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The map label for this gene is hemL [H]

Identifier: 123965782

GI number: 123965782

Start: 487048

End: 488289

Strand: Reverse

Name: hemL [H]

Synonym: P9515_05471

Alternate gene names: 123965782

Gene position: 488289-487048 (Counterclockwise)

Preceding gene: 123965783

Following gene: 123965776

Centisome position: 28.65

GC content: 40.5

Gene sequence:

>1242_bases
ATGCCTGGAGGGGTTAGTTCTCCGGTAAGAGCATTTAAGTCTGTTGGTGGACAGCCCATTGTTTTTGATAGAGTAAAAGG
CCCATTTGCCTGGGACGTTGATGGGAACAGGTATATCGATTACATAGGTAGTTGGGGACCAGCTATTTGTGGGCATGCTC
ACCCTGAAGTGATTACAGCATTGCAAGAGGCAATAGAAAAAGGCACTAGTTTTGGTGCTCCTTGTGTTCAAGAGAATAAA
CTTGCTGAAATGGTGATTGACGCTGTTCCTTCGGTAGAGATGGTCAGATTTGTCAACAGTGGCACAGAAGCTTGCATGGC
AGTTCTAAGGCTAATGAGGGCATTTACAGGAAGAGATAAAGTAATAAAGTTTGATGGTTGTTACCATGGCCATGCTGACA
TGTTTTTGGTAAAAGCTGGTTCTGGAGTAGCTACTTTAGGCCTTCCAGATTCCCCCGGCGTTCCAAGAACTACAACAGCA
AACACTCTAACTGCACCTTACAACGATCTTGAAGCTGTAAAAAAATTATTTTCGGAAAATCCTGATGCAATATCTGGAGT
CATTCTTGAACCTATAGTTGGTAATGCAGGATTTATTACCCCTGAACCTGGTTTCCTTGAAGGATTGAGAGAACTCACAA
CTGAAAATGGATCTTTACTAGTTTTTGATGAGGTAATGACTGGTTTCAGAATTAGCTATGGAGGAGCACAAGAAAAATTC
GGAGTTACGCCTGATTTAACGACCCTAGGCAAAGTCATTGGAGGAGGCTTACCTGTAGGAGCATATGGGGGAAGGAAAGA
AATAATGTCCATGGTTGCGCCATCAGGTCCTGTATATCAAGCAGGAACATTAAGTGGTAATCCATTAGCAATGACAGCAG
GTATCAAAACTCTTGAGCTACTTAAACAAGAAGGTACTTATGAAAAAATGGGGTTAGCTACCTCTAGATTAATTGAAGGT
ATAATGCAATCTGCTGAGAATAATGGTATCGCTATAAATGGTGGAAGCGTTAGTGCAATGTTCGGATTTTTCTTGTGCGA
AGGTCCAGTAAGAAACTTTGAAGAAGCTAAAACAAATAATTCAGAACTTTTTGGAAAACTACACAGGGAGATGATAAAAC
GAGGCGTATATTTAGCTCCAAGTCCATTTGAAGCTGGATTTACTTCATTAGCTCATAGTGAAGAAGAAATTGATCGAACC
ATAGAAGCATTCGATCAATCTTTTAGCGTTATAAAAAATTAA

Upstream 100 bases:

>100_bases
AACATGACGAAACTTTCTCAAAAATCTATTTATTTAAATTTTGACTGACATATTTAATATCACTCATTCTGAAGAAGTTT
TTTCTTCCGCTTTAAAATTG

Downstream 100 bases:

>100_bases
TTTTATCTATTATTTCCTCCAACTATTACAGGTGGAGTTCCTCCATTAGTTCCAGGAAGTCCGGGAACTACTTGTGTGCT
ACCGTCCCATTTATCAAGGA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPGGVSSPVRAFKSVGGQPIVFDRVKGPFAWDVDGNRYIDYIGSWGPAICGHAHPEVITALQEAIEKGTSFGAPCVQENK
LAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDKVIKFDGCYHGHADMFLVKAGSGVATLGLPDSPGVPRTTTA
NTLTAPYNDLEAVKKLFSENPDAISGVILEPIVGNAGFITPEPGFLEGLRELTTENGSLLVFDEVMTGFRISYGGAQEKF
GVTPDLTTLGKVIGGGLPVGAYGGRKEIMSMVAPSGPVYQAGTLSGNPLAMTAGIKTLELLKQEGTYEKMGLATSRLIEG
IMQSAENNGIAINGGSVSAMFGFFLCEGPVRNFEEAKTNNSELFGKLHREMIKRGVYLAPSPFEAGFTSLAHSEEEIDRT
IEAFDQSFSVIKN

Sequences:

>Translated_413_residues
MPGGVSSPVRAFKSVGGQPIVFDRVKGPFAWDVDGNRYIDYIGSWGPAICGHAHPEVITALQEAIEKGTSFGAPCVQENK
LAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDKVIKFDGCYHGHADMFLVKAGSGVATLGLPDSPGVPRTTTA
NTLTAPYNDLEAVKKLFSENPDAISGVILEPIVGNAGFITPEPGFLEGLRELTTENGSLLVFDEVMTGFRISYGGAQEKF
GVTPDLTTLGKVIGGGLPVGAYGGRKEIMSMVAPSGPVYQAGTLSGNPLAMTAGIKTLELLKQEGTYEKMGLATSRLIEG
IMQSAENNGIAINGGSVSAMFGFFLCEGPVRNFEEAKTNNSELFGKLHREMIKRGVYLAPSPFEAGFTSLAHSEEEIDRT
IEAFDQSFSVIKN
>Mature_412_residues
PGGVSSPVRAFKSVGGQPIVFDRVKGPFAWDVDGNRYIDYIGSWGPAICGHAHPEVITALQEAIEKGTSFGAPCVQENKL
AEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDKVIKFDGCYHGHADMFLVKAGSGVATLGLPDSPGVPRTTTAN
TLTAPYNDLEAVKKLFSENPDAISGVILEPIVGNAGFITPEPGFLEGLRELTTENGSLLVFDEVMTGFRISYGGAQEKFG
VTPDLTTLGKVIGGGLPVGAYGGRKEIMSMVAPSGPVYQAGTLSGNPLAMTAGIKTLELLKQEGTYEKMGLATSRLIEGI
MQSAENNGIAINGGSVSAMFGFFLCEGPVRNFEEAKTNNSELFGKLHREMIKRGVYLAPSPFEAGFTSLAHSEEEIDRTI
EAFDQSFSVIKN

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=307, Percent_Identity=31.2703583061889, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI13994255, Length=327, Percent_Identity=27.217125382263, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI37574042, Length=328, Percent_Identity=26.8292682926829, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI24119277, Length=318, Percent_Identity=27.3584905660377, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI226442705, Length=328, Percent_Identity=26.5243902439024, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI284507298, Length=152, Percent_Identity=37.5, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI226442709, Length=293, Percent_Identity=26.962457337884, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786349, Length=410, Percent_Identity=53.1707317073171, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1789016, Length=416, Percent_Identity=29.5673076923077, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1788044, Length=295, Percent_Identity=36.271186440678, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789759, Length=307, Percent_Identity=31.2703583061889, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693181, Length=305, Percent_Identity=30.8196721311475, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1787560, Length=309, Percent_Identity=29.126213592233, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786991, Length=278, Percent_Identity=26.6187050359712, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144271, Length=300, Percent_Identity=29.3333333333333, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=333, Percent_Identity=27.9279279279279, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25144274, Length=160, Percent_Identity=32.5, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=332, Percent_Identity=27.4096385542169, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=352, Percent_Identity=24.7159090909091, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21357415, Length=308, Percent_Identity=28.2467532467532, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI161085790, Length=344, Percent_Identity=27.906976744186, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574759, Length=344, Percent_Identity=27.906976744186, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356575, Length=326, Percent_Identity=28.8343558282209, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 43979; Mature: 43848

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGGVSSPVRAFKSVGGQPIVFDRVKGPFAWDVDGNRYIDYIGSWGPAICGHAHPEVITA
CCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCEEHHHCCCCCHHCCCCCHHHHHH
LQEAIEKGTSFGAPCVQENKLAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
VIKFDGCYHGHADMFLVKAGSGVATLGLPDSPGVPRTTTANTLTAPYNDLEAVKKLFSEN
EEEECCEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCC
PDAISGVILEPIVGNAGFITPEPGFLEGLRELTTENGSLLVFDEVMTGFRISYGGAQEKF
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHCCEEEECCCCCCCC
GVTPDLTTLGKVIGGGLPVGAYGGRKEIMSMVAPSGPVYQAGTLSGNPLAMTAGIKTLEL
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCEEEECCHHHHHH
LKQEGTYEKMGLATSRLIEGIMQSAENNGIAINGGSVSAMFGFFLCEGPVRNFEEAKTNN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHCCCC
SELFGKLHREMIKRGVYLAPSPFEAGFTSLAHSEEEIDRTIEAFDQSFSVIKN
HHHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGGVSSPVRAFKSVGGQPIVFDRVKGPFAWDVDGNRYIDYIGSWGPAICGHAHPEVITA
CCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCEEHHHCCCCCHHCCCCCHHHHHH
LQEAIEKGTSFGAPCVQENKLAEMVIDAVPSVEMVRFVNSGTEACMAVLRLMRAFTGRDK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
VIKFDGCYHGHADMFLVKAGSGVATLGLPDSPGVPRTTTANTLTAPYNDLEAVKKLFSEN
EEEECCEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCC
PDAISGVILEPIVGNAGFITPEPGFLEGLRELTTENGSLLVFDEVMTGFRISYGGAQEKF
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHCCEEEECCCCCCCC
GVTPDLTTLGKVIGGGLPVGAYGGRKEIMSMVAPSGPVYQAGTLSGNPLAMTAGIKTLEL
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCEEEECCHHHHHH
LKQEGTYEKMGLATSRLIEGIMQSAENNGIAINGGSVSAMFGFFLCEGPVRNFEEAKTNN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHCCCC
SELFGKLHREMIKRGVYLAPSPFEAGFTSLAHSEEEIDRTIEAFDQSFSVIKN
HHHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA