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The map label for this gene is hemK [C]

Identifier: 123965585

GI number: 123965585

Start: 322806

End: 323675

Strand: Direct

Name: hemK [C]

Synonym: P9515_03501

Alternate gene names: 123965585

Gene position: 322806-323675 (Clockwise)

Preceding gene: 123965584

Following gene: 123965586

Centisome position: 18.94

GC content: 30.57

Gene sequence:

>870_bases
ATGTTCGAAATTTCTGCTGAAAACTTTTCGGTATGGAAAAAGAAACAACTCTTAAAAGGTGGTGATAAAAAATCGTTTAG
TCTTTTGATAGATTTATTGGGTGGATTATCAAAAAAAGAATTAAATTCACTCACAATAAAAGCAGAAAAAAATTTAAAAT
TTAAATTAGATTTAGACTCCCTTGAATTTTTTTGGGGGAAGCATCTCAGTACTAATATTCCTATTCAATATTTAAGTGGT
ATTTGTTATTGGAGAAATCTCAAATTAGAGGTTTCAGATAAGGTTCTAATACCACGTCCGGAAACAGAACTTGTGGTTGA
GATAATATCGAGAAAATTTGATAATAATAAAGGACAAATTATTTTTGCAGATTTAGGAACTGGTTCAGGTGCAATTAGTA
TCTCGTTAGCTTTAGAAAATCCAAGCTGGAATGGAATTGCAACGGATATTAATAAAAATGCTGTAGAGATAGCCTCGAGA
AACTTTGCTAATAACTCTAATCAATCTAATTTGAAATTTTATAGTGGGAATTGGTGGGAGCCTCTAACATATCTCAAAGG
GGAGATAGATTTTGCGGTAGCTAATCCTCCATATATTCCAAAAAATACTTATGAAGAATTACCAATAGAGGTAAAGAATT
TTGAACCTAAGAATGCTCTTTTGGGAGGAGAAGATGGTTTGGATCACGTAAGGGAAATAGTTAAATATGCCCCTTTATAC
TTAAAAGAAAAAGGTTGGTTATTAATTGAGAATCATTTTGATCAAGGTGCCAGAGTAAAACAATTATTTATTGAAAATCA
ATTTACTGATGTAGAAGTATTAAAAGATTTGTCTGGAATTGGAAGATTTACAATTGGAAGATATAAATAA

Upstream 100 bases:

>100_bases
GATTGGTTTATTTATTTCCACGCAAGATGGAGAAAAATCAAGCTTTTACTCAGATTCCGGTAAAGGTAAACTTGGCCCAA
AGCGCTAATTAAGTTATTTA

Downstream 100 bases:

>100_bases
TTTATTAGGAGAAAACGAATGAATGTTATTGCTAATAAATTAGCTGTAGAAAAACTAAATATTGGCTTACCTATAATTTT
TCATACTGATACTTTACCTG

Product: putative protein methyltransferase

Products: Protoporphyrin IX; H2O

Alternate protein names: M.Ssp6803HemKP [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MFEISAENFSVWKKKQLLKGGDKKSFSLLIDLLGGLSKKELNSLTIKAEKNLKFKLDLDSLEFFWGKHLSTNIPIQYLSG
ICYWRNLKLEVSDKVLIPRPETELVVEIISRKFDNNKGQIIFADLGTGSGAISISLALENPSWNGIATDINKNAVEIASR
NFANNSNQSNLKFYSGNWWEPLTYLKGEIDFAVANPPYIPKNTYEELPIEVKNFEPKNALLGGEDGLDHVREIVKYAPLY
LKEKGWLLIENHFDQGARVKQLFIENQFTDVEVLKDLSGIGRFTIGRYK

Sequences:

>Translated_289_residues
MFEISAENFSVWKKKQLLKGGDKKSFSLLIDLLGGLSKKELNSLTIKAEKNLKFKLDLDSLEFFWGKHLSTNIPIQYLSG
ICYWRNLKLEVSDKVLIPRPETELVVEIISRKFDNNKGQIIFADLGTGSGAISISLALENPSWNGIATDINKNAVEIASR
NFANNSNQSNLKFYSGNWWEPLTYLKGEIDFAVANPPYIPKNTYEELPIEVKNFEPKNALLGGEDGLDHVREIVKYAPLY
LKEKGWLLIENHFDQGARVKQLFIENQFTDVEVLKDLSGIGRFTIGRYK
>Mature_289_residues
MFEISAENFSVWKKKQLLKGGDKKSFSLLIDLLGGLSKKELNSLTIKAEKNLKFKLDLDSLEFFWGKHLSTNIPIQYLSG
ICYWRNLKLEVSDKVLIPRPETELVVEIISRKFDNNKGQIIFADLGTGSGAISISLALENPSWNGIATDINKNAVEIASR
NFANNSNQSNLKFYSGNWWEPLTYLKGEIDFAVANPPYIPKNTYEELPIEVKNFEPKNALLGGEDGLDHVREIVKYAPLY
LKEKGWLLIENHFDQGARVKQLFIENQFTDVEVLKDLSGIGRFTIGRYK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=226, Percent_Identity=30.9734513274336, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787463, Length=215, Percent_Identity=35.3488372093023, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87082085, Length=187, Percent_Identity=30.4812834224599, Blast_Score=79, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582226, Length=198, Percent_Identity=28.2828282828283, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 1.3.3.4

Molecular weight: Translated: 32851; Mature: 32851

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEISAENFSVWKKKQLLKGGDKKSFSLLIDLLGGLSKKELNSLTIKAEKNLKFKLDLDS
CEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCH
LEFFWGKHLSTNIPIQYLSGICYWRNLKLEVSDKVLIPRPETELVVEIISRKFDNNKGQI
HHHHHCCCCCCCCCHHHHCCEEEEEEEEEEECCEEEECCCCHHHHHHHHHHHCCCCCCEE
IFADLGTGSGAISISLALENPSWNGIATDINKNAVEIASRNFANNSNQSNLKFYSGNWWE
EEEECCCCCCEEEEEEEEECCCCCCEEECCCCCHHHHHHCCCCCCCCCCCEEEECCCCCC
PLTYLKGEIDFAVANPPYIPKNTYEELPIEVKNFEPKNALLGGEDGLDHVREIVKYAPLY
CHHHCCCEEEEEECCCCCCCCCCHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEE
LKEKGWLLIENHFDQGARVKQLFIENQFTDVEVLKDLSGIGRFTIGRYK
EECCCCEEEECCCCCCCCEEHHHHCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFEISAENFSVWKKKQLLKGGDKKSFSLLIDLLGGLSKKELNSLTIKAEKNLKFKLDLDS
CEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEECCH
LEFFWGKHLSTNIPIQYLSGICYWRNLKLEVSDKVLIPRPETELVVEIISRKFDNNKGQI
HHHHHCCCCCCCCCHHHHCCEEEEEEEEEEECCEEEECCCCHHHHHHHHHHHCCCCCCEE
IFADLGTGSGAISISLALENPSWNGIATDINKNAVEIASRNFANNSNQSNLKFYSGNWWE
EEEECCCCCCEEEEEEEEECCCCCCEEECCCCCHHHHHHCCCCCCCCCCCEEEECCCCCC
PLTYLKGEIDFAVANPPYIPKNTYEELPIEVKNFEPKNALLGGEDGLDHVREIVKYAPLY
CHHHCCCEEEEEECCCCCCCCCCHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEE
LKEKGWLLIENHFDQGARVKQLFIENQFTDVEVLKDLSGIGRFTIGRYK
EECCCCEEEECCCCCCCCEEHHHHCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protoporphyrinogen IX; O2

Specific reaction: protoporphyrinogen-IX + 1.5 O2 = protoporphyrin-IX + 3 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]