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The map label for this gene is ogt [H]

Identifier: 121998372

GI number: 121998372

Start: 1732191

End: 1732670

Strand: Reverse

Name: ogt [H]

Synonym: Hhal_1593

Alternate gene names: 121998372

Gene position: 1732670-1732191 (Counterclockwise)

Preceding gene: 121998373

Following gene: 121998371

Centisome position: 64.69

GC content: 73.12

Gene sequence:

>480_bases
GTGGGCGGCGATTCGGCGCCGCACGGGGTAGCGCGGCTGGCCGGTGTCTTCCTGGGGGTGTCCCTGGACGGCGAGGGGTG
TGTCGCCCGTATCCGGATCTCGTTACGGCTGCCTACGGCCGCGTCCGGGCCGGCCGACCGGCAGGTGCACGACGTGGTGC
GCTGTCTGGAGGCCTGGGAGCGGGATCCGTTGGCTCCGGCGCGGCTCCCCCGGGCACCGGCTGCTTCCCCGTTTCAGGCC
CGCCTGAGAGAGGCGCTGCTGGCCTTGGAGCCGGGGCAGACGGTTTCCTATGGGGAGCTGGCTCGGCATCTGGGCACATC
CCCTCGGGCGGTCGGCGCCGGGTGTCGCGCCAATCCGCTGCCCCTGCTGGTCCCGTGCCATCGAGTGGTCGGGAGCCGGG
GCGCCGGCGGCTATGCCGGTACGGCCGGAGGCAGTCTGACCCGGTTCAAGGCGTGGCTGCTGGCGCAGGAACAGCGATGA

Upstream 100 bases:

>100_bases
GATCACATCCCGAGCCGGGATATGGCCCTGCGCCCGCGTACCAAGCTCGAGGACGGCGAGTCGCCGGATCTGTTCTGAGC
AGCCGTCCGGGGTCGGGTCT

Downstream 100 bases:

>100_bases
TCGCCCTCCCGCGGACCCTCCCCTTGAAAAGCCGGTCAGGCGTTCCCACATCCGGTCGACACAACCCGCCGCCGGGATCC
GGCGAGCGAGCCCACGTGTT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MGGDSAPHGVARLAGVFLGVSLDGEGCVARIRISLRLPTAASGPADRQVHDVVRCLEAWERDPLAPARLPRAPAASPFQA
RLREALLALEPGQTVSYGELARHLGTSPRAVGAGCRANPLPLLVPCHRVVGSRGAGGYAGTAGGSLTRFKAWLLAQEQR

Sequences:

>Translated_159_residues
MGGDSAPHGVARLAGVFLGVSLDGEGCVARIRISLRLPTAASGPADRQVHDVVRCLEAWERDPLAPARLPRAPAASPFQA
RLREALLALEPGQTVSYGELARHLGTSPRAVGAGCRANPLPLLVPCHRVVGSRGAGGYAGTAGGSLTRFKAWLLAQEQR
>Mature_158_residues
GGDSAPHGVARLAGVFLGVSLDGEGCVARIRISLRLPTAASGPADRQVHDVVRCLEAWERDPLAPARLPRAPAASPFQAR
LREALLALEPGQTVSYGELARHLGTSPRAVGAGCRANPLPLLVPCHRVVGSRGAGGYAGTAGGSLTRFKAWLLAQEQR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=91, Percent_Identity=42.8571428571429, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788542, Length=123, Percent_Identity=38.2113821138211, Blast_Score=63, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=117, Percent_Identity=35.042735042735, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16573; Mature: 16442

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGDSAPHGVARLAGVFLGVSLDGEGCVARIRISLRLPTAASGPADRQVHDVVRCLEAWE
CCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
RDPLAPARLPRAPAASPFQARLREALLALEPGQTVSYGELARHLGTSPRAVGAGCRANPL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PLLVPCHRVVGSRGAGGYAGTAGGSLTRFKAWLLAQEQR
CEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
GGDSAPHGVARLAGVFLGVSLDGEGCVARIRISLRLPTAASGPADRQVHDVVRCLEAWE
CCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
RDPLAPARLPRAPAASPFQARLREALLALEPGQTVSYGELARHLGTSPRAVGAGCRANPL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PLLVPCHRVVGSRGAGGYAGTAGGSLTRFKAWLLAQEQR
CEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]