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The map label for this gene is purA [H]

Identifier: 123965805

GI number: 123965805

Start: 508650

End: 509960

Strand: Reverse

Name: purA [H]

Synonym: P9515_05701

Alternate gene names: 123965805

Gene position: 509960-508650 (Counterclockwise)

Preceding gene: 123965806

Following gene: 123965804

Centisome position: 29.92

GC content: 36.69

Gene sequence:

>1311_bases
TTGGCTAATGTTGTTGTTATCGGAGCTCAATGGGGTGACGAAGGAAAAGGTAAAATAACCGATTTATTAAGTCGATCAGC
AGATGTTGTTGTTCGATATCAAGGAGGTGTGAATGCTGGGCACACCATTGTTGTCGATGATAAGGTATTAAAGTTACATT
TAATTCCCTCTGGAATACTTTATGAAGACACTACCTGTTTGATTGGATCAGGAACAGTAGTTGACCCAAAGATATTGCTT
AAAGAAATAGATATGTTAATTGAGAATGGAATAGATATTTCGGGACTAAAAATTTCATCTACTTCGCATGTAACAATGCC
TTACCATCGTTTATTAGATGAAGCTATGGAAGCAGATAGAGGTTCAAATAAAATAGGAACAACGGGGAGAGGAATTGGCC
CAACATATGCTGATAAATCACAGAGAAATGGGATTCGAATAAGAGACTTACTCAATGAAGATAGATTAAAAGATGTTTTA
GAGATCCCACTTAAAGAAAAAAATGGAGTACTTGAAAAGATATATGGCATTAGACCTCTTTGTAAAGATGAAATAATTAA
AGAATATCTAGATTATGGGAAGAGGTTATCTAAGCATGTAGTTGACTGTACGAGAACTATTCACTCAGCCGCGAAAAATA
AGCAAAACATTCTATTTGAAGGAGCACAGGGAACATTACTTGACTTAGATCATGGAACATATCCTTTTGTAACCTCATCG
AATCCTATATCTGGAGGAGCATGTATCGGAGCTGGAGTTGGGCCAACGCTTATAGATAGGGTTATAGGAGTGGCAAAGGC
ATATACTACTAGAGTTGGGGAGGGGCCATTCGTTACAGAATTACAAGGAAGTATTAATGATCAACTTTGCGATAGAGGAA
GTGAGTTTGGAACCACCACAGGAAGAAGAAGAAGATGTGGATGGTTTGATGGAGTTATTGGTAAGTATGCTGTTTATGTA
AATGGTCTTGACTGTTTAGCAGTAACAAAACTAGACGTCCTAGATGAGTTAGATGAAATTCAAGTCTGTATTGCCTATGA
ATTGGATGGAAAAGAAATAGATTATTTCCCCACAAATTCTGATGACTTAAAAAAATGCAAACCAATATTTAAAAAATTAA
AAGGCTGGCAATGTTCTACCGCTAACTGCCGAAAACTATCTGATCTGCCTGAAAATGCAATGAATTACCTAAGATTTCTT
GCTGAATTAATGGAAGTACCAATTGCTATCGTTTCATTAGGTGCCAATAGAGATCAAACTATAGTGATTGAAGATCCTAT
CCACGGTCCGAAGAGAGCTCTTCTAAGATAA

Upstream 100 bases:

>100_bases
GGTTCTCAGAGAAAGTTAATACAAATGATTTACTTTTTTAGAGTTATACAAGAATTTTGAATATATTATATATTCGTACA
AAAATAATGCTTTTTTAAAA

Downstream 100 bases:

>100_bases
TCAAAAAATTTTTAAATTAAATGAAAGAAAAAAATAAAAATTTTGATCAAAAAAAAGGTATTGATCTTATTGGTTTGGGA
AACGCAATCGTAGATATTAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYEDTTCLIGSGTVVDPKILL
KEIDMLIENGIDISGLKISSTSHVTMPYHRLLDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNEDRLKDVL
EIPLKEKNGVLEKIYGIRPLCKDEIIKEYLDYGKRLSKHVVDCTRTIHSAAKNKQNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFVTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVYV
NGLDCLAVTKLDVLDELDEIQVCIAYELDGKEIDYFPTNSDDLKKCKPIFKKLKGWQCSTANCRKLSDLPENAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Sequences:

>Translated_436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYEDTTCLIGSGTVVDPKILL
KEIDMLIENGIDISGLKISSTSHVTMPYHRLLDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNEDRLKDVL
EIPLKEKNGVLEKIYGIRPLCKDEIIKEYLDYGKRLSKHVVDCTRTIHSAAKNKQNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFVTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVYV
NGLDCLAVTKLDVLDELDEIQVCIAYELDGKEIDYFPTNSDDLKKCKPIFKKLKGWQCSTANCRKLSDLPENAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR
>Mature_435_residues
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYEDTTCLIGSGTVVDPKILLK
EIDMLIENGIDISGLKISSTSHVTMPYHRLLDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNEDRLKDVLE
IPLKEKNGVLEKIYGIRPLCKDEIIKEYLDYGKRLSKHVVDCTRTIHSAAKNKQNILFEGAQGTLLDLDHGTYPFVTSSN
PISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFVTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVYVN
GLDCLAVTKLDVLDELDEIQVCIAYELDGKEIDYFPTNSDDLKKCKPIFKKLKGWQCSTANCRKLSDLPENAMNYLRFLA
ELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=443, Percent_Identity=41.7607223476298, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=43.1924882629108, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI40316944, Length=412, Percent_Identity=40.7766990291262, Blast_Score=281, Evalue=6e-76,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=46.3700234192037, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=41.3953488372093, Blast_Score=307, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=41.3953488372093, Blast_Score=307, Evalue=9e-84,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=41.4187643020595, Blast_Score=325, Evalue=8e-90,
Organism=Drosophila melanogaster, GI21356033, Length=420, Percent_Identity=41.9047619047619, Blast_Score=329, Evalue=3e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47849; Mature: 47717

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCE
YEDTTCLIGSGTVVDPKILLKEIDMLIENGIDISGLKISSTSHVTMPYHRLLDEAMEADR
ECCCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNEDRLKDVLEIPLKEKNGVLEKIYGIRPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
CKDEIIKEYLDYGKRLSKHVVDCTRTIHSAAKNKQNILFEGAQGTLLDLDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFVTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVYVNGLDCLAVTKLDVLDELDEIQVCIAYELDGKEIDYFPTNS
CCCCCCCHHHHHHHCEEEEECCCCEEHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCC
DDLKKCKPIFKKLKGWQCSTANCRKLSDLPENAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC
>Mature Secondary Structure 
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCE
YEDTTCLIGSGTVVDPKILLKEIDMLIENGIDISGLKISSTSHVTMPYHRLLDEAMEADR
ECCCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNEDRLKDVLEIPLKEKNGVLEKIYGIRPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
CKDEIIKEYLDYGKRLSKHVVDCTRTIHSAAKNKQNILFEGAQGTLLDLDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFVTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVYVNGLDCLAVTKLDVLDELDEIQVCIAYELDGKEIDYFPTNS
CCCCCCCHHHHHHHCEEEEECCCCEEHHHHHHHHHHHCCEEEEEEEEECCCEEECCCCCC
DDLKKCKPIFKKLKGWQCSTANCRKLSDLPENAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA