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The map label for this gene is cobB [H]

Identifier: 121998688

GI number: 121998688

Start: 2104628

End: 2105908

Strand: Reverse

Name: cobB [H]

Synonym: Hhal_1909

Alternate gene names: 121998688

Gene position: 2105908-2104628 (Counterclockwise)

Preceding gene: 121998689

Following gene: 121998687

Centisome position: 78.62

GC content: 73.15

Gene sequence:

>1281_bases
GTGGGCACGCCCGCGCTGATGCTCGCCGCGCCCGGCTCGGGGCAGGGCAAGTCGATGATCACGGCAGCCCTGGCCCGGCT
GCACCGCAACGCCGGACGGCGGGTGCGGGTGTTCAAGCACGGGCCGGACTACCTGGATCCGATGATCCAGGAACGGGCCT
CCGGGGCTCCGGTGTATCAGCTCCACCCGTGGATGACCGGGGCTGCCGAGTGCCGCGACCGCCTGGCCGCCGCGGCCCGC
GAGGCCGACGTGGTGCTGGTGGAGGGGTCCATGGGGCTGTTCGACGGGGACCCTTCCAGTGCCGACTTGGCGGCGTTGGC
CGGTCTGCCGGTGGCGCTGGTGATCGACGCCCGCGCCATGGCCGAGACCTTCGGCGCCGTGGTCGGTGGGTTGGCCCGGC
ACCGCGCGGATGTGCAGGTGATCGGCGCCATCGCCAACCGGGTGGGCAGCCCTGGCCACGCGCGCATGCTCGCCGGCAGT
CTGCCCGAGGGGGTGGCCTGGTTGGGGGCGGTGCCCCGCGACGACGCCATGCATCTGCCCGATCGCCATCTGGGGCTGGT
CCAGGCCGACGAGGTGGCCGACCTGGATGCGCGTCTGGATGCGGCAGCGGCCGTGCTGGCCGATGCCGGGCTCGGTGCAC
TGCCCCCGGCGGTCACCCTGGAGGCCGATCCGTTGCCGCCACCGCCGCGACGGCTGGTCGGTCAGCGGATCGCCGTGGCC
CGGGATGCGGCCTTCGCCTTCATCTACCGTGGCAATCTGGAGCTGCTCGAAGCGATGGGGGCGGAGGTGGTGCCGTTCTC
GCCGCTGCACGACACTGCGCTGCCGGCCTGTGACGCCCTGTGGCTACCCGGCGGTTACCCGGAGCTCCACGCCGCCACCC
TGGCTGCCAACAGCGGGCTGTGGGCGGCGATCCACGCGCACCACCAGGCCGGCAAGCCGATCCTCGCCGAGTGCGGCGGG
CTGATGTACTGCGCCGAGTCCCTGGTGGACGGCGAGGGGGTGACGCACCCGATGCTTGGGCTGTTGCCCGGGCGCGTCGA
GATGGCCGGTCGCCTGACCGGGATCGGCCTGCAGAGCCTGGCGCGACCGGACGGCGAGCTGCGCGGCCACACCTTCCACC
ACTCCCGGCTGGAGACCGCCCTGGAGCCGGCGGAGTCTACCACCCGGCGCAGTGGCAGCCCGGCCGAGGCGGTCTATCGG
GTCGGGTCGCTGACGGCCACCTACTTCCACGCCTATTTCCCCTCGGCTCCCGAGGTCGTCGCCGGCCTGTTCAGTCGTTA
G

Upstream 100 bases:

>100_bases
TCGGCCACTCGCGGCTGCGCCGGTTCTTTGTCGGCAGTACCACGACGCACATGATCATGCGTTCGAGCACGCCGCTGCTC
ATCCTGCGTTAGGAGCGGCC

Downstream 100 bases:

>100_bases
GCTACGTCGATCTGCCGCCGGTGCCCGGTGGTCCCCCGAAGGTAAGGAGGTGAGAGAAGCGATGCGTGAAGATGCGAAGG
ATCCGCAACGCCACGCCGCG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MGTPALMLAAPGSGQGKSMITAALARLHRNAGRRVRVFKHGPDYLDPMIQERASGAPVYQLHPWMTGAAECRDRLAAAAR
EADVVLVEGSMGLFDGDPSSADLAALAGLPVALVIDARAMAETFGAVVGGLARHRADVQVIGAIANRVGSPGHARMLAGS
LPEGVAWLGAVPRDDAMHLPDRHLGLVQADEVADLDARLDAAAAVLADAGLGALPPAVTLEADPLPPPPRRLVGQRIAVA
RDAAFAFIYRGNLELLEAMGAEVVPFSPLHDTALPACDALWLPGGYPELHAATLAANSGLWAAIHAHHQAGKPILAECGG
LMYCAESLVDGEGVTHPMLGLLPGRVEMAGRLTGIGLQSLARPDGELRGHTFHHSRLETALEPAESTTRRSGSPAEAVYR
VGSLTATYFHAYFPSAPEVVAGLFSR

Sequences:

>Translated_426_residues
MGTPALMLAAPGSGQGKSMITAALARLHRNAGRRVRVFKHGPDYLDPMIQERASGAPVYQLHPWMTGAAECRDRLAAAAR
EADVVLVEGSMGLFDGDPSSADLAALAGLPVALVIDARAMAETFGAVVGGLARHRADVQVIGAIANRVGSPGHARMLAGS
LPEGVAWLGAVPRDDAMHLPDRHLGLVQADEVADLDARLDAAAAVLADAGLGALPPAVTLEADPLPPPPRRLVGQRIAVA
RDAAFAFIYRGNLELLEAMGAEVVPFSPLHDTALPACDALWLPGGYPELHAATLAANSGLWAAIHAHHQAGKPILAECGG
LMYCAESLVDGEGVTHPMLGLLPGRVEMAGRLTGIGLQSLARPDGELRGHTFHHSRLETALEPAESTTRRSGSPAEAVYR
VGSLTATYFHAYFPSAPEVVAGLFSR
>Mature_425_residues
GTPALMLAAPGSGQGKSMITAALARLHRNAGRRVRVFKHGPDYLDPMIQERASGAPVYQLHPWMTGAAECRDRLAAAARE
ADVVLVEGSMGLFDGDPSSADLAALAGLPVALVIDARAMAETFGAVVGGLARHRADVQVIGAIANRVGSPGHARMLAGSL
PEGVAWLGAVPRDDAMHLPDRHLGLVQADEVADLDARLDAAAAVLADAGLGALPPAVTLEADPLPPPPRRLVGQRIAVAR
DAAFAFIYRGNLELLEAMGAEVVPFSPLHDTALPACDALWLPGGYPELHAATLAANSGLWAAIHAHHQAGKPILAECGGL
MYCAESLVDGEGVTHPMLGLLPGRVEMAGRLTGIGLQSLARPDGELRGHTFHHSRLETALEPAESTTRRSGSPAEAVYRV
GSLTATYFHAYFPSAPEVVAGLFSR

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 44423; Mature: 44292

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTPALMLAAPGSGQGKSMITAALARLHRNAGRRVRVFKHGPDYLDPMIQERASGAPVYQ
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEE
LHPWMTGAAECRDRLAAAAREADVVLVEGSMGLFDGDPSSADLAALAGLPVALVIDARAM
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEECHHHH
AETFGAVVGGLARHRADVQVIGAIANRVGSPGHARMLAGSLPEGVAWLGAVPRDDAMHLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCCCCCCCCC
DRHLGLVQADEVADLDARLDAAAAVLADAGLGALPPAVTLEADPLPPPPRRLVGQRIAVA
CHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
RDAAFAFIYRGNLELLEAMGAEVVPFSPLHDTALPACDALWLPGGYPELHAATLAANSGL
HHCEEEEEEECCHHHHHHCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHEECCCCC
WAAIHAHHQAGKPILAECGGLMYCAESLVDGEGVTHPMLGLLPGRVEMAGRLTGIGLQSL
EEEEECHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHCCCHHHH
ARPDGELRGHTFHHSRLETALEPAESTTRRSGSPAEAVYRVGSLTATYFHAYFPSAPEVV
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
AGLFSR
HHHHCC
>Mature Secondary Structure 
GTPALMLAAPGSGQGKSMITAALARLHRNAGRRVRVFKHGPDYLDPMIQERASGAPVYQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEEE
LHPWMTGAAECRDRLAAAAREADVVLVEGSMGLFDGDPSSADLAALAGLPVALVIDARAM
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEECHHHH
AETFGAVVGGLARHRADVQVIGAIANRVGSPGHARMLAGSLPEGVAWLGAVPRDDAMHLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHCCCCCCCCCCCC
DRHLGLVQADEVADLDARLDAAAAVLADAGLGALPPAVTLEADPLPPPPRRLVGQRIAVA
CHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHH
RDAAFAFIYRGNLELLEAMGAEVVPFSPLHDTALPACDALWLPGGYPELHAATLAANSGL
HHCEEEEEEECCHHHHHHCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHEECCCCC
WAAIHAHHQAGKPILAECGGLMYCAESLVDGEGVTHPMLGLLPGRVEMAGRLTGIGLQSL
EEEEECHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHCCCHHHH
ARPDGELRGHTFHHSRLETALEPAESTTRRSGSPAEAVYRVGSLTATYFHAYFPSAPEVV
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
AGLFSR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]