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The map label for this gene is cysH [H]

Identifier: 123965326

GI number: 123965326

Start: 95571

End: 96296

Strand: Reverse

Name: cysH [H]

Synonym: P9515_00911

Alternate gene names: 123965326

Gene position: 96296-95571 (Counterclockwise)

Preceding gene: 123965331

Following gene: 123965325

Centisome position: 5.65

GC content: 31.4

Gene sequence:

>726_bases
ATGATGATTGAGAAAACTCTAGAAAGTATAAAACTTAATTTAGAAGAGCAAAATAAAAAATTAAAAGATATGACCTCAAA
AGAAATAATGTTATGGGGTTATAGAAACTTTGATAATCACTTTGCTATTACTACAAGCTTTGGGATACAGTCATCAGTGA
TATTGCATATGGTGAATGAATGCTCATTACAAAAGAAAATTAAAATCTTTTGGATAGATACAGGTTACTTACCCTCAGAG
ACATATAAATATGCAGAGAGACTTATTAATGATTTATCTTTAGAGATTGAGGTTTTACAAAGTGAAATATCGCCGGCAAG
AATGGAATCGCTATATGGCAAACTTTGGGAAACTAATAAATCTAGTGACTTAGATAAATACCATGACATAAGAAAAGTCA
AACCTTTAGAAAAGGCTTTAGATAGTCATAACATTAATTGCTGGGCTAGTGGTGTGAGATCAGAACAAACGAATAATAGA
AATAAAATGAAATTTGTTGATGTAATACGAAACCGACTTTCTTTAAGGCCATTACTGAACTGGAAAAACAAAGATATTTA
TTATTACATGAAAGAAAATCAATTACCTTCTCACCCACTTTTTTCAAAAGGTTATTCAACTGTAGGTGATTGGCATTCTA
GCTCTCCTGATAGTATAGAGAATGAAGGCAGGTCAACAAGATTTAGGGGGATGAAGCAAGAGTGTGGAATTCATACTGAA
AATTAA

Upstream 100 bases:

>100_bases
TGATTTCATGAAGGCTATATTGTTAATACTAAATGTATTATTAAATTAAACGAATAATCTTCAAACTGAGCGTATAACCT
CAAAATCCTAACTACCTTTG

Downstream 100 bases:

>100_bases
AATTTTCTTTCAGGATCATGTTCGAAAGAAATCATTTTTTATTAGTTGGCAATAGCAGACTTCACTGGGCAGAAAATTTA
AAAAATCAGTATAGGTTCTT

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MMIEKTLESIKLNLEEQNKKLKDMTSKEIMLWGYRNFDNHFAITTSFGIQSSVILHMVNECSLQKKIKIFWIDTGYLPSE
TYKYAERLINDLSLEIEVLQSEISPARMESLYGKLWETNKSSDLDKYHDIRKVKPLEKALDSHNINCWASGVRSEQTNNR
NKMKFVDVIRNRLSLRPLLNWKNKDIYYYMKENQLPSHPLFSKGYSTVGDWHSSSPDSIENEGRSTRFRGMKQECGIHTE
N

Sequences:

>Translated_241_residues
MMIEKTLESIKLNLEEQNKKLKDMTSKEIMLWGYRNFDNHFAITTSFGIQSSVILHMVNECSLQKKIKIFWIDTGYLPSE
TYKYAERLINDLSLEIEVLQSEISPARMESLYGKLWETNKSSDLDKYHDIRKVKPLEKALDSHNINCWASGVRSEQTNNR
NKMKFVDVIRNRLSLRPLLNWKNKDIYYYMKENQLPSHPLFSKGYSTVGDWHSSSPDSIENEGRSTRFRGMKQECGIHTE
N
>Mature_241_residues
MMIEKTLESIKLNLEEQNKKLKDMTSKEIMLWGYRNFDNHFAITTSFGIQSSVILHMVNECSLQKKIKIFWIDTGYLPSE
TYKYAERLINDLSLEIEVLQSEISPARMESLYGKLWETNKSSDLDKYHDIRKVKPLEKALDSHNINCWASGVRSEQTNNR
NKMKFVDVIRNRLSLRPLLNWKNKDIYYYMKENQLPSHPLFSKGYSTVGDWHSSSPDSIENEGRSTRFRGMKQECGIHTE
N

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=230, Percent_Identity=36.5217391304348, Blast_Score=168, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6325425, Length=225, Percent_Identity=28.8888888888889, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 28312; Mature: 28312

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIEKTLESIKLNLEEQNKKLKDMTSKEIMLWGYRNFDNHFAITTSFGIQSSVILHMVNE
CCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHH
CSLQKKIKIFWIDTGYLPSETYKYAERLINDLSLEIEVLQSEISPARMESLYGKLWETNK
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SSDLDKYHDIRKVKPLEKALDSHNINCWASGVRSEQTNNRNKMKFVDVIRNRLSLRPLLN
CCCHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
WKNKDIYYYMKENQLPSHPLFSKGYSTVGDWHSSSPDSIENEGRSTRFRGMKQECGIHTE
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCHHHCCCCCC
N
C
>Mature Secondary Structure
MMIEKTLESIKLNLEEQNKKLKDMTSKEIMLWGYRNFDNHFAITTSFGIQSSVILHMVNE
CCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHH
CSLQKKIKIFWIDTGYLPSETYKYAERLINDLSLEIEVLQSEISPARMESLYGKLWETNK
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SSDLDKYHDIRKVKPLEKALDSHNINCWASGVRSEQTNNRNKMKFVDVIRNRLSLRPLLN
CCCHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
WKNKDIYYYMKENQLPSHPLFSKGYSTVGDWHSSSPDSIENEGRSTRFRGMKQECGIHTE
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCHHHCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1463852 [H]