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The map label for this gene is murD [H]

Identifier: 121998872

GI number: 121998872

Start: 2297457

End: 2298800

Strand: Reverse

Name: murD [H]

Synonym: Hhal_2093

Alternate gene names: 121998872

Gene position: 2298800-2297457 (Counterclockwise)

Preceding gene: 121998873

Following gene: 121998871

Centisome position: 85.83

GC content: 73.07

Gene sequence:

>1344_bases
ATGACGGCAAGCGACCGCCAGCAAGGGTACACCGCTGTCGCCGGTCTCGGGGCGACGGGGATGGCCTGTGTGCGCCATCT
GCGCGCCCGTGGTGTCGTGGTCGTGGTGGTGGACAGTCGCAGCGAGCCGCCGCGCCTGGCGCAGCTGCAAGCCGAGCACC
CCGAGGTGCGGGTGGTGCTTGGGGGGCTCGACCGCGACACCCTGTTCGGCGCCGAACGCGTGGTGGTCAGCCCCGGTCTC
GACCTGCGCCACGGGGTCTGGACGGAGCTGCGCGCGGCCGGGCGGTCGGTGGTGGGGGAACTCACCCTGTTCGCCGAGGC
GGTGGCGGGTCCGGTGTGCGCCGTAACCGGTTCCAATGGCAAGAGCACGGTGGTCAGCCTGCTCGGCGAGATGGCCCGCG
CTGCCGGGTGGGATGTGGCCGTGGGCGGTAATCTGGGGACGCCCGCCCTGGAGTTGCTCGAGCAGGCGCCCGCCGATGGC
TACCTGATCGAGGTGTCCAGCTTCCAGCTGGAGGCGTGCCCTGGCTTCCGCGCCGATGTGGCGGCGCTGCTCAACGTCAG
CGCCGACCACATGGACCGCTACGACAGCCTCGATGACTACGCGGCGGCCAAGGCACGGGTGCTCGACGGCGCCCGGGTGG
CAGTGCTCAACGCCGAGGATCCCCAGATCGCCGGCCTGGGGCAGCGGGCGGACGCCGTGCGCCATTTCAACCTCGACGGC
CCGGCCGACTACCACCTGCTCGAGCAGGGCGGCCGTGCCTGGCTCGCCGCCGCCGGGCAGCCCCGACAGGCCATCGATGA
GCTGCCGGTACCCGGCCGCGCCCACTGCGCCAACGCCCTGGCCGCCATGGCCCTGGCCGATGCCCTGGGGATTGCCGAGG
TGGCCCAGTGCCGGGCGCTGCAGGCCTTCTCCGGGTTGCCGCATCGCATGGAGTCGGTGGGTGAGTGGCGCGGGGTGCGC
TGGATCAACGACTCCAAGGCGACCAACGTCGGTGCTGCGGTGGCCGCCATCGGCGGCCTGCACCGACCGGTGGTGCTGAT
CGCCGGCGGGCAGGGCAAGGGGGCCGACTTCCGGCCGCTGGCCCAGGCCTGTGCCGAATCGGCGCGTGCGGTGGTCCTCA
TCGGCGAGGACGCAGCGGCCATCGAGGCGGCCATCGCCGGGCGCGTCCCCACCGAGCGGGCCGACAGCATGGTCGGCGCG
GTGGCGGCCGCTGCCCGCTGGGCCGAGCCCGGCGATGCCGTTCTCTTGGCACCGGCATGCGCCAGTTTTGACGCCTTCAG
CAGCTTCGAGGCACGCGGCGATGCGTTCCGTGACGCCGTCCGCGGGGAGGTGGCCCATGGCTGA

Upstream 100 bases:

>100_bases
ACGAACTCAAGGGTTGGCCGGAGCCGCGGGTGATTGTCCGTTTCTGGATCATCACCGTGGTCCTGGTTCTGATCGGTCTG
GCGATGTTGAAGGTGCGCTG

Downstream 100 bases:

>100_bases
TCTCGCAGCCGGTGTCGCCGAGCGCGGTCCGCGACTGTCGCTGTGGTCGAGTCTGGATCAGCGGCTCGTCTGGGTGGTGG
CGGCCACGGCGTTGCTCGGT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTASDRQQGYTAVAGLGATGMACVRHLRARGVVVVVVDSRSEPPRLAQLQAEHPEVRVVLGGLDRDTLFGAERVVVSPGL
DLRHGVWTELRAAGRSVVGELTLFAEAVAGPVCAVTGSNGKSTVVSLLGEMARAAGWDVAVGGNLGTPALELLEQAPADG
YLIEVSSFQLEACPGFRADVAALLNVSADHMDRYDSLDDYAAAKARVLDGARVAVLNAEDPQIAGLGQRADAVRHFNLDG
PADYHLLEQGGRAWLAAAGQPRQAIDELPVPGRAHCANALAAMALADALGIAEVAQCRALQAFSGLPHRMESVGEWRGVR
WINDSKATNVGAAVAAIGGLHRPVVLIAGGQGKGADFRPLAQACAESARAVVLIGEDAAAIEAAIAGRVPTERADSMVGA
VAAAARWAEPGDAVLLAPACASFDAFSSFEARGDAFRDAVRGEVAHG

Sequences:

>Translated_447_residues
MTASDRQQGYTAVAGLGATGMACVRHLRARGVVVVVVDSRSEPPRLAQLQAEHPEVRVVLGGLDRDTLFGAERVVVSPGL
DLRHGVWTELRAAGRSVVGELTLFAEAVAGPVCAVTGSNGKSTVVSLLGEMARAAGWDVAVGGNLGTPALELLEQAPADG
YLIEVSSFQLEACPGFRADVAALLNVSADHMDRYDSLDDYAAAKARVLDGARVAVLNAEDPQIAGLGQRADAVRHFNLDG
PADYHLLEQGGRAWLAAAGQPRQAIDELPVPGRAHCANALAAMALADALGIAEVAQCRALQAFSGLPHRMESVGEWRGVR
WINDSKATNVGAAVAAIGGLHRPVVLIAGGQGKGADFRPLAQACAESARAVVLIGEDAAAIEAAIAGRVPTERADSMVGA
VAAAARWAEPGDAVLLAPACASFDAFSSFEARGDAFRDAVRGEVAHG
>Mature_446_residues
TASDRQQGYTAVAGLGATGMACVRHLRARGVVVVVVDSRSEPPRLAQLQAEHPEVRVVLGGLDRDTLFGAERVVVSPGLD
LRHGVWTELRAAGRSVVGELTLFAEAVAGPVCAVTGSNGKSTVVSLLGEMARAAGWDVAVGGNLGTPALELLEQAPADGY
LIEVSSFQLEACPGFRADVAALLNVSADHMDRYDSLDDYAAAKARVLDGARVAVLNAEDPQIAGLGQRADAVRHFNLDGP
ADYHLLEQGGRAWLAAAGQPRQAIDELPVPGRAHCANALAAMALADALGIAEVAQCRALQAFSGLPHRMESVGEWRGVRW
INDSKATNVGAAVAAIGGLHRPVVLIAGGQGKGADFRPLAQACAESARAVVLIGEDAAAIEAAIAGRVPTERADSMVGAV
AAAARWAEPGDAVLLAPACASFDAFSSFEARGDAFRDAVRGEVAHG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=437, Percent_Identity=43.9359267734554, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46154; Mature: 46022

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASDRQQGYTAVAGLGATGMACVRHLRARGVVVVVVDSRSEPPRLAQLQAEHPEVRVVL
CCCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCCCCEEEEE
GGLDRDTLFGAERVVVSPGLDLRHGVWTELRAAGRSVVGELTLFAEAVAGPVCAVTGSNG
ECCCCHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
KSTVVSLLGEMARAAGWDVAVGGNLGTPALELLEQAPADGYLIEVSSFQLEACPGFRADV
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCCEEEEEECEEEECCCCCHHHH
AALLNVSADHMDRYDSLDDYAAAKARVLDGARVAVLNAEDPQIAGLGQRADAVRHFNLDG
HHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCHHHHHHCCCCCC
PADYHLLEQGGRAWLAAAGQPRQAIDELPVPGRAHCANALAAMALADALGIAEVAQCRAL
CCCHHHHHCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAFSGLPHRMESVGEWRGVRWINDSKATNVGAAVAAIGGLHRPVVLIAGGQGKGADFRPL
HHHCCCCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHH
AQACAESARAVVLIGEDAAAIEAAIAGRVPTERADSMVGAVAAAARWAEPGDAVLLAPAC
HHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCH
ASFDAFSSFEARGDAFRDAVRGEVAHG
HCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TASDRQQGYTAVAGLGATGMACVRHLRARGVVVVVVDSRSEPPRLAQLQAEHPEVRVVL
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCCCCEEEEE
GGLDRDTLFGAERVVVSPGLDLRHGVWTELRAAGRSVVGELTLFAEAVAGPVCAVTGSNG
ECCCCHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
KSTVVSLLGEMARAAGWDVAVGGNLGTPALELLEQAPADGYLIEVSSFQLEACPGFRADV
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCCEEEEEECEEEECCCCCHHHH
AALLNVSADHMDRYDSLDDYAAAKARVLDGARVAVLNAEDPQIAGLGQRADAVRHFNLDG
HHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCEECCCCHHHHHHCCCCCC
PADYHLLEQGGRAWLAAAGQPRQAIDELPVPGRAHCANALAAMALADALGIAEVAQCRAL
CCCHHHHHCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAFSGLPHRMESVGEWRGVRWINDSKATNVGAAVAAIGGLHRPVVLIAGGQGKGADFRPL
HHHCCCCHHHHHHHHHCCEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHH
AQACAESARAVVLIGEDAAAIEAAIAGRVPTERADSMVGAVAAAARWAEPGDAVLLAPAC
HHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCH
ASFDAFSSFEARGDAFRDAVRGEVAHG
HCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA