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The map label for this gene is amtB [C]

Identifier: 123965658

GI number: 123965658

Start: 378952

End: 380301

Strand: Reverse

Name: amtB [C]

Synonym: P9515_04231

Alternate gene names: 123965658

Gene position: 380301-378952 (Counterclockwise)

Preceding gene: 123965665

Following gene: 123965657

Centisome position: 22.32

GC content: 40.44

Gene sequence:

>1350_bases
TTGTTTAAAAAACTACCCGCCAAGATAGCAATAGCAACTCTTACTGTCCCAGCAATTCAGCTTATTTTTGCATCGCTCGC
GCCCATCCACGCTTCTGAATCAAATATTTCAGATGCTGACAATACATTAATTTTGACTTCATCTGCTCTGGTTTTACTAA
TGACTCCTGGTCTCGCTTTTTTCTATGGAGGTTTTACGCAGGCCAAGAATGTTCTAAATACGATGGGAATGAGTTTTGTG
ATGATGGGGATAGCTACCCTTGTTTGGTCAATAGTGGGGTTTAGTCTTGCCTTCTCTGATGGGGGAGCTTCAAATGCATT
TATAGGAAATCCATCAACCTATGCATTCTTAGAGAATCTTCCGCTTAATTGGGAGGGATTACAAATTCCAGGTCTTAGTT
TTGCTCTTTTTCAAGGCATGTTTGCAATAATCACTCCTGCTTTAATATCTGGGGCACTTGTAGAAAGAATTAGTTTCCGT
TTTTGGTGTTTCTTCACACCTATCTGGATTTTGTTTGTTTATGCACCACTTGCTCATATGGTTTGGGGAGGGGGACTATT
AGGAAAAGATCTTGATTTTGCAGGAGGTACAGTTGTTCATATAAGCTCTGGAGTCTCTGCTTTAGTTCTTGCTAGTCTGG
CTGGATCTCGCAGAAATTGGCCTAGAGGCATTAAGCCACCTCACGATATTACTCAAATACTATTAGGAACTGGTTTGCTA
TGGTTTGGTTGGTTTGGATTTAATGGAGGAAGTCAATTAAGCGTAGGAGGAGCAGAGCTCCCTTTCACAACCACTCATGT
TTCGGCCGCAGCTGGACTAGTAACTTGGGCCTTAATTGAGTCAATAAAAGATGGAAAAGCTTCTGCGATTGGAATGGCTA
CTGGTGCTGTAGCTGGATTAGTAGGAATTACCCCTGCAGCTGGTTTCGTAAATCCATCAAGTGGAATGTTAATTGGAGCA
ATTACCTCAGGTATTTGTTTTTTATCACTTAAATTAAAAATTAAATTATCTTTTGATGATTCTTTAGATACTTTTGCGGT
GCACGGTGTTGGAGGAACAGTTGGAGCTCTTCTAACTGGACTTTTTGCTAAGTCAGAGTTGATAGCTACTCATCCCGCAG
GATTAGTTCTTCAAGAACAGGGAAGGATTGCTTTGATTCTTGGTCAACTTCAGGCTGTTCTCATTGCATATGCAATTGCT
GCAATCGGAACTTTAATTATTGCTTTGGTTTTAAAATCTCTTGGTTGCAGTTTCAGAGTAAATGAAACAGATGAAGAGGA
TGGCTTAGATATCTCTCAACATGGTGAAGAGGCTTATGGAGAGAAGACAGGCTCGCCAAAAACTATTTAA

Upstream 100 bases:

>100_bases
TAAAGAATATGAGTTAATACTTAATTAATAAAAAATGTTCATTGTGAACATTTTTTATTGCGCCTCAATCTAATAATGTA
GCAACAGTTACTTTCTTATA

Downstream 100 bases:

>100_bases
ACAATCAAATTCTTGTTTTATAGCAATTATTTAATAATTGAAATTAATTGAATGGCAACTAAAAGATAAAATGTTTAGGG
ATTTTTACTCTCACGACTAA

Product: hypothetical protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MFKKLPAKIAIATLTVPAIQLIFASLAPIHASESNISDADNTLILTSSALVLLMTPGLAFFYGGFTQAKNVLNTMGMSFV
MMGIATLVWSIVGFSLAFSDGGASNAFIGNPSTYAFLENLPLNWEGLQIPGLSFALFQGMFAIITPALISGALVERISFR
FWCFFTPIWILFVYAPLAHMVWGGGLLGKDLDFAGGTVVHISSGVSALVLASLAGSRRNWPRGIKPPHDITQILLGTGLL
WFGWFGFNGGSQLSVGGAELPFTTTHVSAAAGLVTWALIESIKDGKASAIGMATGAVAGLVGITPAAGFVNPSSGMLIGA
ITSGICFLSLKLKIKLSFDDSLDTFAVHGVGGTVGALLTGLFAKSELIATHPAGLVLQEQGRIALILGQLQAVLIAYAIA
AIGTLIIALVLKSLGCSFRVNETDEEDGLDISQHGEEAYGEKTGSPKTI

Sequences:

>Translated_449_residues
MFKKLPAKIAIATLTVPAIQLIFASLAPIHASESNISDADNTLILTSSALVLLMTPGLAFFYGGFTQAKNVLNTMGMSFV
MMGIATLVWSIVGFSLAFSDGGASNAFIGNPSTYAFLENLPLNWEGLQIPGLSFALFQGMFAIITPALISGALVERISFR
FWCFFTPIWILFVYAPLAHMVWGGGLLGKDLDFAGGTVVHISSGVSALVLASLAGSRRNWPRGIKPPHDITQILLGTGLL
WFGWFGFNGGSQLSVGGAELPFTTTHVSAAAGLVTWALIESIKDGKASAIGMATGAVAGLVGITPAAGFVNPSSGMLIGA
ITSGICFLSLKLKIKLSFDDSLDTFAVHGVGGTVGALLTGLFAKSELIATHPAGLVLQEQGRIALILGQLQAVLIAYAIA
AIGTLIIALVLKSLGCSFRVNETDEEDGLDISQHGEEAYGEKTGSPKTI
>Mature_449_residues
MFKKLPAKIAIATLTVPAIQLIFASLAPIHASESNISDADNTLILTSSALVLLMTPGLAFFYGGFTQAKNVLNTMGMSFV
MMGIATLVWSIVGFSLAFSDGGASNAFIGNPSTYAFLENLPLNWEGLQIPGLSFALFQGMFAIITPALISGALVERISFR
FWCFFTPIWILFVYAPLAHMVWGGGLLGKDLDFAGGTVVHISSGVSALVLASLAGSRRNWPRGIKPPHDITQILLGTGLL
WFGWFGFNGGSQLSVGGAELPFTTTHVSAAAGLVTWALIESIKDGKASAIGMATGAVAGLVGITPAAGFVNPSSGMLIGA
ITSGICFLSLKLKIKLSFDDSLDTFAVHGVGGTVGALLTGLFAKSELIATHPAGLVLQEQGRIALILGQLQAVLIAYAIA
AIGTLIIALVLKSLGCSFRVNETDEEDGLDISQHGEEAYGEKTGSPKTI

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=437, Percent_Identity=39.1304347826087, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI133931100, Length=435, Percent_Identity=25.9770114942529, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=418, Percent_Identity=27.0334928229665, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17550182, Length=447, Percent_Identity=27.9642058165548, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17550180, Length=452, Percent_Identity=26.7699115044248, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324187, Length=412, Percent_Identity=37.1359223300971, Blast_Score=223, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=407, Percent_Identity=32.1867321867322, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321559, Length=407, Percent_Identity=32.6781326781327, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=28.6057692307692, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047
- InterPro:   IPR002229 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46854; Mature: 46854

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKLPAKIAIATLTVPAIQLIFASLAPIHASESNISDADNTLILTSSALVLLMTPGLAF
CCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEECCCHHH
FYGGFTQAKNVLNTMGMSFVMMGIATLVWSIVGFSLAFSDGGASNAFIGNPSTYAFLENL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHCC
PLNWEGLQIPGLSFALFQGMFAIITPALISGALVERISFRFWCFFTPIWILFVYAPLAHM
CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWGGGLLGKDLDFAGGTVVHISSGVSALVLASLAGSRRNWPRGIKPPHDITQILLGTGLL
HHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHH
WFGWFGFNGGSQLSVGGAELPFTTTHVSAAAGLVTWALIESIKDGKASAIGMATGAVAGL
HHHEECCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VGITPAAGFVNPSSGMLIGAITSGICFLSLKLKIKLSFDDSLDTFAVHGVGGTVGALLTG
HHCCCCCCCCCCCCCEEEEHHHCCEEEEEEEEEEEEEECCCCCCEEEECCCHHHHHHHHH
LFAKSELIATHPAGLVLQEQGRIALILGQLQAVLIAYAIAAIGTLIIALVLKSLGCSFRV
HHHHCCEEEECCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
NETDEEDGLDISQHGEEAYGEKTGSPKTI
CCCCCCCCCCHHHCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MFKKLPAKIAIATLTVPAIQLIFASLAPIHASESNISDADNTLILTSSALVLLMTPGLAF
CCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEECCCHHH
FYGGFTQAKNVLNTMGMSFVMMGIATLVWSIVGFSLAFSDGGASNAFIGNPSTYAFLENL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHCC
PLNWEGLQIPGLSFALFQGMFAIITPALISGALVERISFRFWCFFTPIWILFVYAPLAHM
CCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWGGGLLGKDLDFAGGTVVHISSGVSALVLASLAGSRRNWPRGIKPPHDITQILLGTGLL
HHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHH
WFGWFGFNGGSQLSVGGAELPFTTTHVSAAAGLVTWALIESIKDGKASAIGMATGAVAGL
HHHEECCCCCCEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VGITPAAGFVNPSSGMLIGAITSGICFLSLKLKIKLSFDDSLDTFAVHGVGGTVGALLTG
HHCCCCCCCCCCCCCEEEEHHHCCEEEEEEEEEEEEEECCCCCCEEEECCCHHHHHHHHH
LFAKSELIATHPAGLVLQEQGRIALILGQLQAVLIAYAIAAIGTLIIALVLKSLGCSFRV
HHHHCCEEEECCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
NETDEEDGLDISQHGEEAYGEKTGSPKTI
CCCCCCCCCCHHHCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]