Click here to switch to the map view.

The map label for this gene is proB

Identifier: 121998624

GI number: 121998624

Start: 2017671

End: 2018801

Strand: Reverse

Name: proB

Synonym: Hhal_1845

Alternate gene names: 121998624

Gene position: 2018801-2017671 (Counterclockwise)

Preceding gene: 121998625

Following gene: 121998623

Centisome position: 75.37

GC content: 68.97

Gene sequence:

>1131_bases
GTGAGACGGCGTGAGGCGCTGCGCCAGGCGCAGCGTTGGGTGATCAAGGTCGGCAGCGCCCTGATTACCGATGACGGGCG
AGGGTTGGCCCACGAGCAGATGAGTGCTTGGGCGGACCAGGTGGCGGCCTTGCGCAAGGCGGGGCGTCAGGTCACCCTGG
TCTCATCCGGAGCGGTGGCCGAGGGCATGCAGCGCCTCGGTTGGCGCTCCCGGCCGCGTGCGCTCTATCAGCTGCAGGCC
GCGGCTGGGGTGGGGCAGAGCGGCCTGGTGCACGCCTGGTCCGACGGGCTGGCCCGGCACCGACTGCAGACTGCCCAGGT
GCTACTGACCCACGATGACCTCTCCGACCGGCGGCGTTACCTCAATGCCCGCAGCGCTCTGCGCGAGATGCTGCGCCTGG
GGGTGGTGCCGGTGGTCAATGAGAACGACACGGTGGTCACCGACGAGATCCGGTTTGGTGACAACGATACGCTGGCTGCC
CTGGTCGCCAATCTGGTGGAGGCCGAGGCGCTGGTGGTCTTGACCGATCAGCCCGGCTTGATGGACCGCGATCCGCGCGA
GCACCCCGACGCCGTGCTGCTCGATGAGGTTCGCGCCGGGGACCCGGAGCTCGAGCGGTTGTGCGGCAGTCCTGCCGGGG
TGCTGGGTCGAGGCGGTATGCTTACGAAGGTCCGCGGTGCTGAGCGGGCCGCGCGTTCCGGTACCTACACAGTGGTTGCC
AGTGGCCGCGAGGGGCGGGTGATCCAGCGCCTGGCTGATGCCGAGCCGGGGCTGGGGACCCTCTTCGTGCCGGATCAGGA
GCCCCTGGCGGCACGCAAGCAGTGGCTGGCCAGCCATCTGCAAACCCGTGGTGCCCTGAGCCTCGACGAAGGGGCGGCGC
GTGCCCTGCGCGAGTCCGGTAAGAGCCTGCTACCGGTGGGCGTGGTGGCCGTGGAGGGGGGGTTCTCCCGTGGCGAGATG
GTTGTCTGCCGGGATCCGGCGGGTATTGAGGTCGCGCGCGGGTTGGTGAACTACGCCGCCGAAGAGGCCCGGTTAATCTG
CGGCCGTGCCAGTCGGGACATCGAAACGACGCTCGGCTACGTCGATGAGCCGGAGTTGATCCATCGCGATAACATGGTGG
TCACGGTGTAG

Upstream 100 bases:

>100_bases
GGCCCGGTCTACGGCATCTCCGGGCTCACTGGGGAGGGCGTGGACCGGCTCTGCGGGGACATCATGAACGATCTGGAGGC
GCGACGCAGGGAGGAGGACG

Downstream 100 bases:

>100_bases
GTCATGTGCCGAACTCCCCACCGCGTCTTTGCCTTGCTGACCGCCGGGGTGGCTCTGGCCTCGTTGCTGACCGCCTGCAG
TGGCTGGCCGTTGACCCCGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRRREALRQAQRWVIKVGSALITDDGRGLAHEQMSAWADQVAALRKAGRQVTLVSSGAVAEGMQRLGWRSRPRALYQLQA
AAGVGQSGLVHAWSDGLARHRLQTAQVLLTHDDLSDRRRYLNARSALREMLRLGVVPVVNENDTVVTDEIRFGDNDTLAA
LVANLVEAEALVVLTDQPGLMDRDPREHPDAVLLDEVRAGDPELERLCGSPAGVLGRGGMLTKVRGAERAARSGTYTVVA
SGREGRVIQRLADAEPGLGTLFVPDQEPLAARKQWLASHLQTRGALSLDEGAARALRESGKSLLPVGVVAVEGGFSRGEM
VVCRDPAGIEVARGLVNYAAEEARLICGRASRDIETTLGYVDEPELIHRDNMVVTV

Sequences:

>Translated_376_residues
MRRREALRQAQRWVIKVGSALITDDGRGLAHEQMSAWADQVAALRKAGRQVTLVSSGAVAEGMQRLGWRSRPRALYQLQA
AAGVGQSGLVHAWSDGLARHRLQTAQVLLTHDDLSDRRRYLNARSALREMLRLGVVPVVNENDTVVTDEIRFGDNDTLAA
LVANLVEAEALVVLTDQPGLMDRDPREHPDAVLLDEVRAGDPELERLCGSPAGVLGRGGMLTKVRGAERAARSGTYTVVA
SGREGRVIQRLADAEPGLGTLFVPDQEPLAARKQWLASHLQTRGALSLDEGAARALRESGKSLLPVGVVAVEGGFSRGEM
VVCRDPAGIEVARGLVNYAAEEARLICGRASRDIETTLGYVDEPELIHRDNMVVTV
>Mature_376_residues
MRRREALRQAQRWVIKVGSALITDDGRGLAHEQMSAWADQVAALRKAGRQVTLVSSGAVAEGMQRLGWRSRPRALYQLQA
AAGVGQSGLVHAWSDGLARHRLQTAQVLLTHDDLSDRRRYLNARSALREMLRLGVVPVVNENDTVVTDEIRFGDNDTLAA
LVANLVEAEALVVLTDQPGLMDRDPREHPDAVLLDEVRAGDPELERLCGSPAGVLGRGGMLTKVRGAERAARSGTYTVVA
SGREGRVIQRLADAEPGLGTLFVPDQEPLAARKQWLASHLQTRGALSLDEGAARALRESGKSLLPVGVVAVEGGFSRGEM
VVCRDPAGIEVARGLVNYAAEEARLICGRASRDIETTLGYVDEPELIHRDNMVVTV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=34.9152542372881, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=35.1535836177474, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=39.2370572207085, Blast_Score=255, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17569969, Length=281, Percent_Identity=32.7402135231317, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17569967, Length=281, Percent_Identity=32.7402135231317, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=412, Percent_Identity=32.5242718446602, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=32.2966507177034, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI21357643, Length=299, Percent_Identity=31.438127090301, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_HALHL (A1WY47)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003411.1
- ProteinModelPortal:   A1WY47
- STRING:   A1WY47
- GeneID:   4711344
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1845
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   A1WY47
- ProtClustDB:   PRK05429
- BioCyc:   HHAL349124:HHAL_1845-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40685; Mature: 40685

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRREALRQAQRWVIKVGSALITDDGRGLAHEQMSAWADQVAALRKAGRQVTLVSSGAVA
CCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
EGMQRLGWRSRPRALYQLQAAAGVGQSGLVHAWSDGLARHRLQTAQVLLTHDDLSDRRRY
HHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHEEEECCCHHHHHHH
LNARSALREMLRLGVVPVVNENDTVVTDEIRFGDNDTLAALVANLVEAEALVVLTDQPGL
HHHHHHHHHHHHHCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCC
MDRDPREHPDAVLLDEVRAGDPELERLCGSPAGVLGRGGMLTKVRGAERAARSGTYTVVA
CCCCCCCCCCCEEEHHHCCCCHHHHHHCCCCCCHHCCCCCEEEECCHHHHHCCCCEEEEE
SGREGRVIQRLADAEPGLGTLFVPDQEPLAARKQWLASHLQTRGALSLDEGAARALRESG
CCCCCHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
KSLLPVGVVAVEGGFSRGEMVVCRDPAGIEVARGLVNYAAEEARLICGRASRDIETTLGY
CCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCC
VDEPELIHRDNMVVTV
CCCCCCEECCCEEEEC
>Mature Secondary Structure
MRRREALRQAQRWVIKVGSALITDDGRGLAHEQMSAWADQVAALRKAGRQVTLVSSGAVA
CCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
EGMQRLGWRSRPRALYQLQAAAGVGQSGLVHAWSDGLARHRLQTAQVLLTHDDLSDRRRY
HHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHEEEECCCHHHHHHH
LNARSALREMLRLGVVPVVNENDTVVTDEIRFGDNDTLAALVANLVEAEALVVLTDQPGL
HHHHHHHHHHHHHCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCC
MDRDPREHPDAVLLDEVRAGDPELERLCGSPAGVLGRGGMLTKVRGAERAARSGTYTVVA
CCCCCCCCCCCEEEHHHCCCCHHHHHHCCCCCCHHCCCCCEEEECCHHHHHCCCCEEEEE
SGREGRVIQRLADAEPGLGTLFVPDQEPLAARKQWLASHLQTRGALSLDEGAARALRESG
CCCCCHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
KSLLPVGVVAVEGGFSRGEMVVCRDPAGIEVARGLVNYAAEEARLICGRASRDIETTLGY
CCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCC
VDEPELIHRDNMVVTV
CCCCCCEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA