Click here to switch to the map view.

The map label for this gene is rlmN

Identifier: 121998570

GI number: 121998570

Start: 1964450

End: 1965529

Strand: Reverse

Name: rlmN

Synonym: Hhal_1791

Alternate gene names: 121998570

Gene position: 1965529-1964450 (Counterclockwise)

Preceding gene: 121998571

Following gene: 121998569

Centisome position: 73.38

GC content: 66.94

Gene sequence:

>1080_bases
GTGCATGCCGAGCGCCGGTCGGGTCCGGTCGAGCTGCTAGGGCTCGATCGGCCCCGGTTGGCGGCCTTCTTCGATACCCT
GGGCGAGAAGCGGTTTCGCGCCCGGCAGATCATGCAGTGGCTGCATCAACGCCACGTGTACGACTTCGACGAGATGACCG
ACCTCAGTAAGGCGTTGCGCCAGCGGCTGCGTGAGCACGCCCGGGTCGGTCTCCCCGAGGTGGCTGCGGATCAGCAGGCC
AGCGACGGCACCCGCAAGTGGGTGGTGCGCCTGGCCGACGGCAACTGTGTCGAGGCCGTCTACATCCCCGAGCCCAAACG
CGGGACGCTGTGCATCTCCTCCCAGGCCGGGTGCCCGATGGGTTGCACCTTTTGCGCCACCGGCGAGGGCGGTTTCTCGC
GGAACCTCACCGCTGCCGAGATCGTGGGTCAGGTCCATGTGGCGCGCCAGCACCTTCCGGAGGGGGCGATTACCAACATC
GTCTTCATGGGAATGGGCGAGCCGTTGCTCAACTTCGACCCGGTCATTTCGGCCTCACGCGTGTTCACCGACGACTACGG
GTTCGTGCTCTCCAAGCGTCGGGTGACCATCTCGACCTCGGGCGTCGTCCACGCCATCGAGCGCATGCAACGCGTCACGG
ATGTCAGCCTGGCGGTATCGCTGCACGCGCCCAACAACGAACTGCGCAACCAGTTGGTCCCGCTCAACCGCAAGAATCCC
CTCGAGCGCCTGCTGCCGGCCTGCCACGCCTATATCGCGGAAAAGCCGCATCGGCGGATCACCTGGGAGTACGTCATGCT
CGATGGGGTGAACGATCAGGACGAGCACGCCCGCGAGCTGCTCCAGCGGCTGCGCGGGATCCCGTCGAAGGTGAACCTGA
TTCCGTTCAACCCCTATCCCGGGGCGCGCTACGGCCGCACGCCGGACCGCCAGGTGCGCCGGTTTGCGGATCGGCTGCTC
GAGCACGGACTAACCGCGACCATCCGCGAGACCCGCGGGGATGACATCGACGGCGCCTGTGGACAACTCGTCGGCGAGAT
TCGCGACGCCCGGGCCAGCAAGGTGGCGAGACCGGCGTGA

Upstream 100 bases:

>100_bases
GCCAACGCGGTTCACGGATCGGATAGCCCCGAGACGGCGGAAAACGAGATCGAGTTCTTCTTTAAGGCCGATGAGATCTG
CTCCCGCTGACCACGAGGCG

Downstream 100 bases:

>100_bases
GGCGAGCGGGGCCCATGCTGGCGGTCCTGATGGTTGCAGGTTGCGCGACCGGGCCCGAGGTGCCTGACGATCGGCGCGCA
GCCGATAGCCACGCTGGAGC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MHAERRSGPVELLGLDRPRLAAFFDTLGEKRFRARQIMQWLHQRHVYDFDEMTDLSKALRQRLREHARVGLPEVAADQQA
SDGTRKWVVRLADGNCVEAVYIPEPKRGTLCISSQAGCPMGCTFCATGEGGFSRNLTAAEIVGQVHVARQHLPEGAITNI
VFMGMGEPLLNFDPVISASRVFTDDYGFVLSKRRVTISTSGVVHAIERMQRVTDVSLAVSLHAPNNELRNQLVPLNRKNP
LERLLPACHAYIAEKPHRRITWEYVMLDGVNDQDEHARELLQRLRGIPSKVNLIPFNPYPGARYGRTPDRQVRRFADRLL
EHGLTATIRETRGDDIDGACGQLVGEIRDARASKVARPA

Sequences:

>Translated_359_residues
MHAERRSGPVELLGLDRPRLAAFFDTLGEKRFRARQIMQWLHQRHVYDFDEMTDLSKALRQRLREHARVGLPEVAADQQA
SDGTRKWVVRLADGNCVEAVYIPEPKRGTLCISSQAGCPMGCTFCATGEGGFSRNLTAAEIVGQVHVARQHLPEGAITNI
VFMGMGEPLLNFDPVISASRVFTDDYGFVLSKRRVTISTSGVVHAIERMQRVTDVSLAVSLHAPNNELRNQLVPLNRKNP
LERLLPACHAYIAEKPHRRITWEYVMLDGVNDQDEHARELLQRLRGIPSKVNLIPFNPYPGARYGRTPDRQVRRFADRLL
EHGLTATIRETRGDDIDGACGQLVGEIRDARASKVARPA
>Mature_359_residues
MHAERRSGPVELLGLDRPRLAAFFDTLGEKRFRARQIMQWLHQRHVYDFDEMTDLSKALRQRLREHARVGLPEVAADQQA
SDGTRKWVVRLADGNCVEAVYIPEPKRGTLCISSQAGCPMGCTFCATGEGGFSRNLTAAEIVGQVHVARQHLPEGAITNI
VFMGMGEPLLNFDPVISASRVFTDDYGFVLSKRRVTISTSGVVHAIERMQRVTDVSLAVSLHAPNNELRNQLVPLNRKNP
LERLLPACHAYIAEKPHRRITWEYVMLDGVNDQDEHARELLQRLRGIPSKVNLIPFNPYPGARYGRTPDRQVRRFADRLL
EHGLTATIRETRGDDIDGACGQLVGEIRDARASKVARPA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=49.4318181818182, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_HALHL (A1WXZ3)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003357.1
- ProteinModelPortal:   A1WXZ3
- STRING:   A1WXZ3
- GeneID:   4710980
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1791
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   A1WXZ3
- BioCyc:   HHAL349124:HHAL_1791-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40296; Mature: 40296

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAERRSGPVELLGLDRPRLAAFFDTLGEKRFRARQIMQWLHQRHVYDFDEMTDLSKALR
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QRLREHARVGLPEVAADQQASDGTRKWVVRLADGNCVEAVYIPEPKRGTLCISSQAGCPM
HHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEECCCCCCC
GCTFCATGEGGFSRNLTAAEIVGQVHVARQHLPEGAITNIVFMGMGEPLLNFDPVISASR
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCCCCCHHH
VFTDDYGFVLSKRRVTISTSGVVHAIERMQRVTDVSLAVSLHAPNNELRNQLVPLNRKNP
HEECCCCCEEECCEEEEECHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHCCCCCCCCH
LERLLPACHAYIAEKPHRRITWEYVMLDGVNDQDEHARELLQRLRGIPSKVNLIPFNPYP
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
GARYGRTPDRQVRRFADRLLEHGLTATIRETRGDDIDGACGQLVGEIRDARASKVARPA
CCCCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHAERRSGPVELLGLDRPRLAAFFDTLGEKRFRARQIMQWLHQRHVYDFDEMTDLSKALR
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QRLREHARVGLPEVAADQQASDGTRKWVVRLADGNCVEAVYIPEPKRGTLCISSQAGCPM
HHHHHHHHCCCCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEECCCCCCC
GCTFCATGEGGFSRNLTAAEIVGQVHVARQHLPEGAITNIVFMGMGEPLLNFDPVISASR
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCCCCCCCCCHHH
VFTDDYGFVLSKRRVTISTSGVVHAIERMQRVTDVSLAVSLHAPNNELRNQLVPLNRKNP
HEECCCCCEEECCEEEEECHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHCCCCCCCCH
LERLLPACHAYIAEKPHRRITWEYVMLDGVNDQDEHARELLQRLRGIPSKVNLIPFNPYP
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
GARYGRTPDRQVRRFADRLLEHGLTATIRETRGDDIDGACGQLVGEIRDARASKVARPA
CCCCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA