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The map label for this gene is sir [H]

Identifier: 123966060

GI number: 123966060

Start: 746528

End: 748318

Strand: Direct

Name: sir [H]

Synonym: P9515_08251

Alternate gene names: 123966060

Gene position: 746528-748318 (Clockwise)

Preceding gene: 123966058

Following gene: 123966071

Centisome position: 43.81

GC content: 33.39

Gene sequence:

>1791_bases
TTGATCAAGGTCGAAAAAGTAAAAAAGAAAAAAAAGATTGAAAAACAAGAGACGGTTTGCTTAGCAAATGGTCTAGAGGT
ATCTAAGTTTGAAAATTTCAAGAAGGGCAGTGAATTTCTCAAGGAACCACTTGCAAGCGAACTAAAGAATGAAAGTGATC
ATTTTACAAATGATGCTGTTCAACTATTGAAATTTCATGGAAGTTATCAACAAGATAATAGAGAAAACAGAAAGCCTGGC
AAAGGGAAAGATTGGCAGATGATGCTTAGGCTAAGAAGTCCTGGGGGAGAAATCCCAGGAAAACTATTTCTATCTTTAGA
TGAATTATCAGACAAACTCGGGAACGGCACACTGAGAATAACAACTAGACAAGCCTTTCAAATGCATGGAATCAGGAAAG
ATAATTTGAAGAAAGTTATTAAATCTATTGTAAATTCAATGGGCTCAACTTTAGCTGCGTGTGGGGACATAAATAGAAAT
GTCATGGCTCCTGCCGCACCTTTTGAAACATCAGAATACATAACTGCAAGAACTTTAGCCGTAAAAGTTGCCGATCTCTT
AACACCTGTAGCAGGACAAGGAACTTTTTTAGAGCTTTGGGCTGACGGAGATTTGGAATACACTATAAAACCAGATAAAG
AGATTGAAGAGAATAGAAAATTACAGTTTAAAGAGAATGTTTTTAGCGGGTCCAAAAAGGAACCTTTATATGGATCTACC
TACCTCCCAAGGAAGTTTAAATGTGCTGTCACCGTTCCTGGCGATAATTCTGTTGATCTTCTTACTAATGATATTGGAAT
AGTTGCATTCACTTCTAAAGAAGGGGAATTCGAAGGATGTAATTTTTATATAGGTGGAGGTATGGGTCGAACTCATAATA
ATGAAGAGACCTTTGCAAGAATTGCAGATCCACTTGGTTATGTTGATAAGAAAGATATATATGAACTAATTCAAAGTATC
GTTGCCATTCAAAGAGATTATGGAGATAGAAAATCAAGGAAAAATTCTAGAATGAAATACCTTTTGCATAGAAAAGGTAT
TAAATGGTTTAAAAAAATTCTTTTAGATAAATATTTTAGAAAAGAAATTAAGCCTTTAAGAAAAGAACCAGAGAATAAAT
TAATTGATTATCTAGGTTGGCATAAACAAAATGAAGATTATTATTTTGTTGGTTTACCTTTATTATCGGGAAGGCTTTAT
GGAAAGAAGAAATCACTAATAAGAAATATTGTTAAAGAAAATAATCTTGATTTAAGACTTACACCTAATCAAGATATTTT
ACTTTGTAATATTCCCAAAAAAAACAAAAGAGAAATTCAAAAAGCCTTAAAAAGTATTGGATATGACAATCTTCATGATA
TCAATGAAATTCAAAGACACGCTTTAGCATGTCCAGCCCTCCCTCTTTGTGGTCTGGCCATGACCGAAGCCGAAAGAATT
TTGCCTGAAGTTCTCAGTAGAATTGAAAATTTATTAAATGATATGAATATAGAAAAGACGATATTGTTCCGCATGACAGG
ATGCCCAAACGGATGTACAAGACCTTATATGGCAGAACTGGCTCTTGTAGGGAGTGGGCAAAATAAATATCAACTATGGT
TAGGCGGCAGTAAAAACCTTCAAAGGTTAGCGAGACCGTACTTGCAAAGAATGGATCTCGATGATTTAGAAAAAACTATT
AAACCATTACTTGAATTTTGGAAGAAAAGCAATCCAGAAAAAGATTTTGGTGATTTTATAAATAATGAAACTGATTCTTC
TATTAATAATTTATTAATAGAATTTGATTAA

Upstream 100 bases:

>100_bases
ATATTTAGACAAAAATAATTTATGGGCTATTACAACTATAATTTACTACCGGTTAAATAAGCTTTTCGCTAATGTATAAA
AAGAGATATGTTTTGGTCCC

Downstream 100 bases:

>100_bases
TATTTAAATTATCCCATATAAAGCATTAGCTTTTTTATTCTTCCAAGCCCATAATTGATCTCCATAGCTAAAATGCCACC
ATTCATTTGGATGTTGTACG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MIKVEKVKKKKKIEKQETVCLANGLEVSKFENFKKGSEFLKEPLASELKNESDHFTNDAVQLLKFHGSYQQDNRENRKPG
KGKDWQMMLRLRSPGGEIPGKLFLSLDELSDKLGNGTLRITTRQAFQMHGIRKDNLKKVIKSIVNSMGSTLAACGDINRN
VMAPAAPFETSEYITARTLAVKVADLLTPVAGQGTFLELWADGDLEYTIKPDKEIEENRKLQFKENVFSGSKKEPLYGST
YLPRKFKCAVTVPGDNSVDLLTNDIGIVAFTSKEGEFEGCNFYIGGGMGRTHNNEETFARIADPLGYVDKKDIYELIQSI
VAIQRDYGDRKSRKNSRMKYLLHRKGIKWFKKILLDKYFRKEIKPLRKEPENKLIDYLGWHKQNEDYYFVGLPLLSGRLY
GKKKSLIRNIVKENNLDLRLTPNQDILLCNIPKKNKREIQKALKSIGYDNLHDINEIQRHALACPALPLCGLAMTEAERI
LPEVLSRIENLLNDMNIEKTILFRMTGCPNGCTRPYMAELALVGSGQNKYQLWLGGSKNLQRLARPYLQRMDLDDLEKTI
KPLLEFWKKSNPEKDFGDFINNETDSSINNLLIEFD

Sequences:

>Translated_596_residues
MIKVEKVKKKKKIEKQETVCLANGLEVSKFENFKKGSEFLKEPLASELKNESDHFTNDAVQLLKFHGSYQQDNRENRKPG
KGKDWQMMLRLRSPGGEIPGKLFLSLDELSDKLGNGTLRITTRQAFQMHGIRKDNLKKVIKSIVNSMGSTLAACGDINRN
VMAPAAPFETSEYITARTLAVKVADLLTPVAGQGTFLELWADGDLEYTIKPDKEIEENRKLQFKENVFSGSKKEPLYGST
YLPRKFKCAVTVPGDNSVDLLTNDIGIVAFTSKEGEFEGCNFYIGGGMGRTHNNEETFARIADPLGYVDKKDIYELIQSI
VAIQRDYGDRKSRKNSRMKYLLHRKGIKWFKKILLDKYFRKEIKPLRKEPENKLIDYLGWHKQNEDYYFVGLPLLSGRLY
GKKKSLIRNIVKENNLDLRLTPNQDILLCNIPKKNKREIQKALKSIGYDNLHDINEIQRHALACPALPLCGLAMTEAERI
LPEVLSRIENLLNDMNIEKTILFRMTGCPNGCTRPYMAELALVGSGQNKYQLWLGGSKNLQRLARPYLQRMDLDDLEKTI
KPLLEFWKKSNPEKDFGDFINNETDSSINNLLIEFD
>Mature_596_residues
MIKVEKVKKKKKIEKQETVCLANGLEVSKFENFKKGSEFLKEPLASELKNESDHFTNDAVQLLKFHGSYQQDNRENRKPG
KGKDWQMMLRLRSPGGEIPGKLFLSLDELSDKLGNGTLRITTRQAFQMHGIRKDNLKKVIKSIVNSMGSTLAACGDINRN
VMAPAAPFETSEYITARTLAVKVADLLTPVAGQGTFLELWADGDLEYTIKPDKEIEENRKLQFKENVFSGSKKEPLYGST
YLPRKFKCAVTVPGDNSVDLLTNDIGIVAFTSKEGEFEGCNFYIGGGMGRTHNNEETFARIADPLGYVDKKDIYELIQSI
VAIQRDYGDRKSRKNSRMKYLLHRKGIKWFKKILLDKYFRKEIKPLRKEPENKLIDYLGWHKQNEDYYFVGLPLLSGRLY
GKKKSLIRNIVKENNLDLRLTPNQDILLCNIPKKNKREIQKALKSIGYDNLHDINEIQRHALACPALPLCGLAMTEAERI
LPEVLSRIENLLNDMNIEKTILFRMTGCPNGCTRPYMAELALVGSGQNKYQLWLGGSKNLQRLARPYLQRMDLDDLEKTI
KPLLEFWKKSNPEKDFGDFINNETDSSINNLLIEFD

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=567, Percent_Identity=32.6278659611993, Blast_Score=298, Evalue=8e-82,
Organism=Saccharomyces cerevisiae, GI6322597, Length=571, Percent_Identity=37.8283712784588, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 68162; Mature: 68162

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVEKVKKKKKIEKQETVCLANGLEVSKFENFKKGSEFLKEPLASELKNESDHFTNDAV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
QLLKFHGSYQQDNRENRKPGKGKDWQMMLRLRSPGGEIPGKLFLSLDELSDKLGNGTLRI
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEEHHHHHHHHCCCEEEE
TTRQAFQMHGIRKDNLKKVIKSIVNSMGSTLAACGDINRNVMAPAAPFETSEYITARTLA
EEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
VKVADLLTPVAGQGTFLELWADGDLEYTIKPDKEIEENRKLQFKENVFSGSKKEPLYGST
HHHHHHHCCCCCCCCEEEEEECCCEEEEECCCHHHHHCCCEEEHHHHHCCCCCCCCCCCC
YLPRKFKCAVTVPGDNSVDLLTNDIGIVAFTSKEGEFEGCNFYIGGGMGRTHNNEETFAR
CCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHH
IADPLGYVDKKDIYELIQSIVAIQRDYGDRKSRKNSRMKYLLHRKGIKWFKKILLDKYFR
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEIKPLRKEPENKLIDYLGWHKQNEDYYFVGLPLLSGRLYGKKKSLIRNIVKENNLDLRL
HHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCEEEE
TPNQDILLCNIPKKNKREIQKALKSIGYDNLHDINEIQRHALACPALPLCGLAMTEAERI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LPEVLSRIENLLNDMNIEKTILFRMTGCPNGCTRPYMAELALVGSGQNKYQLWLGGSKNL
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHEEEEECCCCEEEEEECCCHHH
QRLARPYLQRMDLDDLEKTIKPLLEFWKKSNPEKDFGDFINNETDSSINNLLIEFD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHCEEEEEC
>Mature Secondary Structure
MIKVEKVKKKKKIEKQETVCLANGLEVSKFENFKKGSEFLKEPLASELKNESDHFTNDAV
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
QLLKFHGSYQQDNRENRKPGKGKDWQMMLRLRSPGGEIPGKLFLSLDELSDKLGNGTLRI
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEEHHHHHHHHCCCEEEE
TTRQAFQMHGIRKDNLKKVIKSIVNSMGSTLAACGDINRNVMAPAAPFETSEYITARTLA
EEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
VKVADLLTPVAGQGTFLELWADGDLEYTIKPDKEIEENRKLQFKENVFSGSKKEPLYGST
HHHHHHHCCCCCCCCEEEEEECCCEEEEECCCHHHHHCCCEEEHHHHHCCCCCCCCCCCC
YLPRKFKCAVTVPGDNSVDLLTNDIGIVAFTSKEGEFEGCNFYIGGGMGRTHNNEETFAR
CCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHH
IADPLGYVDKKDIYELIQSIVAIQRDYGDRKSRKNSRMKYLLHRKGIKWFKKILLDKYFR
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEIKPLRKEPENKLIDYLGWHKQNEDYYFVGLPLLSGRLYGKKKSLIRNIVKENNLDLRL
HHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECHHHCCCCCCHHHHHHHHHHHCCCCEEEE
TPNQDILLCNIPKKNKREIQKALKSIGYDNLHDINEIQRHALACPALPLCGLAMTEAERI
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LPEVLSRIENLLNDMNIEKTILFRMTGCPNGCTRPYMAELALVGSGQNKYQLWLGGSKNL
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHEEEEECCCCEEEEEECCCHHH
QRLARPYLQRMDLDDLEKTIKPLLEFWKKSNPEKDFGDFINNETDSSINNLLIEFD
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8347657 [H]