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The map label for this gene is metF [H]

Identifier: 121998453

GI number: 121998453

Start: 1826335

End: 1827249

Strand: Reverse

Name: metF [H]

Synonym: Hhal_1674

Alternate gene names: 121998453

Gene position: 1827249-1826335 (Counterclockwise)

Preceding gene: 121998454

Following gene: 121998451

Centisome position: 68.22

GC content: 64.81

Gene sequence:

>915_bases
ATGGAAAGTCAAAAGAAACACCCCCGCGTCTTCAGTTTCGAGTTCTTCCCGCCCAAGACCGACGAGGGCGCGGAGAACCT
GAAGGGCACGCGGGAGCGCCTGGAGCGGCTGAACCCGGCTTACTTTTCGGTGACCTTCGGCGCCGGGGGGTCGACCCAGG
ATCGCACCTTCGAGACGGTGATCGACATCCAGCGCAACAGCACCGCCGACGCTGCGCCGCACCTGTCGTGCATCGACGCC
AGTCGGGATCGGCTCAAGCAGATGATCCTCGACTACAAAGAGCAGGGTGTGAAGTACATCGTCGCCCTGCGCGGCGATCG
GCCGTCCGGCTCCGGGTCCATGGGGCAGGGCGAGCTGAGTTACGCCAACGAGCTCGTCGAGCTGATCCGCGAGGTCACCG
GTGACCACTTCTACATCGAGGTGGGCGCCTATCCGGAGTTCCACCCGGAGGCCCCGGACGCACTGACCGATATCGAGAAC
TTCGTCCGCAAGGCCAAGGCCGGCGCGGACTCGGCCATCACGCAGTTCTTCTACAACGCGGACTGCTACTACCGCTTTGT
CGAGGATTGCGAGAAGCGCGGGGTGGACATCCCGATCGTCCCCGGCATCATGCCGCTGATGAACTTCGAGCAGCAGGCGC
GATTCGCCGCGGCCTGCAAGGCCGACATGCCGCGCTGGCTGGCACAGCGGATGGAGGCCTGGAAAGATGACCCACAGTCG
CGGATGGAGTACGGCATCGACGTCGTGACGCGGCTTTGCGAGGACCTGCTGGACAATGGTGCGCCCGGCATCCACTTCTA
CGCCTTGAACAAGGCGCCGGTGGTCGAGCGCATCTGGCACAACCTTGGGCTGTCGGAGCAGACCGGCGGCGAGGTGCGCA
AGCCGGACGAGGTCGAGGTGCCGGCGCAGCCGTAA

Upstream 100 bases:

>100_bases
CGGTTGAGCGCCCAGAGCGTTCAGCGTTCAGCGTTCGGGCCGGGCGGTCCGAACGTTGAACACTGAGCACTGAACGCTTC
GAGTCAATCGGAGGGGTTCA

Downstream 100 bases:

>100_bases
GGTCGGCGTCGGCGCCACAGATGCGCGCGTTCCGCGTTTGCCATCAAGACAACCCCCGGTGGGGCGTGGCCCCACCGGGG
GTTTTTTGATGAGCACTTCT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MESQKKHPRVFSFEFFPPKTDEGAENLKGTRERLERLNPAYFSVTFGAGGSTQDRTFETVIDIQRNSTADAAPHLSCIDA
SRDRLKQMILDYKEQGVKYIVALRGDRPSGSGSMGQGELSYANELVELIREVTGDHFYIEVGAYPEFHPEAPDALTDIEN
FVRKAKAGADSAITQFFYNADCYYRFVEDCEKRGVDIPIVPGIMPLMNFEQQARFAAACKADMPRWLAQRMEAWKDDPQS
RMEYGIDVVTRLCEDLLDNGAPGIHFYALNKAPVVERIWHNLGLSEQTGGEVRKPDEVEVPAQP

Sequences:

>Translated_304_residues
MESQKKHPRVFSFEFFPPKTDEGAENLKGTRERLERLNPAYFSVTFGAGGSTQDRTFETVIDIQRNSTADAAPHLSCIDA
SRDRLKQMILDYKEQGVKYIVALRGDRPSGSGSMGQGELSYANELVELIREVTGDHFYIEVGAYPEFHPEAPDALTDIEN
FVRKAKAGADSAITQFFYNADCYYRFVEDCEKRGVDIPIVPGIMPLMNFEQQARFAAACKADMPRWLAQRMEAWKDDPQS
RMEYGIDVVTRLCEDLLDNGAPGIHFYALNKAPVVERIWHNLGLSEQTGGEVRKPDEVEVPAQP
>Mature_304_residues
MESQKKHPRVFSFEFFPPKTDEGAENLKGTRERLERLNPAYFSVTFGAGGSTQDRTFETVIDIQRNSTADAAPHLSCIDA
SRDRLKQMILDYKEQGVKYIVALRGDRPSGSGSMGQGELSYANELVELIREVTGDHFYIEVGAYPEFHPEAPDALTDIEN
FVRKAKAGADSAITQFFYNADCYYRFVEDCEKRGVDIPIVPGIMPLMNFEQQARFAAACKADMPRWLAQRMEAWKDDPQS
RMEYGIDVVTRLCEDLLDNGAPGIHFYALNKAPVVERIWHNLGLSEQTGGEVRKPDEVEVPAQP

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=301, Percent_Identity=33.5548172757475, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1790377, Length=273, Percent_Identity=42.8571428571429, Blast_Score=233, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI25147436, Length=293, Percent_Identity=31.0580204778157, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25147441, Length=293, Percent_Identity=31.0580204778157, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321313, Length=280, Percent_Identity=31.7857142857143, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=31.3380281690141, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24662658, Length=225, Percent_Identity=27.1111111111111, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34268; Mature: 34268

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQKKHPRVFSFEFFPPKTDEGAENLKGTRERLERLNPAYFSVTFGAGGSTQDRTFETV
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHH
IDIQRNSTADAAPHLSCIDASRDRLKQMILDYKEQGVKYIVALRGDRPSGSGSMGQGELS
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHH
YANELVELIREVTGDHFYIEVGAYPEFHPEAPDALTDIENFVRKAKAGADSAITQFFYNA
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DCYYRFVEDCEKRGVDIPIVPGIMPLMNFEQQARFAAACKADMPRWLAQRMEAWKDDPQS
HHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHH
RMEYGIDVVTRLCEDLLDNGAPGIHFYALNKAPVVERIWHNLGLSEQTGGEVRKPDEVEV
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAQP
CCCC
>Mature Secondary Structure
MESQKKHPRVFSFEFFPPKTDEGAENLKGTRERLERLNPAYFSVTFGAGGSTQDRTFETV
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHH
IDIQRNSTADAAPHLSCIDASRDRLKQMILDYKEQGVKYIVALRGDRPSGSGSMGQGELS
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHH
YANELVELIREVTGDHFYIEVGAYPEFHPEAPDALTDIENFVRKAKAGADSAITQFFYNA
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DCYYRFVEDCEKRGVDIPIVPGIMPLMNFEQQARFAAACKADMPRWLAQRMEAWKDDPQS
HHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHH
RMEYGIDVVTRLCEDLLDNGAPGIHFYALNKAPVVERIWHNLGLSEQTGGEVRKPDEVEV
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PAQP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]