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The map label for this gene is ilvD

Identifier: 123966044

GI number: 123966044

Start: 728392

End: 730071

Strand: Direct

Name: ilvD

Synonym: P9515_08091

Alternate gene names: 123966044

Gene position: 728392-730071 (Clockwise)

Preceding gene: 123966043

Following gene: 123966052

Centisome position: 42.74

GC content: 35.77

Gene sequence:

>1680_bases
ATGAATAAATTAAGATCTTCTGCAATAACTCAAGGTGTTCAAAGATCTCCTAACAGATCAATGTTAAGAGCAGTTGGATT
TAGTGATGATGATTTCACTAAACCAATCATTGGAGTCGCTAATGGATATAGCACCATTACACCCTGCAATATGGGCCTTA
ACAAGTTAGCTCTTAAGGCAGAAGATTCAATAAGGAAATCAGGAGGAATGCCTCAAATGTTCGGAACTATCACCGTAAGC
GATGGGATCTCCATGGGAACAGAAGGAATGAAATATTCTTTAGTTTCAAGGGAAGTAATAGCCGATTCAATAGAAACTGC
CTGTAATGCGCAAAGTATGGATGGTGTTTTAGCTATTGGTGGTTGTGATAAAAATATGCCGGGTGCAATGATAGCAATTG
CAAGAATGAATATCCCTTCCATATTTATTTATGGAGGAACAATAAAACCTGGAAAGTTAAACGGTGAAGACTTAACGGTT
GTCAGTGCATTTGAAGCTGTTGGGCAATTAACCTCGGGTAAAATAAATGAGAAGAGATTAAATGAAGTTGAGAAAAATTG
TATTCCAGGAGCAGGAAGTTGTGGAGGAATGTTCACAGCTAATACTATGTCAGCAGTTATAGAAGTCTTAGGTTTAAGTC
TTCCTCATAGTTCAACAATGGCAGCCGAAGATTATGAGAAGGTGGTAAGTGCTGAAAAAAGCGCTGAAATACTTGTAGAT
GCAATAAAAAAAGATATAAGGCCACTAGATCTTATGACTAAAGAATCTTTTGAAAATGCAATATCTGTAATTATGGCTAT
TGGAGGTTCGACAAATGCAGTGCTTCACATATTAGCGATAGCGAATACTGCAGGTATAGAGATCAATATTGATGATTTTG
AAAGAATAAGACAAAAAGTTCCTGTGATTTGTGATTTGAAACCTAGCGGCAGATATGTCACAGTAGATCTTCATAATGCA
GGAGGCATTCCACAAGTAATGAAAATACTTTTAAATGCAGGATTAATAAATGGTGATTGCAAAAATATCGAAGGTAAAAC
AATATCTGAATATTTACTTAATATTCCAGATAATCCTCCTGAAAATCAAAATGTTATTAGAAACATAAATAATCCTCTTT
ATAAAAAAGGTCATTTAGCAATATTAAAAGGTAATTTAGCTTCGGAAGGTTGTGTAGCAAAAATCAGCGGAGTAAAGAAT
CCAGTTTTAAAGGGTCCCGCTCGAATTTTTGAAAGTGAGGAAGATTGTCTTAAATCAATTTTGAATAATGATATTAAAGC
TGGTAATGTTGTCGTTATAAGAAATGAAGGTCCTGTGGGAGGACCGGGAATGAGAGAAATGTTAGCTCCAACATCTGCAA
TTGTTGGGCAAGGGCTGGGAGAAAAAGTAGCTTTAATAACTGATGGTAGATTTAGTGGAGGTACCTATGGCCTTGTTGTT
GGACATATCGCACCAGAGGCAGCAGTAGGAGGGAATATAGCTTTAATAAGAGAGGGAGATTTAATCACTGTTGATGCTGT
AAACCAATTAATTGAAGTTGAACTATCTGATGGGGAATTAGAAAAAAGACGAATTAACTGGATAAAGCCTATTCCGAAAT
ATAAGAAAGGTGTACTTTCAAAATATTCAAGAATTGTAAGTACTTCAAGCTTAGGTGCAGTTACAGATCTGGAAAAATAA

Upstream 100 bases:

>100_bases
AGTTAGAACAAAAAGTTGAACAACTAAATTCTGCAATTGCTGATGTAAGGAGACAACTTGAGGAGTTAGGACAATAAAAT
AAAACAGGTTCTCAAATCAT

Downstream 100 bases:

>100_bases
TATCCAAAAAATAAATTTTTTTAAGTAATAAAATTTTTTATTCTAGTAGCAAGATTCTCCAATTTTGAACTATATTTTGG
AGGTAAACATTTCTTCCCAC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFSDDDFTKPIIGVANGYSTITPCNMGLNKLALKAEDSIRKSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLNGEDLTV
VSAFEAVGQLTSGKINEKRLNEVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDYEKVVSAEKSAEILVD
AIKKDIRPLDLMTKESFENAISVIMAIGGSTNAVLHILAIANTAGIEINIDDFERIRQKVPVICDLKPSGRYVTVDLHNA
GGIPQVMKILLNAGLINGDCKNIEGKTISEYLLNIPDNPPENQNVIRNINNPLYKKGHLAILKGNLASEGCVAKISGVKN
PVLKGPARIFESEEDCLKSILNNDIKAGNVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIREGDLITVDAVNQLIEVELSDGELEKRRINWIKPIPKYKKGVLSKYSRIVSTSSLGAVTDLEK

Sequences:

>Translated_559_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFSDDDFTKPIIGVANGYSTITPCNMGLNKLALKAEDSIRKSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLNGEDLTV
VSAFEAVGQLTSGKINEKRLNEVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDYEKVVSAEKSAEILVD
AIKKDIRPLDLMTKESFENAISVIMAIGGSTNAVLHILAIANTAGIEINIDDFERIRQKVPVICDLKPSGRYVTVDLHNA
GGIPQVMKILLNAGLINGDCKNIEGKTISEYLLNIPDNPPENQNVIRNINNPLYKKGHLAILKGNLASEGCVAKISGVKN
PVLKGPARIFESEEDCLKSILNNDIKAGNVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIREGDLITVDAVNQLIEVELSDGELEKRRINWIKPIPKYKKGVLSKYSRIVSTSSLGAVTDLEK
>Mature_559_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFSDDDFTKPIIGVANGYSTITPCNMGLNKLALKAEDSIRKSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLNGEDLTV
VSAFEAVGQLTSGKINEKRLNEVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDYEKVVSAEKSAEILVD
AIKKDIRPLDLMTKESFENAISVIMAIGGSTNAVLHILAIANTAGIEINIDDFERIRQKVPVICDLKPSGRYVTVDLHNA
GGIPQVMKILLNAGLINGDCKNIEGKTISEYLLNIPDNPPENQNVIRNINNPLYKKGHLAILKGNLASEGCVAKISGVKN
PVLKGPARIFESEEDCLKSILNNDIKAGNVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIREGDLITVDAVNQLIEVELSDGELEKRRINWIKPIPKYKKGVLSKYSRIVSTSSLGAVTDLEK

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=613, Percent_Identity=38.3360522022839, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1788157, Length=475, Percent_Identity=31.1578947368421, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI2367371, Length=481, Percent_Identity=32.8482328482328, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786464, Length=509, Percent_Identity=31.6306483300589, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6322476, Length=564, Percent_Identity=46.6312056737589, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_PROM5 (A2BW57)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011125.1
- ProteinModelPortal:   A2BW57
- SMR:   A2BW57
- STRING:   A2BW57
- GeneID:   4719391
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_08091
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- ProtClustDB:   PRK00911
- BioCyc:   PMAR167542:P9515ORF_0850-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59305; Mature: 59305

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLRSSAITQGVQRSPNRSMLRAVGFSDDDFTKPIIGVANGYSTITPCNMGLNKLALKA
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHEEECC
EDSIRKSGGMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIG
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLNGEDLTVVSAFEAVGQLTSGKINEKRL
CCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
NEVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDYEKVVSAEKSAEILVD
HHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
AIKKDIRPLDLMTKESFENAISVIMAIGGSTNAVLHILAIANTAGIEINIDDFERIRQKV
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEECCCCEEEEHHHHHHHHHHC
PVICDLKPSGRYVTVDLHNAGGIPQVMKILLNAGLINGDCKNIEGKTISEYLLNIPDNPP
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCC
ENQNVIRNINNPLYKKGHLAILKGNLASEGCVAKISGVKNPVLKGPARIFESEEDCLKSI
CCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCHHCCHHHHHCCHHHHHHHH
LNNDIKAGNVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
HCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
GHIAPEAAVGGNIALIREGDLITVDAVNQLIEVELSDGELEKRRINWIKPIPKYKKGVLS
ECCCCCHHCCCCEEEEECCCEEEHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHH
KYSRIVSTSSLGAVTDLEK
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNKLRSSAITQGVQRSPNRSMLRAVGFSDDDFTKPIIGVANGYSTITPCNMGLNKLALKA
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHEEECC
EDSIRKSGGMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIG
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLNGEDLTVVSAFEAVGQLTSGKINEKRL
CCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
NEVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDYEKVVSAEKSAEILVD
HHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
AIKKDIRPLDLMTKESFENAISVIMAIGGSTNAVLHILAIANTAGIEINIDDFERIRQKV
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEECCCCEEEEHHHHHHHHHHC
PVICDLKPSGRYVTVDLHNAGGIPQVMKILLNAGLINGDCKNIEGKTISEYLLNIPDNPP
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCC
ENQNVIRNINNPLYKKGHLAILKGNLASEGCVAKISGVKNPVLKGPARIFESEEDCLKSI
CCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCHHCCHHHHHCCHHHHHHHH
LNNDIKAGNVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
HCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
GHIAPEAAVGGNIALIREGDLITVDAVNQLIEVELSDGELEKRRINWIKPIPKYKKGVLS
ECCCCCHHCCCCEEEEECCCEEEHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHH
KYSRIVSTSSLGAVTDLEK
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA