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The map label for this gene is metF [H]

Identifier: 123965416

GI number: 123965416

Start: 168008

End: 168898

Strand: Reverse

Name: metF [H]

Synonym: P9515_01811

Alternate gene names: 123965416

Gene position: 168898-168008 (Counterclockwise)

Preceding gene: 123965418

Following gene: 123965410

Centisome position: 9.91

GC content: 32.88

Gene sequence:

>891_bases
TTGAAATCAAAACTTCAGCAAACTTTAGAAAAAAATTCAAAAGTAATTACAGCAGAATTAATGCCGCCAAGAGGAGGGGA
CCCCATAAGATCTCTTAAAATAGCACAACTTCTGAAAAATAAGGTACATGCTGTTAACATAACAGACGGAAGTAGAGCAA
TAATGAGGATGTGTAGTTTAGCAATGTCTAAACTATTACTGGAAAATGGGATAGAACCGATAATGCAAATTTCTTGCAGA
GATCGTAATAAAATTGCTTTACAATCAGATATTCTTGGAGCAAATGCATTAGGTATTAAAAATATTTTATGCATTACAGG
AGATTCAGTAAAAGCTGGTGATCAGCAAGAAACAAAAGCAGTTCATGAATTTGAAGCAGTTCGACTATTAAAACAAATTC
AATATTTCAATCAAGGTATTGATCCTACTTTTGAAAAACTTGCTGACAATAGAACTAACATATTTGCTGGTGCTGCAGTA
GATCCCAGTTGTCGGAATCAAAGAAGTTTAAAAAATCGAACAAGAAAGAAGAAGGAGGCAGGAGCAAATTTTTTACAAAC
TCAAATAGTTATGGATAGAAATTATTTGATAGATTTTTGCCAAGAAATCAGTAATCCACTTGAGATACCAGTTATAGCAG
GAGTATTTCTTTTGAAATCATATAAAAATGCCCTCTTCATAAATAAATTTGTACCTGGCGCGAATATTCCTGAAAATATT
TTAAATCGTCTCAAAGAAGCAAAAGACCCCCTGAAAGAAGGAATATTAATTGCTGCTGAACAAGCTAAAGATTTTATTAA
CATCGCTGAAGGTATTCATATAATGGCAGTCAAGGCAGAAAATCTTATCCCAGAAATACTTGAAAAAGCTGGTCTTAATC
TGGAATGTTAA

Upstream 100 bases:

>100_bases
AATACCTTCTTCAACCATTTGCATTTAAACAGTTCCTTTTATATATTAATTGAGTTTAGCAGTATCAGTAAACTTCTTAA
CCAAGTAGGGAAAAAAGCAA

Downstream 100 bases:

>100_bases
TTAAATCAGATCCTAATAATTCTGCCATGGCTTTTTGCTGAGGTGATGGCTCAGTTAAATCTAATCTTCTTGAATCTGTT
ATTAACCAGTCCAAAGAAGC

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKSKLQQTLEKNSKVITAELMPPRGGDPIRSLKIAQLLKNKVHAVNITDGSRAIMRMCSLAMSKLLLENGIEPIMQISCR
DRNKIALQSDILGANALGIKNILCITGDSVKAGDQQETKAVHEFEAVRLLKQIQYFNQGIDPTFEKLADNRTNIFAGAAV
DPSCRNQRSLKNRTRKKKEAGANFLQTQIVMDRNYLIDFCQEISNPLEIPVIAGVFLLKSYKNALFINKFVPGANIPENI
LNRLKEAKDPLKEGILIAAEQAKDFINIAEGIHIMAVKAENLIPEILEKAGLNLEC

Sequences:

>Translated_296_residues
MKSKLQQTLEKNSKVITAELMPPRGGDPIRSLKIAQLLKNKVHAVNITDGSRAIMRMCSLAMSKLLLENGIEPIMQISCR
DRNKIALQSDILGANALGIKNILCITGDSVKAGDQQETKAVHEFEAVRLLKQIQYFNQGIDPTFEKLADNRTNIFAGAAV
DPSCRNQRSLKNRTRKKKEAGANFLQTQIVMDRNYLIDFCQEISNPLEIPVIAGVFLLKSYKNALFINKFVPGANIPENI
LNRLKEAKDPLKEGILIAAEQAKDFINIAEGIHIMAVKAENLIPEILEKAGLNLEC
>Mature_296_residues
MKSKLQQTLEKNSKVITAELMPPRGGDPIRSLKIAQLLKNKVHAVNITDGSRAIMRMCSLAMSKLLLENGIEPIMQISCR
DRNKIALQSDILGANALGIKNILCITGDSVKAGDQQETKAVHEFEAVRLLKQIQYFNQGIDPTFEKLADNRTNIFAGAAV
DPSCRNQRSLKNRTRKKKEAGANFLQTQIVMDRNYLIDFCQEISNPLEIPVIAGVFLLKSYKNALFINKFVPGANIPENI
LNRLKEAKDPLKEGILIAAEQAKDFINIAEGIHIMAVKAENLIPEILEKAGLNLEC

Specific function: Unknown

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 32874; Mature: 32874

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKLQQTLEKNSKVITAELMPPRGGDPIRSLKIAQLLKNKVHAVNITDGSRAIMRMCSL
CCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
AMSKLLLENGIEPIMQISCRDRNKIALQSDILGANALGIKNILCITGDSVKAGDQQETKA
HHHHHHHHHCCCHHHHHHCCCCCCEEEHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHH
VHEFEAVRLLKQIQYFNQGIDPTFEKLADNRTNIFAGAAVDPSCRNQRSLKNRTRKKKEA
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
GANFLQTQIVMDRNYLIDFCQEISNPLEIPVIAGVFLLKSYKNALFINKFVPGANIPENI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHH
LNRLKEAKDPLKEGILIAAEQAKDFINIAEGIHIMAVKAENLIPEILEKAGLNLEC
HHHHHHHHHHHHHCEEEEHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSKLQQTLEKNSKVITAELMPPRGGDPIRSLKIAQLLKNKVHAVNITDGSRAIMRMCSL
CCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
AMSKLLLENGIEPIMQISCRDRNKIALQSDILGANALGIKNILCITGDSVKAGDQQETKA
HHHHHHHHHCCCHHHHHHCCCCCCEEEHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHH
VHEFEAVRLLKQIQYFNQGIDPTFEKLADNRTNIFAGAAVDPSCRNQRSLKNRTRKKKEA
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
GANFLQTQIVMDRNYLIDFCQEISNPLEIPVIAGVFLLKSYKNALFINKFVPGANIPENI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHH
LNRLKEAKDPLKEGILIAAEQAKDFINIAEGIHIMAVKAENLIPEILEKAGLNLEC
HHHHHHHHHHHHHCEEEEHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]