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The map label for this gene is pyrB

Identifier: 123965500

GI number: 123965500

Start: 245404

End: 246420

Strand: Reverse

Name: pyrB

Synonym: P9515_02651

Alternate gene names: 123965500

Gene position: 246420-245404 (Counterclockwise)

Preceding gene: 123965501

Following gene: 123965497

Centisome position: 14.46

GC content: 33.92

Gene sequence:

>1017_bases
ATGGGAAATTGGCCTCACAAACATATCCTCACACTCTCAAATTTCTCAATCTATGATTATAAATCAGTTTTTGAGTTAAC
TGAAAGATTCAAATCTCTAATTAATGCTGGTACAAAAAAAATACCTGCTTTACAAGGAAATTTAATAACCTCAATATTTT
TTGAGGCCAGCACTAGAACGCGTAACAGCTTTGAGCTCGCGGCCAAAAGGCTTTCTGCTGACGTTCAAAGCTTTTCTCCT
TCTTCAAGTTCTTTGAGTAAAGGAGAAACTCTTATAGATACTGCATTAACATACGCCGCAATGGGATCAGATATACTAAT
CATTAGACATTCATCTAGTCATGTACCTCTAGAAATTTCAAAAAAACTAGATGCAACTAATACAAAAACTTCGGTGCTCA
ATGCTGGTGATGGTTTACATAGCCATCCTAGTCAAGGATTACTAGATCTTTATACATTAATAAAGTTCTTTTCTCCTGAA
TTGATGAAACCAGAGATCTTATATTCTAAAAAAATTTTAATAGTGGGGGATGTATTGCATTCAAGAGTTGCCCGATCAAA
TCTTTGGGCATTGACTGCATTTGGTGCGAACGTAATCTTGTGTGGCCCTCCAACACTTATTCCTGAAGAATTTACTAGTT
TTGTAAGTAGTTCTCCTCCCAATCAATTAAGAGACCCAATTTCTTCTAGGGGTTCCATTACAATTTCGAGGTCATTAGAA
GACTCCATTAAAGATGCAGATGCGGTGATTGTTTTAAGATTACAAAAAGAAAGAATGATGGAGAATTTATTAAGCAGTAT
CAAATCTTATAGTGAAAATTATTGTTTAACTCCAGAAAAGCTATCTATGAATGATAAAAATATTCCTATCCTGCATCCTG
GGCCTATTAATCGTGGTATTGAAATTAGCAGCAGAATAGTTGATGAATATCCAAATTGCTTAATAAATGATCAAGTCTCT
AATGGAATTCCTACAAGAATGGCTTTACTTTATTTATTAAGCAAATTCAATAAATAA

Upstream 100 bases:

>100_bases
GTTGGTATCTTAAAAGGAGTAGAAGTATTAGTAAAAGAGAAAAAGGAGATAGAAATCCTGATTTAAAAAAATCAACTAAT
AATTTGCTTGGGATAACATA

Downstream 100 bases:

>100_bases
ATTCAAAGTAATTTAAGGCTTTCGGTAAAAAAACCATAAAACAATCCCAATCTTAATGAATCGATTAACAATACTAATAA
TTTTTTTCTCCTATCAAACC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGNWPHKHILTLSNFSIYDYKSVFELTERFKSLINAGTKKIPALQGNLITSIFFEASTRTRNSFELAAKRLSADVQSFSP
SSSSLSKGETLIDTALTYAAMGSDILIIRHSSSHVPLEISKKLDATNTKTSVLNAGDGLHSHPSQGLLDLYTLIKFFSPE
LMKPEILYSKKILIVGDVLHSRVARSNLWALTAFGANVILCGPPTLIPEEFTSFVSSSPPNQLRDPISSRGSITISRSLE
DSIKDADAVIVLRLQKERMMENLLSSIKSYSENYCLTPEKLSMNDKNIPILHPGPINRGIEISSRIVDEYPNCLINDQVS
NGIPTRMALLYLLSKFNK

Sequences:

>Translated_338_residues
MGNWPHKHILTLSNFSIYDYKSVFELTERFKSLINAGTKKIPALQGNLITSIFFEASTRTRNSFELAAKRLSADVQSFSP
SSSSLSKGETLIDTALTYAAMGSDILIIRHSSSHVPLEISKKLDATNTKTSVLNAGDGLHSHPSQGLLDLYTLIKFFSPE
LMKPEILYSKKILIVGDVLHSRVARSNLWALTAFGANVILCGPPTLIPEEFTSFVSSSPPNQLRDPISSRGSITISRSLE
DSIKDADAVIVLRLQKERMMENLLSSIKSYSENYCLTPEKLSMNDKNIPILHPGPINRGIEISSRIVDEYPNCLINDQVS
NGIPTRMALLYLLSKFNK
>Mature_337_residues
GNWPHKHILTLSNFSIYDYKSVFELTERFKSLINAGTKKIPALQGNLITSIFFEASTRTRNSFELAAKRLSADVQSFSPS
SSSLSKGETLIDTALTYAAMGSDILIIRHSSSHVPLEISKKLDATNTKTSVLNAGDGLHSHPSQGLLDLYTLIKFFSPEL
MKPEILYSKKILIVGDVLHSRVARSNLWALTAFGANVILCGPPTLIPEEFTSFVSSSPPNQLRDPISSRGSITISRSLED
SIKDADAVIVLRLQKERMMENLLSSIKSYSENYCLTPEKLSMNDKNIPILHPGPINRGIEISSRIVDEYPNCLINDQVSN
GIPTRMALLYLLSKFNK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=328, Percent_Identity=30.7926829268293, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI2367364, Length=332, Percent_Identity=31.3253012048193, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786469, Length=350, Percent_Identity=25.1428571428571, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790703, Length=256, Percent_Identity=25.78125, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=343, Percent_Identity=29.4460641399417, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322331, Length=332, Percent_Identity=29.5180722891566, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24642586, Length=335, Percent_Identity=31.6417910447761, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PROM5 (A2BUL3)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010581.1
- ProteinModelPortal:   A2BUL3
- SMR:   A2BUL3
- STRING:   A2BUL3
- GeneID:   4720401
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_02651
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   LYFAGPE
- ProtClustDB:   PRK00856
- BioCyc:   PMAR167542:P9515ORF_0274-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37447; Mature: 37315

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNWPHKHILTLSNFSIYDYKSVFELTERFKSLINAGTKKIPALQGNLITSIFFEASTRT
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCC
RNSFELAAKRLSADVQSFSPSSSSLSKGETLIDTALTYAAMGSDILIIRHSSSHVPLEIS
CHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEC
KKLDATNTKTSVLNAGDGLHSHPSQGLLDLYTLIKFFSPELMKPEILYSKKILIVGDVLH
CCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEHHHHH
SRVARSNLWALTAFGANVILCGPPTLIPEEFTSFVSSSPPNQLRDPISSRGSITISRSLE
HHHHHCCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCHH
DSIKDADAVIVLRLQKERMMENLLSSIKSYSENYCLTPEKLSMNDKNIPILHPGPINRGI
HHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCEECCCCCCCCC
EISSRIVDEYPNCLINDQVSNGIPTRMALLYLLSKFNK
CHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNWPHKHILTLSNFSIYDYKSVFELTERFKSLINAGTKKIPALQGNLITSIFFEASTRT
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCC
RNSFELAAKRLSADVQSFSPSSSSLSKGETLIDTALTYAAMGSDILIIRHSSSHVPLEIS
CHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEC
KKLDATNTKTSVLNAGDGLHSHPSQGLLDLYTLIKFFSPELMKPEILYSKKILIVGDVLH
CCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEHHHHH
SRVARSNLWALTAFGANVILCGPPTLIPEEFTSFVSSSPPNQLRDPISSRGSITISRSLE
HHHHHCCCEEEEECCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCHH
DSIKDADAVIVLRLQKERMMENLLSSIKSYSENYCLTPEKLSMNDKNIPILHPGPINRGI
HHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCEECCCCCCCCC
EISSRIVDEYPNCLINDQVSNGIPTRMALLYLLSKFNK
CHHHHHHHHCCCCEECCHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA