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The map label for this gene is nusA

Identifier: 121998529

GI number: 121998529

Start: 1922385

End: 1923890

Strand: Reverse

Name: nusA

Synonym: Hhal_1750

Alternate gene names: 121998529

Gene position: 1923890-1922385 (Counterclockwise)

Preceding gene: 121998530

Following gene: 121998528

Centisome position: 71.83

GC content: 65.87

Gene sequence:

>1506_bases
ATGAGTAAAGAAATCCTGTTGGTCGTGGAGGCCACCTCCAATGAGAAGGGTGTGGACCGGGAGGTGATCTTCGAGGCCAT
TGAGGCGGCCCTGGCCTCGGCCACGCGCAAGCGCCATCTGGAGGACATCGACGCCCGCGTTGCGGTCGATCGGCAGAGTG
GCGATTACGCCACCTATCGGCGCTGGGAGGTGGTCCCCGACGAAGAGTCGGTGGAGGAGCCGCAGCGCCAGATCAGCCTG
GAGCGTGCCCGGGAGCGTGATGAGAACGCCGAAGTGGGCGGGTATGTCGAGGAGCCCATCGAATCGGTCGACTTCGGCCG
CATCGCCGCGCAGACTGCCAAGCAGGTCATCGTGCAGAAGGTGCGCGAGGCCGAGCGGGCGCAGATCGTTGACGCTTACC
AGCACCGTATCGGCGAGTTGGTCAACGGCTCGGTCAAGCGTATGGAGCGCGGTAGCGCCATCATCGATCTGGGCGAGAAC
ACCGAGGCTCTAGTCTCCCGCGAAGATCTGATCCCGCGGGAGGCTGTGCGACCCAACGACCGTATTCGCGGCTACCTGCG
TGACGTCCGCTCAGAGCCGCGGGGGCCGCAACTGTTCGTCAGCCGCACGGCCAATGAGTTTCTGGTCGAGCTCTTCAAGA
TCGAGGTGCCCGAAGTCGGCCAGGGGTTGATCGAGATCCTGGGCGCCGCCCGCGATCCGGGGATGCGCGCCAAGATCTCG
GTGCGGGCTCTGGATCCGCGCATCGATCCGGTCGGGGCCTGCGTGGGTATGCGCGGCTCGCGCGTGCAGGCAGTCTCCAA
CGAGCTCAGCGGCGAGCGGATCGATATTATCCCCTGGGATGAGAACCCGGCTCAGTTTGTCATCAACGCGTTGGCGCCGG
CCGAGGTCGAGTCCATCGTGGTCGATGAGGACCGCGGTGCCATGGATGTCGCCGTCGCCGAGGAGCAACTCTCCCAGGCG
ATCGGTCGTGGTGGGCAGAACGTGCGGCTGGCCAGCGAGCTCACCGGCTGGGAACTCAACGTGATGACGGCCGAGGAGGC
CGAGGCGAAGAGCGAGCGCGAAGCCGGTGAGCTGGTGCAGTTGTTCGTCGAGCACCTCGACGTCGATGAAGATGTTGCCG
GCGTGCTGGTCCAGGAAGGCTTTTCCAGCATCGAGGAGGTGGCCTACGTGCCCACCGCCGAGCTGCTGGAGATCGAGGAG
TTCGACGAGGACATCGTCGAGGCCCTGCGCAGTCGCGCCCGGGATGTGCTCCTGGCCCAGGCGATTGCCGAGGAGGCCAA
CGAGAACCAGCCCAGCGAGGAGCTGCTGGCCCTTGAGGGGATGGATGAGCAGACGGCGAAAGCCCTGGCCGAGCGCGGTG
TCGCCACGGTCGAGGACCTGGCCGATCAGTCCGTGGACGACCTGATGGAGGTCGAAGGCATGGACGAGGATCGGGCCGGA
CAGCTGATCATGAAGGCCCGCGAGCCGTGGTTCGCAGCCAGTGAGGGCGGCGAGCGCGCCGACTGA

Upstream 100 bases:

>100_bases
GGGGTCGAGCTGCGCATCCCCCTGGAGCTGATCGACCGGGCGCGGCTGGATCTCGAGCCGTGAAGGGCGTCGGCCGCAGC
AGAACAGCGGAGTCGGAGGC

Downstream 100 bases:

>100_bases
TAAAGAGGGGTCACGACGAGGTCTCTGACTTGGGCCGCGTCCGGAGAGTGTTATATGGCGGAAACGACGGTTAAAGAATT
TGCGCAGACGGTAGGGATCC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSKEILLVVEATSNEKGVDREVIFEAIEAALASATRKRHLEDIDARVAVDRQSGDYATYRRWEVVPDEESVEEPQRQISL
ERARERDENAEVGGYVEEPIESVDFGRIAAQTAKQVIVQKVREAERAQIVDAYQHRIGELVNGSVKRMERGSAIIDLGEN
TEALVSREDLIPREAVRPNDRIRGYLRDVRSEPRGPQLFVSRTANEFLVELFKIEVPEVGQGLIEILGAARDPGMRAKIS
VRALDPRIDPVGACVGMRGSRVQAVSNELSGERIDIIPWDENPAQFVINALAPAEVESIVVDEDRGAMDVAVAEEQLSQA
IGRGGQNVRLASELTGWELNVMTAEEAEAKSEREAGELVQLFVEHLDVDEDVAGVLVQEGFSSIEEVAYVPTAELLEIEE
FDEDIVEALRSRARDVLLAQAIAEEANENQPSEELLALEGMDEQTAKALAERGVATVEDLADQSVDDLMEVEGMDEDRAG
QLIMKAREPWFAASEGGERAD

Sequences:

>Translated_501_residues
MSKEILLVVEATSNEKGVDREVIFEAIEAALASATRKRHLEDIDARVAVDRQSGDYATYRRWEVVPDEESVEEPQRQISL
ERARERDENAEVGGYVEEPIESVDFGRIAAQTAKQVIVQKVREAERAQIVDAYQHRIGELVNGSVKRMERGSAIIDLGEN
TEALVSREDLIPREAVRPNDRIRGYLRDVRSEPRGPQLFVSRTANEFLVELFKIEVPEVGQGLIEILGAARDPGMRAKIS
VRALDPRIDPVGACVGMRGSRVQAVSNELSGERIDIIPWDENPAQFVINALAPAEVESIVVDEDRGAMDVAVAEEQLSQA
IGRGGQNVRLASELTGWELNVMTAEEAEAKSEREAGELVQLFVEHLDVDEDVAGVLVQEGFSSIEEVAYVPTAELLEIEE
FDEDIVEALRSRARDVLLAQAIAEEANENQPSEELLALEGMDEQTAKALAERGVATVEDLADQSVDDLMEVEGMDEDRAG
QLIMKAREPWFAASEGGERAD
>Mature_500_residues
SKEILLVVEATSNEKGVDREVIFEAIEAALASATRKRHLEDIDARVAVDRQSGDYATYRRWEVVPDEESVEEPQRQISLE
RARERDENAEVGGYVEEPIESVDFGRIAAQTAKQVIVQKVREAERAQIVDAYQHRIGELVNGSVKRMERGSAIIDLGENT
EALVSREDLIPREAVRPNDRIRGYLRDVRSEPRGPQLFVSRTANEFLVELFKIEVPEVGQGLIEILGAARDPGMRAKISV
RALDPRIDPVGACVGMRGSRVQAVSNELSGERIDIIPWDENPAQFVINALAPAEVESIVVDEDRGAMDVAVAEEQLSQAI
GRGGQNVRLASELTGWELNVMTAEEAEAKSEREAGELVQLFVEHLDVDEDVAGVLVQEGFSSIEEVAYVPTAELLEIEEF
DEDIVEALRSRARDVLLAQAIAEEANENQPSEELLALEGMDEQTAKALAERGVATVEDLADQSVDDLMEVEGMDEDRAGQ
LIMKAREPWFAASEGGERAD

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=59.7165991902834, Blast_Score=583, Evalue=1e-168,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55361; Mature: 55230

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEILLVVEATSNEKGVDREVIFEAIEAALASATRKRHLEDIDARVAVDRQSGDYATYR
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEE
RWEVVPDEESVEEPQRQISLERARERDENAEVGGYVEEPIESVDFGRIAAQTAKQVIVQK
EEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
VREAERAQIVDAYQHRIGELVNGSVKRMERGSAIIDLGENTEALVSREDLIPREAVRPND
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEECCCCHHHHHHHHHCCCHHHCCCCH
RIRGYLRDVRSEPRGPQLFVSRTANEFLVELFKIEVPEVGQGLIEILGAARDPGMRAKIS
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEE
VRALDPRIDPVGACVGMRGSRVQAVSNELSGERIDIIPWDENPAQFVINALAPAEVESIV
EEEECCCCCCHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHEE
VDEDRGAMDVAVAEEQLSQAIGRGGQNVRLASELTGWELNVMTAEEAEAKSEREAGELVQ
EECCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
LFVEHLDVDEDVAGVLVQEGFSSIEEVAYVPTAELLEIEEFDEDIVEALRSRARDVLLAQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAEEANENQPSEELLALEGMDEQTAKALAERGVATVEDLADQSVDDLMEVEGMDEDRAG
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCHHH
QLIMKAREPWFAASEGGERAD
HHHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure 
SKEILLVVEATSNEKGVDREVIFEAIEAALASATRKRHLEDIDARVAVDRQSGDYATYR
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEE
RWEVVPDEESVEEPQRQISLERARERDENAEVGGYVEEPIESVDFGRIAAQTAKQVIVQK
EEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
VREAERAQIVDAYQHRIGELVNGSVKRMERGSAIIDLGENTEALVSREDLIPREAVRPND
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEECCCCHHHHHHHHHCCCHHHCCCCH
RIRGYLRDVRSEPRGPQLFVSRTANEFLVELFKIEVPEVGQGLIEILGAARDPGMRAKIS
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEE
VRALDPRIDPVGACVGMRGSRVQAVSNELSGERIDIIPWDENPAQFVINALAPAEVESIV
EEEECCCCCCHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHEE
VDEDRGAMDVAVAEEQLSQAIGRGGQNVRLASELTGWELNVMTAEEAEAKSEREAGELVQ
EECCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
LFVEHLDVDEDVAGVLVQEGFSSIEEVAYVPTAELLEIEEFDEDIVEALRSRARDVLLAQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAEEANENQPSEELLALEGMDEQTAKALAERGVATVEDLADQSVDDLMEVEGMDEDRAG
HHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCHHH
QLIMKAREPWFAASEGGERAD
HHHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]