTGTGCTGCTTTTGACATAAC
ATTAAATCTTATAGAGCCATTAGGTTTTAAATTAGAAGATAAATATTTAAAAAGAGCTGGTCTTGATTACTGGCCTTTAG
TTAAATTAAATAATTATCCTAATTTTAAAAAATTTAAGGAATCAAAAAAAAATAAAAGAATTATCGCCTTTAGCAAAAAA
AATGGTATTTATCTGAACAGATTTAGATTCTTAAAAGATGATATTTTATTATTTGGAAGAGAAGATACAGGATTACCAGA
AAGCGTTATTAACGGTAGTAATTCATTAATATCAATTTTCATGCCTAATGTAGAATTATCTAATAATGATAAAAAAGGTG
TTAGGAGTCTTAATTTATCAGTTGCGTGCGGAATAGCAATTTATGAGGCTAATAAACAAATAAATTTTCAAACTGGTAAT
TAA

Upstream 100 bases:

>100_bases
TCAAAAAAGAATAACTAGAATTTCAGAAGAGAATTGGCTAACCATAAATGGTAGAGCAATAAAATTAGATAAAATTAGTA
TTGAAATACCTTTTTAGTAA

Downstream 100 bases:

>100_bases
GACTATTCATGATTTAAGATATATGTAGGTCTCGGTAGCTCAGCTGGTTAGAGCGGCGGATTCATAGCCCGCAGGTCGCG
TGTTCAAGTCACGCTCGAGA

Product: SpoU family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MEISLFEPRIPQNTGSIARTCAAFDITLNLIEPLGFKLEDKYLKRAGLDYWPLVKLNNYPNFKKFKESKKNKRIIAFSKK
NGIYLNRFRFLKDDILLFGREDTGLPESVINGSNSLISIFMPNVELSNNDKKGVRSLNLSVACGIAIYEANKQINFQTGN

Sequences:

>Translated_160_residues
MEISLFEPRIPQNTGSIARTCAAFDITLNLIEPLGFKLEDKYLKRAGLDYWPLVKLNNYPNFKKFKESKKNKRIIAFSKK
NGIYLNRFRFLKDDILLFGREDTGLPESVINGSNSLISIFMPNVELSNNDKKGVRSLNLSVACGIAIYEANKQINFQTGN
>Mature_160_residues
MEISLFEPRIPQNTGSIARTCAAFDITLNLIEPLGFKLEDKYLKRAGLDYWPLVKLNNYPNFKKFKESKKNKRIIAFSKK
NGIYLNRFRFLKDDILLFGREDTGLPESVINGSNSLISIFMPNVELSNNDKKGVRSLNLSVACGIAIYEANKQINFQTGN

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=35.8974358974359, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18212; Mature: 18212

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISLFEPRIPQNTGSIARTCAAFDITLNLIEPLGFKLEDKYLKRAGLDYWPLVKLNNYP
CCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCC
NFKKFKESKKNKRIIAFSKKNGIYLNRFRFLKDDILLFGREDTGLPESVINGSNSLISIF
CHHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCEEEEECCCCCCCHHHHCCCCCEEEEE
MPNVELSNNDKKGVRSLNLSVACGIAIYEANKQINFQTGN
ECCEEECCCCCCCCEEECEEEEEEEEEEECCCEEEEEECC
>Mature Secondary Structure
MEISLFEPRIPQNTGSIARTCAAFDITLNLIEPLGFKLEDKYLKRAGLDYWPLVKLNNYP
CCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCC
NFKKFKESKKNKRIIAFSKKNGIYLNRFRFLKDDILLFGREDTGLPESVINGSNSLISIF
CHHHHHHCCCCCEEEEEECCCCEEEEEEEEECCCEEEEECCCCCCCHHHHCCCCCEEEEE
MPNVELSNNDKKGVRSLNLSVACGIAIYEANKQINFQTGN
ECCEEECCCCCCCCEEECEEEEEEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]