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The map label for this gene is proA

Identifier: 123965890

GI number: 123965890

Start: 589282

End: 590592

Strand: Direct

Name: proA

Synonym: P9515_06551

Alternate gene names: 123965890

Gene position: 589282-590592 (Clockwise)

Preceding gene: 123965889

Following gene: 123965891

Centisome position: 34.58

GC content: 33.18

Gene sequence:

>1311_bases
ATGACTGATATATTTGAAGTTCCCAGCCCAGATAATAATCTTTTAGATAAAGCTGAGCAACTTCGCTTAGCTTCAATTAA
AACTAGTCAAACAAATAATAATGAGAGAATTAGAGCTTTGAATTTAATGGCTGACTCTCTAGAAAAAAACTCAAAAGAAA
TAATTGACTCTAATTTTGAAGATTATAAAAAAGCAGAAATAAAAGGAATTTCAAAGGCCTTACTTTCTAGATTAAAGCTA
TCAAAAGAAAAACTAAATTTAGGAATAGAAGGGATAAGAAAAGTTGGTGACTTGTCAGATCCACTAGGTCAAATCCAAAT
AAAAAAGGAACTTTCCAAAGGACTCATTCTAGAGAGAAAAACAGTTCCTATTGGAGTGCTAGGAGTGATTTTCGAATCAA
GACCTGATGCAGTAATGCAAATAAGTTCTTTGGCAATTAGAGCTGGGAATGGAGTAATGCTTAAAGGGGGGAGTGAAGCC
AATTTTACTAATCAAGCAATTGTTAGTGCACTTAAGAAGGGTTTGCAGAAATCAAATATCGATGATAACGCTATCTGTCT
TTTAACAAGTAGAAAAGATAGTATGGCGATGCTGAACTTAGAAAAATTTATAAATTTAATTATTCCAAGAGGAAGTAATG
AACTCGTTAAATTTATACAGGAAAATACAGGAATCCCTGTTCTTGGCCATGCTGATGGGATTTGTCATTTATACATAGAC
GATGAAGTAAATCTTGATATTGCTTTAAAAGTGGCTTTAGATAGTAAGATTCAATATCCAGCTGCCTGTAATGCTATTGA
GACTCTTTTAATACATAAAAGTATTGCCCCTGCCTTTCTGAAAAAAGCTATCCCAATTTTTAATTCTAATAATGTTAAGT
TGATTGGAGATAAAAAAGCTGTTAAATTGGGCGTCGCTTTTGAAGCTAATTACGAGGACTGGCAAACTGAATATTTAGAT
CTTATTCTCTCTATAAAAATTGTTGATGATTTAGAAGAAGGAATTGCTCATATACAAAAATTCAGTTCAAAACATACAGA
TGGAATAATCACTGAAAATATAAGTAACGCTAATAAATTCATGAGTGAGATAGATAGTGCAGGGGTTTTTCATAATTGCT
CAACAAGGTTCGCAGATGGTTTTAGATATGGCTTTGGAGCTGAAGTTGGGATATCTACTCAGACATTACCCCCAAGAGGT
CCAGTAGGACTGGAGGGGTTGGTAACGTATAAGTATTTTTTAAGAGGTGAAGGCCATAGTGTTGATGATTTTTCATCTGG
AAAATCAATATATACCCATAAAGATCTTTAA

Upstream 100 bases:

>100_bases
GAAAAGTCAACTACAGTTATTTTTTAGATATTAATTCCTAAAATAAAAGTTTTAAGAATGCTTATTTTGACTTATTATTA
AATTTAAATAAAATCAAATT

Downstream 100 bases:

>100_bases
ATATTATAAGAATGGGAACATATTTAAAAATTAAGAATATTAAACTTTGGTCAAGAGTTGGTGTCCTAGAAGAAGAAAGA
GAATTAGGGCAACTTTTTAG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTDIFEVPSPDNNLLDKAEQLRLASIKTSQTNNNERIRALNLMADSLEKNSKEIIDSNFEDYKKAEIKGISKALLSRLKL
SKEKLNLGIEGIRKVGDLSDPLGQIQIKKELSKGLILERKTVPIGVLGVIFESRPDAVMQISSLAIRAGNGVMLKGGSEA
NFTNQAIVSALKKGLQKSNIDDNAICLLTSRKDSMAMLNLEKFINLIIPRGSNELVKFIQENTGIPVLGHADGICHLYID
DEVNLDIALKVALDSKIQYPAACNAIETLLIHKSIAPAFLKKAIPIFNSNNVKLIGDKKAVKLGVAFEANYEDWQTEYLD
LILSIKIVDDLEEGIAHIQKFSSKHTDGIITENISNANKFMSEIDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRG
PVGLEGLVTYKYFLRGEGHSVDDFSSGKSIYTHKDL

Sequences:

>Translated_436_residues
MTDIFEVPSPDNNLLDKAEQLRLASIKTSQTNNNERIRALNLMADSLEKNSKEIIDSNFEDYKKAEIKGISKALLSRLKL
SKEKLNLGIEGIRKVGDLSDPLGQIQIKKELSKGLILERKTVPIGVLGVIFESRPDAVMQISSLAIRAGNGVMLKGGSEA
NFTNQAIVSALKKGLQKSNIDDNAICLLTSRKDSMAMLNLEKFINLIIPRGSNELVKFIQENTGIPVLGHADGICHLYID
DEVNLDIALKVALDSKIQYPAACNAIETLLIHKSIAPAFLKKAIPIFNSNNVKLIGDKKAVKLGVAFEANYEDWQTEYLD
LILSIKIVDDLEEGIAHIQKFSSKHTDGIITENISNANKFMSEIDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRG
PVGLEGLVTYKYFLRGEGHSVDDFSSGKSIYTHKDL
>Mature_435_residues
TDIFEVPSPDNNLLDKAEQLRLASIKTSQTNNNERIRALNLMADSLEKNSKEIIDSNFEDYKKAEIKGISKALLSRLKLS
KEKLNLGIEGIRKVGDLSDPLGQIQIKKELSKGLILERKTVPIGVLGVIFESRPDAVMQISSLAIRAGNGVMLKGGSEAN
FTNQAIVSALKKGLQKSNIDDNAICLLTSRKDSMAMLNLEKFINLIIPRGSNELVKFIQENTGIPVLGHADGICHLYIDD
EVNLDIALKVALDSKIQYPAACNAIETLLIHKSIAPAFLKKAIPIFNSNNVKLIGDKKAVKLGVAFEANYEDWQTEYLDL
ILSIKIVDDLEEGIAHIQKFSSKHTDGIITENISNANKFMSEIDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGP
VGLEGLVTYKYFLRGEGHSVDDFSSGKSIYTHKDL

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=407, Percent_Identity=43.7346437346437, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI21361368, Length=407, Percent_Identity=43.7346437346437, Blast_Score=327, Evalue=2e-89,
Organism=Escherichia coli, GI1786438, Length=403, Percent_Identity=38.7096774193548, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17569967, Length=423, Percent_Identity=44.2080378250591, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17569969, Length=423, Percent_Identity=44.2080378250591, Blast_Score=347, Evalue=5e-96,
Organism=Saccharomyces cerevisiae, GI6324899, Length=428, Percent_Identity=41.1214953271028, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21357643, Length=424, Percent_Identity=41.9811320754717, Blast_Score=327, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_PROM5 (A2BVQ3)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010971.1
- ProteinModelPortal:   A2BVQ3
- SMR:   A2BVQ3
- STRING:   A2BVQ3
- GeneID:   4719664
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_06551
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   PMAR167542:P9515ORF_0691-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 47922; Mature: 47790

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIFEVPSPDNNLLDKAEQLRLASIKTSQTNNNERIRALNLMADSLEKNSKEIIDSNFE
CCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHCCCHHHHCCCHH
DYKKAEIKGISKALLSRLKLSKEKLNLGIEGIRKVGDLSDPLGQIQIKKELSKGLILERK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHEEEHHHHHCCCEEEEC
TVPIGVLGVIFESRPDAVMQISSLAIRAGNGVMLKGGSEANFTNQAIVSALKKGLQKSNI
CCCEEEEEEEECCCCHHHHHHHHHEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
DDNAICLLTSRKDSMAMLNLEKFINLIIPRGSNELVKFIQENTGIPVLGHADGICHLYID
CCCEEEEEECCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEECCCCEEEEEEC
DEVNLDIALKVALDSKIQYPAACNAIETLLIHKSIAPAFLKKAIPIFNSNNVKLIGDKKA
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCE
VKLGVAFEANYEDWQTEYLDLILSIKIVDDLEEGIAHIQKFSSKHTDGIITENISNANKF
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHH
MSEIDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGPVGLEGLVTYKYFLRGEGHS
HHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEEECCCCC
VDDFSSGKSIYTHKDL
CCCCCCCCEEEEECCC
>Mature Secondary Structure 
TDIFEVPSPDNNLLDKAEQLRLASIKTSQTNNNERIRALNLMADSLEKNSKEIIDSNFE
CCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHCCCHHHHCCCHH
DYKKAEIKGISKALLSRLKLSKEKLNLGIEGIRKVGDLSDPLGQIQIKKELSKGLILERK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHEEEHHHHHCCCEEEEC
TVPIGVLGVIFESRPDAVMQISSLAIRAGNGVMLKGGSEANFTNQAIVSALKKGLQKSNI
CCCEEEEEEEECCCCHHHHHHHHHEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
DDNAICLLTSRKDSMAMLNLEKFINLIIPRGSNELVKFIQENTGIPVLGHADGICHLYID
CCCEEEEEECCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCEEEECCCCEEEEEEC
DEVNLDIALKVALDSKIQYPAACNAIETLLIHKSIAPAFLKKAIPIFNSNNVKLIGDKKA
CCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCE
VKLGVAFEANYEDWQTEYLDLILSIKIVDDLEEGIAHIQKFSSKHTDGIITENISNANKF
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHH
MSEIDSAGVFHNCSTRFADGFRYGFGAEVGISTQTLPPRGPVGLEGLVTYKYFLRGEGHS
HHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEEECCCCC
VDDFSSGKSIYTHKDL
CCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA