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The map label for this gene is ndhI [H]

Identifier: 123965423

GI number: 123965423

Start: 171812

End: 172438

Strand: Reverse

Name: ndhI [H]

Synonym: P9515_01881

Alternate gene names: 123965423

Gene position: 172438-171812 (Counterclockwise)

Preceding gene: 123965424

Following gene: 123965422

Centisome position: 10.12

GC content: 33.81

Gene sequence:

>627_bases
ATGAAAGACTTTCTTCAAAAAGTAAATAGCTATATTAAAGAAGCCTTTGGAGCTGGTAAATATTTATATGATGGACTTTC
TGTAACTTTTGATCATCTCCGAAGAAGGCCAGTTACTGTTCAATACCCTTACGAGAAGTTAATACCATCTGAAAGATATA
GAGGAAGAATCCATTATGAATTTGATAAATGTATAGCCTGTGAAGTATGCGTACGTGTTTGTCCCATAAATCTGCCAGTA
GTAGATTGGGTAATGAATAAAGAAACTAAAAAAAAGGAACTTAGAAATTACTCTATAGACTTTGGAGTATGTATTTTTTG
TGGGAATTGTGTTGAATATTGTCCAACAAATTGCTTATCCATGACTGAAGAATATGAATTAGCGACATTCGATAGACATA
ATTTAAATTTTGATAATGTGGCTTTAGGCAGACTTCCTACAAACGTTACGACTGATCCTTCTGTAAAACCTCTTAGAGAA
CTTGCATATTTACCTAAAGGAGTTATGGATCCTCATGAAGTCTCTTCTTCCGATGCTCGTGTTGGTAAATTACCTGAAGA
AGTTTATGATTGGATGAAACCAAAAACGAATGAAATGAGTGATCCCGCCGTCAAACTAAATAAATAA

Upstream 100 bases:

>100_bases
ACTAGCTCTCCCTCAATTTTTTGGTGGATAATATTTAAGTAAATGTACTTAAATACTAAATTAATCCACTACACTAAAGA
CACTAAGTTATCACTTTAAA

Downstream 100 bases:

>100_bases
TTAACTACATAAATGTCTATAGCAGTCACAACACAAATTATTTGTTTTTCAGTATTGTCTTTAGTTGTAATAGTTGGAGC
ACTAGGGGTAATTCTTCTTG

Product: NADH dehydrogenase subunit I

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKDFLQKVNSYIKEAFGAGKYLYDGLSVTFDHLRRRPVTVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELATFDRHNLNFDNVALGRLPTNVTTDPSVKPLRE
LAYLPKGVMDPHEVSSSDARVGKLPEEVYDWMKPKTNEMSDPAVKLNK

Sequences:

>Translated_208_residues
MKDFLQKVNSYIKEAFGAGKYLYDGLSVTFDHLRRRPVTVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELATFDRHNLNFDNVALGRLPTNVTTDPSVKPLRE
LAYLPKGVMDPHEVSSSDARVGKLPEEVYDWMKPKTNEMSDPAVKLNK
>Mature_208_residues
MKDFLQKVNSYIKEAFGAGKYLYDGLSVTFDHLRRRPVTVQYPYEKLIPSERYRGRIHYEFDKCIACEVCVRVCPINLPV
VDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELATFDRHNLNFDNVALGRLPTNVTTDPSVKPLRE
LAYLPKGVMDPHEVSSSDARVGKLPEEVYDWMKPKTNEMSDPAVKLNK

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=126, Percent_Identity=31.7460317460317, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789075, Length=98, Percent_Identity=33.6734693877551, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788617, Length=117, Percent_Identity=31.6239316239316, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=126, Percent_Identity=30.1587301587302, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17864306, Length=126, Percent_Identity=30.952380952381, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 24053; Mature: 24053

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFLQKVNSYIKEAFGAGKYLYDGLSVTFDHLRRRPVTVQYPYEKLIPSERYRGRIHYE
CHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCEEECCHHHCCCCHHHCCCEEEC
FDKCIACEVCVRVCPINLPVVDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCHHHEEHCCCHHHHCCCHHHH
MTEEYELATFDRHNLNFDNVALGRLPTNVTTDPSVKPLRELAYLPKGVMDPHEVSSSDAR
HHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCCH
VGKLPEEVYDWMKPKTNEMSDPAVKLNK
HCCCHHHHHHHHCCCCCCCCCCCEECCC
>Mature Secondary Structure
MKDFLQKVNSYIKEAFGAGKYLYDGLSVTFDHLRRRPVTVQYPYEKLIPSERYRGRIHYE
CHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCEEECCHHHCCCCHHHCCCEEEC
FDKCIACEVCVRVCPINLPVVDWVMNKETKKKELRNYSIDFGVCIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCHHHEEHCCCHHHHCCCHHHH
MTEEYELATFDRHNLNFDNVALGRLPTNVTTDPSVKPLRELAYLPKGVMDPHEVSSSDAR
HHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCCH
VGKLPEEVYDWMKPKTNEMSDPAVKLNK
HCCCHHHHHHHHCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]