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The map label for this gene is priA [H]

Identifier: 121999046

GI number: 121999046

Start: 2487434

End: 2489671

Strand: Reverse

Name: priA [H]

Synonym: Hhal_2267

Alternate gene names: 121999046

Gene position: 2489671-2487434 (Counterclockwise)

Preceding gene: 121999048

Following gene: 121999045

Centisome position: 92.95

GC content: 73.82

Gene sequence:

>2238_bases
GTGCCTCCCCAGGGCGGTCACCCCCAAGGGGCGGGCGCGCCGCCCATCCTTCAGGTGGCGGTGCCCCGTCCCCTGCAAGA
GGCGCTGGACTATCGACCACCCCCCGGCGCCTGGCCGGCTGAACCGGTGGGGTGCCGGGTCGAGGTCCCCCTGGGCCGCA
GCCGCGCGGTGGGTATTGTGGTGGCGGTTCGCCACAGCTCGGATCTACCGGCATCGCGCTTGCGCGCCGCCAGCGCCTGG
GTGGATGAGCAGCCGCTGCTCGATACGGAACTGCTGGAGACGCTCACCTGGGCGGCGCGCTACTACCGCTATCCGCTGGG
CACAGCCCTGGCTACCGCGCTGCCGGGCCCACTGCGTCAGGGCCGGCCGGCCCGAAGGGCCGTGGCCTGGTGGCGCCTGG
ACGCGCGGCTGCACGAGGACGATCGTGCGGTGCGCCGGGCGCCGCGGCAGGCAGAGCTGCGCCGCCGACTGCTGGCATCG
GGGGGCGCGGCGCCATACGCGTTGCTGGTGGCGGAGCAGCCGGCCTGGCAGCGGGCCGGCCGCCTCCTTGAGCAGGCCGG
TCTGCTCCACCGCGAGTATCACCCGGCCCAGGCCGAGGGCAGCGCGGACCGGACGCCGCGCCCCGGGCCGGAGCTCGACG
CCGACCAACAGCAGGCCCTGGAGTGCATGCTCGCCGCCGACGGCTACGCGGCCTTCCTGCTTGAGGGCGTGACCGGCAGC
GGCAAGACCGAGGTCTATCTGCAGACGGCCGCTCGGGCCCTGGCGGCGGGGCGGCAGGTGCTCATCCTGGTGCCCGAGAT
CGGCCTGGCCCCGCAGTTCCTGGAGCGTCTGCGCGAGCGCCTGAGCGGCTCGGTGGTTGTGCTCCATTCCGGGCTGTCCG
GCACCGACCGTGCCGAGGCCTGGCTGGCGGCCCGAGACGGCGACGCGGCGGTGATCCTGGGGACCCGCTCGGCGATCTTC
ACCCCGCTGCCCCGTCCGGGACTGATCGTGGTCGACGAGGAGCACGACCCTTCGCTGGCCCAGCAGGACGGCTTCCGCTA
TTCGGCGCGGGATCTGGCCGTGCTGCGGGCTCGCACCCTGGATGTGCCGGTGGTACTCGGCTCTGCGACCCCGTCGCTGG
AGTCGCTGCACAATGCCCGCGGCGCTCGGTATACCCATCTGCGCCTGCCCCATCGGGCGGGGCTGGCCCGCGCTCCGAGT
GTGCAGCTGGTCGACATCCGCAGCCGCCGCCTCGTCGGCGGCATTTCGGCACCGCTGCAGGAGGCGGTGCGCCGGCACCT
GGATGCCGGGTCGCAGGTCCTGTTGTTCCTCAACCGGCGCGGATACGCCCCGGTGCTGATCTGCCACGCCTGTGGCTGGG
TCGCCGGGTGCCACCGCTGCGATGCGCGGCTGACCTACCACCGCAGCCGCGCCCAGCTGCGCTGCCACCACTGCGGCCAT
ACCGCGCGCGTGCCCGCGGCCTGTCCGGAGTGCGGCGAGGCCGACCTGCGTCCCTTGGGGCCGGGGACGGAACGCCTTGA
GGAGGCGCTCGCCGAGCTCTTCCCCGGGGTGGGCCAGCTGCGGATTGATCGGGATACCACCCGCCGCCGCGGAGCGTTAG
AGGCCCAGCTGGCGGCGGCGCACCGCGGCGACGCGCAACTGCTCATCGGCACGCAGATGCTCGCCAAGGGGCACCACCTG
CCGGCGGTCACCCTGGTGGCGGTGATCGACGTCGATCAGGGGCTGTTCGGTGCGGACTTCCGGGCCACCGAGCGGTTGGC
CCAGCTGGTCGTGCAGGTGGCCGGGCGTGCCGGGCGTGGGGAGCAGCCCGGCGAGGTGTTACTGCAGACGCGCCACCCGG
AGCACCCGATGTTGCGCACGCTGCTGGAGCGCGGCTATCCGGCCTTTGCCGAGGCGCACCTGGCCGAGCGCCAGGCGGCC
GGACTGCCGCCCTATGCGGCGCTGGCCCTGTTGCGCGCCGAGTCGGTGCACGCCGACGCCCCCTATCTGTTCCTTGAGTC
GGCGGCGCAGTATGGTCGAAGCCGGCTCGGGAAGGGGGGCGGGGTGGAGCTGCTCGGCCCGGTGCCGGCCCCCATGGAGC
GGCGGGAAGGTCGCCACCGGGCGCAGCTGATGGTCCGTGCCGAGCGGCGCATCGACCTGCAGCGCTTCCTGGTGGCGTGG
AGCGGTGGGCTCGGCAGCCTGGAGGGGGCCCGACAGGTTCGCTGGTCGCTTGACGTGGATCCGGTGGAGATGGTCTGA

Upstream 100 bases:

>100_bases
CATCTCGGTGATGCCCTGCGGGTGGGCGTGGAGGATCGGCTCGACGGCGGCCTGCAATTCGGTGATGGCGGTTTCCGGCT
CCATGGCTGACGACTCTAGC

Downstream 100 bases:

>100_bases
ATCCCGATTTCCGGGACAGGGAGGGTCGAGACGTGTTCGTGATCAGCAAACTGGCCGGTGCCGTGCTGATGCCGCTGAGC
CTGCTGGTGCTGGCCGGTAT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MPPQGGHPQGAGAPPILQVAVPRPLQEALDYRPPPGAWPAEPVGCRVEVPLGRSRAVGIVVAVRHSSDLPASRLRAASAW
VDEQPLLDTELLETLTWAARYYRYPLGTALATALPGPLRQGRPARRAVAWWRLDARLHEDDRAVRRAPRQAELRRRLLAS
GGAAPYALLVAEQPAWQRAGRLLEQAGLLHREYHPAQAEGSADRTPRPGPELDADQQQALECMLAADGYAAFLLEGVTGS
GKTEVYLQTAARALAAGRQVLILVPEIGLAPQFLERLRERLSGSVVVLHSGLSGTDRAEAWLAARDGDAAVILGTRSAIF
TPLPRPGLIVVDEEHDPSLAQQDGFRYSARDLAVLRARTLDVPVVLGSATPSLESLHNARGARYTHLRLPHRAGLARAPS
VQLVDIRSRRLVGGISAPLQEAVRRHLDAGSQVLLFLNRRGYAPVLICHACGWVAGCHRCDARLTYHRSRAQLRCHHCGH
TARVPAACPECGEADLRPLGPGTERLEEALAELFPGVGQLRIDRDTTRRRGALEAQLAAAHRGDAQLLIGTQMLAKGHHL
PAVTLVAVIDVDQGLFGADFRATERLAQLVVQVAGRAGRGEQPGEVLLQTRHPEHPMLRTLLERGYPAFAEAHLAERQAA
GLPPYAALALLRAESVHADAPYLFLESAAQYGRSRLGKGGGVELLGPVPAPMERREGRHRAQLMVRAERRIDLQRFLVAW
SGGLGSLEGARQVRWSLDVDPVEMV

Sequences:

>Translated_745_residues
MPPQGGHPQGAGAPPILQVAVPRPLQEALDYRPPPGAWPAEPVGCRVEVPLGRSRAVGIVVAVRHSSDLPASRLRAASAW
VDEQPLLDTELLETLTWAARYYRYPLGTALATALPGPLRQGRPARRAVAWWRLDARLHEDDRAVRRAPRQAELRRRLLAS
GGAAPYALLVAEQPAWQRAGRLLEQAGLLHREYHPAQAEGSADRTPRPGPELDADQQQALECMLAADGYAAFLLEGVTGS
GKTEVYLQTAARALAAGRQVLILVPEIGLAPQFLERLRERLSGSVVVLHSGLSGTDRAEAWLAARDGDAAVILGTRSAIF
TPLPRPGLIVVDEEHDPSLAQQDGFRYSARDLAVLRARTLDVPVVLGSATPSLESLHNARGARYTHLRLPHRAGLARAPS
VQLVDIRSRRLVGGISAPLQEAVRRHLDAGSQVLLFLNRRGYAPVLICHACGWVAGCHRCDARLTYHRSRAQLRCHHCGH
TARVPAACPECGEADLRPLGPGTERLEEALAELFPGVGQLRIDRDTTRRRGALEAQLAAAHRGDAQLLIGTQMLAKGHHL
PAVTLVAVIDVDQGLFGADFRATERLAQLVVQVAGRAGRGEQPGEVLLQTRHPEHPMLRTLLERGYPAFAEAHLAERQAA
GLPPYAALALLRAESVHADAPYLFLESAAQYGRSRLGKGGGVELLGPVPAPMERREGRHRAQLMVRAERRIDLQRFLVAW
SGGLGSLEGARQVRWSLDVDPVEMV
>Mature_744_residues
PPQGGHPQGAGAPPILQVAVPRPLQEALDYRPPPGAWPAEPVGCRVEVPLGRSRAVGIVVAVRHSSDLPASRLRAASAWV
DEQPLLDTELLETLTWAARYYRYPLGTALATALPGPLRQGRPARRAVAWWRLDARLHEDDRAVRRAPRQAELRRRLLASG
GAAPYALLVAEQPAWQRAGRLLEQAGLLHREYHPAQAEGSADRTPRPGPELDADQQQALECMLAADGYAAFLLEGVTGSG
KTEVYLQTAARALAAGRQVLILVPEIGLAPQFLERLRERLSGSVVVLHSGLSGTDRAEAWLAARDGDAAVILGTRSAIFT
PLPRPGLIVVDEEHDPSLAQQDGFRYSARDLAVLRARTLDVPVVLGSATPSLESLHNARGARYTHLRLPHRAGLARAPSV
QLVDIRSRRLVGGISAPLQEAVRRHLDAGSQVLLFLNRRGYAPVLICHACGWVAGCHRCDARLTYHRSRAQLRCHHCGHT
ARVPAACPECGEADLRPLGPGTERLEEALAELFPGVGQLRIDRDTTRRRGALEAQLAAAHRGDAQLLIGTQMLAKGHHLP
AVTLVAVIDVDQGLFGADFRATERLAQLVVQVAGRAGRGEQPGEVLLQTRHPEHPMLRTLLERGYPAFAEAHLAERQAAG
LPPYAALALLRAESVHADAPYLFLESAAQYGRSRLGKGGGVELLGPVPAPMERREGRHRAQLMVRAERRIDLQRFLVAWS
GGLGSLEGARQVRWSLDVDPVEMV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=735, Percent_Identity=46.3945578231293, Blast_Score=624, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 80869; Mature: 80737

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPQGGHPQGAGAPPILQVAVPRPLQEALDYRPPPGAWPAEPVGCRVEVPLGRSRAVGIV
CCCCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEEE
VAVRHSSDLPASRLRAASAWVDEQPLLDTELLETLTWAARYYRYPLGTALATALPGPLRQ
EEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHC
GRPARRAVAWWRLDARLHEDDRAVRRAPRQAELRRRLLASGGAAPYALLVAEQPAWQRAG
CCCHHHHEEEEEECCHHCCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
RLLEQAGLLHREYHPAQAEGSADRTPRPGPELDADQQQALECMLAADGYAAFLLEGVTGS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCC
GKTEVYLQTAARALAAGRQVLILVPEIGLAPQFLERLRERLSGSVVVLHSGLSGTDRAEA
CCEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHH
WLAARDGDAAVILGTRSAIFTPLPRPGLIVVDEEHDPSLAQQDGFRYSARDLAVLRARTL
EEEECCCCEEEEEECCHHHCCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHC
DVPVVLGSATPSLESLHNARGARYTHLRLPHRAGLARAPSVQLVDIRSRRLVGGISAPLQ
CCEEEECCCCCCHHHHHHCCCCEEEEEECCHHCCCCCCCCEEEEECCCHHHHCCCCHHHH
EAVRRHLDAGSQVLLFLNRRGYAPVLICHACGWVAGCHRCDARLTYHRSRAQLRCHHCGH
HHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
TARVPAACPECGEADLRPLGPGTERLEEALAELFPGVGQLRIDRDTTRRRGALEAQLAAA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHCCCHHHHHHHH
HRGDAQLLIGTQMLAKGHHLPAVTLVAVIDVDQGLFGADFRATERLAQLVVQVAGRAGRG
CCCCCEEEEEHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
EQPGEVLLQTRHPEHPMLRTLLERGYPAFAEAHLAERQAAGLPPYAALALLRAESVHADA
CCCCHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
PYLFLESAAQYGRSRLGKGGGVELLGPVPAPMERREGRHRAQLMVRAERRIDLQRFLVAW
CHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SGGLGSLEGARQVRWSLDVDPVEMV
CCCCCCCCCCHHEEEECCCCHHHCC
>Mature Secondary Structure 
PPQGGHPQGAGAPPILQVAVPRPLQEALDYRPPPGAWPAEPVGCRVEVPLGRSRAVGIV
CCCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEEE
VAVRHSSDLPASRLRAASAWVDEQPLLDTELLETLTWAARYYRYPLGTALATALPGPLRQ
EEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHC
GRPARRAVAWWRLDARLHEDDRAVRRAPRQAELRRRLLASGGAAPYALLVAEQPAWQRAG
CCCHHHHEEEEEECCHHCCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
RLLEQAGLLHREYHPAQAEGSADRTPRPGPELDADQQQALECMLAADGYAAFLLEGVTGS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCC
GKTEVYLQTAARALAAGRQVLILVPEIGLAPQFLERLRERLSGSVVVLHSGLSGTDRAEA
CCEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHH
WLAARDGDAAVILGTRSAIFTPLPRPGLIVVDEEHDPSLAQQDGFRYSARDLAVLRARTL
EEEECCCCEEEEEECCHHHCCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHC
DVPVVLGSATPSLESLHNARGARYTHLRLPHRAGLARAPSVQLVDIRSRRLVGGISAPLQ
CCEEEECCCCCCHHHHHHCCCCEEEEEECCHHCCCCCCCCEEEEECCCHHHHCCCCHHHH
EAVRRHLDAGSQVLLFLNRRGYAPVLICHACGWVAGCHRCDARLTYHRSRAQLRCHHCGH
HHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
TARVPAACPECGEADLRPLGPGTERLEEALAELFPGVGQLRIDRDTTRRRGALEAQLAAA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHCCCHHHHHHHH
HRGDAQLLIGTQMLAKGHHLPAVTLVAVIDVDQGLFGADFRATERLAQLVVQVAGRAGRG
CCCCCEEEEEHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
EQPGEVLLQTRHPEHPMLRTLLERGYPAFAEAHLAERQAAGLPPYAALALLRAESVHADA
CCCCHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
PYLFLESAAQYGRSRLGKGGGVELLGPVPAPMERREGRHRAQLMVRAERRIDLQRFLVAW
CHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SGGLGSLEGARQVRWSLDVDPVEMV
CCCCCCCCCCHHEEEECCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]