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The map label for this gene is trpE [H]

Identifier: 121998861

GI number: 121998861

Start: 2285396

End: 2286871

Strand: Reverse

Name: trpE [H]

Synonym: Hhal_2082

Alternate gene names: 121998861

Gene position: 2286871-2285396 (Counterclockwise)

Preceding gene: 121998862

Following gene: 121998860

Centisome position: 85.38

GC content: 70.46

Gene sequence:

>1476_bases
ATGAAAGAGCACGAGTTCCACAGCCTCGCCGAAGCCGGGTACACCCGTATCCCGCTCATCCGCGAGGTCCTGGCCGATCT
CGACACCCCGCTGTCCACCTATCTCAAGCTCGCCCGCGGGCCGCGCTCCTTCCTCTTCGAGTCGGTGGAGGGCGGTGAGA
AGTGGGGCCGCTACTCCATCATCGGGCTGCCGGCCCGGACCGAGATCGCCGTCTACGGGCACCGGGTTGAGGTCCGCCGC
GATGGCGCGCTCGTCGACGAGCAGCAGGTGCGCGATCCGCTGGCCTGGATCGAGGCGTATCAGCACCGTCATCGCGCCGC
GACGCCCTCCGGATTCCCGCAGATGCCGCGGTTCCTGGGGGGGCTGGTGGGGTACTTCGGCTACGACACGGTGCGCTACG
TCGAGCCGCGGCTGGCCGCTAGCGCCCCGGAAGACCCCCTCGGGCTGCCGGACATCTGCCTGGTGGAGGCCGAGGAGGTG
GTCGTCTTCGACAACCTCGCCGGGCGGCTCTATCTGGTGGTCCACTGCGATCCGCGCGAGCCGGACGCCTATGCAGCCGG
CCTGGCACGGCTGGACGAACTGGCCGGGCGGCTGGCCGAGCCCCTGGAGCGCACCCGGCGTCCGGGCGGCGGCGTGGCGG
CGGGGGAGCCCCGCTACAACTTCACCCAGGCCGGGTTCGAGGCCGCGGTGGAGCGGATCCGCGAGTACATCGCCGCCGGC
GACGTGATGCAGGTGGTCCCGTCGCAGCGCATGAGCATGCCGTTCAGCGCGGAGCCCCTCGATCTCTACCGCGCGTTGCG
CTGCACCAACCCCTCGCCCTACATGTACTTCCTCGACCTCGGCGCCTGCCAGGTGGTCGGCTCCTCGCCGGAGATCCTGG
TCCGCCTCGAGGATGGCGAGGTGGCCGTGCGCCCGATTGCCGGGACCCGCAAGCGCGGCGCCACCGAGGCCCGCGACCAG
GAGCTGGAACAGGAGCTGGTCTCCGACCCGAAGGAGATCGCCGAGCACGTGATGCTCATCGACCTCGGGCGCAACGACGT
CGGCCGGGTCGCCGAGACCGGCACGGTGCGCCTGACCGAGCGCATGGTGGTGGAGCGCTACTCCCAGGTCATGCACATCG
TCTCCAACGTGGTCGGGCGGCTGCGACCGGGGCTCGGTCCCATGGACGTGCTGCGGGCCACCTTCCCGGCCGGCACGGTC
TCCGGCGCGCCGAAGATCCGTGCCATGGAGATCATCGACGAGGTCGAGCCGGTCAAGCGCGGTGTCTACGCCGGCGCCGT
CGGCTATCTCTCCTGGTCGGGGAACATGGACACCGCCATCGCGATCCGGACCGCGGTGGTTCACGATGGCCAGGTCCACG
TCCAGGCGGGTGCCGGTGTGGTCGCAGACTCGGTTCCCGAGCTGGAGTGGAAGGAGACCCTGAACAAGGGCCGGGCGCTG
CTGCGCGCCGTCGAGATGGCCGAGGCTGGCTTATGA

Upstream 100 bases:

>100_bases
GGCTACCGCCGCGGGGTGGCGCTGGAAGACCTGGGCGCCGACGCCATCGTGGATGATCTCAGCGGCGTGGTCGCGCTGCT
ACGCGAGGCGGCCTGAGGCC

Downstream 100 bases:

>100_bases
TCCTGATGATCGATAACTACGACTCGTTCACCTGGAATCTCGTCCAGTATCTGGGCGAGCTGGGTGCCCGAGTCGAGGTG
GTGCGCAACGACGAGATCAG

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKEHEFHSLAEAGYTRIPLIREVLADLDTPLSTYLKLARGPRSFLFESVEGGEKWGRYSIIGLPARTEIAVYGHRVEVRR
DGALVDEQQVRDPLAWIEAYQHRHRAATPSGFPQMPRFLGGLVGYFGYDTVRYVEPRLAASAPEDPLGLPDICLVEAEEV
VVFDNLAGRLYLVVHCDPREPDAYAAGLARLDELAGRLAEPLERTRRPGGGVAAGEPRYNFTQAGFEAAVERIREYIAAG
DVMQVVPSQRMSMPFSAEPLDLYRALRCTNPSPYMYFLDLGACQVVGSSPEILVRLEDGEVAVRPIAGTRKRGATEARDQ
ELEQELVSDPKEIAEHVMLIDLGRNDVGRVAETGTVRLTERMVVERYSQVMHIVSNVVGRLRPGLGPMDVLRATFPAGTV
SGAPKIRAMEIIDEVEPVKRGVYAGAVGYLSWSGNMDTAIAIRTAVVHDGQVHVQAGAGVVADSVPELEWKETLNKGRAL
LRAVEMAEAGL

Sequences:

>Translated_491_residues
MKEHEFHSLAEAGYTRIPLIREVLADLDTPLSTYLKLARGPRSFLFESVEGGEKWGRYSIIGLPARTEIAVYGHRVEVRR
DGALVDEQQVRDPLAWIEAYQHRHRAATPSGFPQMPRFLGGLVGYFGYDTVRYVEPRLAASAPEDPLGLPDICLVEAEEV
VVFDNLAGRLYLVVHCDPREPDAYAAGLARLDELAGRLAEPLERTRRPGGGVAAGEPRYNFTQAGFEAAVERIREYIAAG
DVMQVVPSQRMSMPFSAEPLDLYRALRCTNPSPYMYFLDLGACQVVGSSPEILVRLEDGEVAVRPIAGTRKRGATEARDQ
ELEQELVSDPKEIAEHVMLIDLGRNDVGRVAETGTVRLTERMVVERYSQVMHIVSNVVGRLRPGLGPMDVLRATFPAGTV
SGAPKIRAMEIIDEVEPVKRGVYAGAVGYLSWSGNMDTAIAIRTAVVHDGQVHVQAGAGVVADSVPELEWKETLNKGRAL
LRAVEMAEAGL
>Mature_491_residues
MKEHEFHSLAEAGYTRIPLIREVLADLDTPLSTYLKLARGPRSFLFESVEGGEKWGRYSIIGLPARTEIAVYGHRVEVRR
DGALVDEQQVRDPLAWIEAYQHRHRAATPSGFPQMPRFLGGLVGYFGYDTVRYVEPRLAASAPEDPLGLPDICLVEAEEV
VVFDNLAGRLYLVVHCDPREPDAYAAGLARLDELAGRLAEPLERTRRPGGGVAAGEPRYNFTQAGFEAAVERIREYIAAG
DVMQVVPSQRMSMPFSAEPLDLYRALRCTNPSPYMYFLDLGACQVVGSSPEILVRLEDGEVAVRPIAGTRKRGATEARDQ
ELEQELVSDPKEIAEHVMLIDLGRNDVGRVAETGTVRLTERMVVERYSQVMHIVSNVVGRLRPGLGPMDVLRATFPAGTV
SGAPKIRAMEIIDEVEPVKRGVYAGAVGYLSWSGNMDTAIAIRTAVVHDGQVHVQAGAGVVADSVPELEWKETLNKGRAL
LRAVEMAEAGL

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=383, Percent_Identity=40.7310704960835, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788114, Length=478, Percent_Identity=31.5899581589958, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI87082077, Length=285, Percent_Identity=27.0175438596491, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786809, Length=264, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=479, Percent_Identity=38.2045929018789, Blast_Score=301, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6324361, Length=406, Percent_Identity=25.8620689655172, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 53936; Mature: 53936

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEHEFHSLAEAGYTRIPLIREVLADLDTPLSTYLKLARGPRSFLFESVEGGEKWGRYSI
CCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCEEE
IGLPARTEIAVYGHRVEVRRDGALVDEQQVRDPLAWIEAYQHRHRAATPSGFPQMPRFLG
EECCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GLVGYFGYDTVRYVEPRLAASAPEDPLGLPDICLVEAEEVVVFDNLAGRLYLVVHCDPRE
HHHHHHCCCHHHHHCCHHHCCCCCCCCCCCCEEEEECCCEEEEECCCCEEEEEEEECCCC
PDAYAAGLARLDELAGRLAEPLERTRRPGGGVAAGEPRYNFTQAGFEAAVERIREYIAAG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
DVMQVVPSQRMSMPFSAEPLDLYRALRCTNPSPYMYFLDLGACQVVGSSPEILVRLEDGE
HHHHHCCHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCEEEEEEECCC
VAVRPIAGTRKRGATEARDQELEQELVSDPKEIAEHVMLIDLGRNDVGRVAETGTVRLTE
EEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCHHHCCCCCHHHH
RMVVERYSQVMHIVSNVVGRLRPGLGPMDVLRATFPAGTVSGAPKIRAMEIIDEVEPVKR
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GVYAGAVGYLSWSGNMDTAIAIRTAVVHDGQVHVQAGAGVVADSVPELEWKETLNKGRAL
HHHHCEEEEEEECCCCCCEEEEEEEEEECCEEEEEECCCCHHCCCCCCHHHHHHHHHHHH
LRAVEMAEAGL
HHHHHHHHCCC
>Mature Secondary Structure
MKEHEFHSLAEAGYTRIPLIREVLADLDTPLSTYLKLARGPRSFLFESVEGGEKWGRYSI
CCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCEEE
IGLPARTEIAVYGHRVEVRRDGALVDEQQVRDPLAWIEAYQHRHRAATPSGFPQMPRFLG
EECCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GLVGYFGYDTVRYVEPRLAASAPEDPLGLPDICLVEAEEVVVFDNLAGRLYLVVHCDPRE
HHHHHHCCCHHHHHCCHHHCCCCCCCCCCCCEEEEECCCEEEEECCCCEEEEEEEECCCC
PDAYAAGLARLDELAGRLAEPLERTRRPGGGVAAGEPRYNFTQAGFEAAVERIREYIAAG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
DVMQVVPSQRMSMPFSAEPLDLYRALRCTNPSPYMYFLDLGACQVVGSSPEILVRLEDGE
HHHHHCCHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCEEEEEEECCC
VAVRPIAGTRKRGATEARDQELEQELVSDPKEIAEHVMLIDLGRNDVGRVAETGTVRLTE
EEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCHHHCCCCCHHHH
RMVVERYSQVMHIVSNVVGRLRPGLGPMDVLRATFPAGTVSGAPKIRAMEIIDEVEPVKR
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GVYAGAVGYLSWSGNMDTAIAIRTAVVHDGQVHVQAGAGVVADSVPELEWKETLNKGRAL
HHHHCEEEEEEECCCCCCEEEEEEEEEECCEEEEEECCCCHHCCCCCCHHHHHHHHHHHH
LRAVEMAEAGL
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2404959 [H]