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The map label for this gene is ddlB [C]

Identifier: 121998283

GI number: 121998283

Start: 1623076

End: 1624122

Strand: Reverse

Name: ddlB [C]

Synonym: Hhal_1501

Alternate gene names: 121998283

Gene position: 1624122-1623076 (Counterclockwise)

Preceding gene: 121998290

Following gene: 121998280

Centisome position: 60.64

GC content: 68.39

Gene sequence:

>1047_bases
GTGAATGTACCCGCCCCCCTGCGCCTCGGTGTTCTCTTTGGCGACCCGCGTCTGCCCTACGCCTACGGTCCCTACGGGCG
GATCGGCCCCCAGGAGCTGGAGGCAGCCGCGGAGGCCCGGCGGGTGCTGGCGGCGCTGCCCGGGGTGACCCTGCGCGTAT
TCGACGACCACGAGCGGCTGATCGACGATCTGCGCGCCGACCCCCCAGAGCTGGCCGTAAATCTGTGCGATAACGGCTAC
TGCAATCGACTGGAACTCGAGGCGAATATCCCGGCGTTGCTGGAGCTGTTCGAGATCCCCTACACCGGTACCGGGGGGGC
TGCTATGGGCCTGAGCCGGGACAAGGCGGCGGTCCGTGCCCTGGCCGCCTCGCTGGGGGTAGCGGTGCCGGAGGAGCGCA
CCATCGACCTCAGCGACCCAACCCCGGCTCTACCCGAGCGTTATCCGGCGGTGATCAAGCCGAACGACGGCTGCGGCAGC
ATCGGCATGGAGCCGGATTGCGTGGTCGAGGACGCCCACCAGGCGCAGGCCTACCTGCGTCGGCTGGCAGGCTCGGGGGC
CGCCTGGGTGCTTGCTCAAGAGTACTTGCCTGGCGCCGAGTACACGGTGGGGTTGGTCGGCAATCCGGCTACCGGGTTGC
TGGTGTTACCGGCCCTGGTGGTCGACTACACAGCGCTGCCGCCGGAGCTGCCGCCGATCCTCTCCTACGGCTCCAAGGTC
GATCCCGCCTCCCCGTATTACCAGGCCTTGCGCTTTGCCCCGGCGCAACTGAGCGAAGAGAAACGCTCCGCTATGGAGGT
CGCGGCCCGGCGCCTGTTTGCCCGGGTTGGAGCCCGGGACTACGCCCGGGTGGACTTCCGCGAGGACCTGCGGGGCGTGC
CCCGCCTGCTCGATTTCAACAACCACCCCTCCTGGGGACCGGACGGCAAGTTGGCGATGATGGCTGGGTATGCAGGCTAC
TCCTACGCCGACTTGCTGGAACGGATTGTCCGGGCTGCCCTGGACCGCTATGCGGCCAGTGGCCGGGTAATCGGCCGTCG
CGACTAA

Upstream 100 bases:

>100_bases
CCGTTAAGAGCATAACACTTGCTGTGGACCGTGCGAGCGCCTGCTAGGTTTAGCAAAGGGCGCGCCGCGCGCCTTTCTGG
CTGTTTGGAGCGTGCCCACT

Downstream 100 bases:

>100_bases
AGCAGCTGCCGCGGGATCCATTCATCGTAGTGGCGGTGGAAGATGCGTTCCCCATTCTCGAACACCTCCAGCTCAGCGCT
GACCCGAAAGTGCTCGACAG

Product: hypothetical protein

Products: ADP; D-Alanyl-D-alanine; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNVPAPLRLGVLFGDPRLPYAYGPYGRIGPQELEAAAEARRVLAALPGVTLRVFDDHERLIDDLRADPPELAVNLCDNGY
CNRLELEANIPALLELFEIPYTGTGGAAMGLSRDKAAVRALAASLGVAVPEERTIDLSDPTPALPERYPAVIKPNDGCGS
IGMEPDCVVEDAHQAQAYLRRLAGSGAAWVLAQEYLPGAEYTVGLVGNPATGLLVLPALVVDYTALPPELPPILSYGSKV
DPASPYYQALRFAPAQLSEEKRSAMEVAARRLFARVGARDYARVDFREDLRGVPRLLDFNNHPSWGPDGKLAMMAGYAGY
SYADLLERIVRAALDRYAASGRVIGRRD

Sequences:

>Translated_348_residues
MNVPAPLRLGVLFGDPRLPYAYGPYGRIGPQELEAAAEARRVLAALPGVTLRVFDDHERLIDDLRADPPELAVNLCDNGY
CNRLELEANIPALLELFEIPYTGTGGAAMGLSRDKAAVRALAASLGVAVPEERTIDLSDPTPALPERYPAVIKPNDGCGS
IGMEPDCVVEDAHQAQAYLRRLAGSGAAWVLAQEYLPGAEYTVGLVGNPATGLLVLPALVVDYTALPPELPPILSYGSKV
DPASPYYQALRFAPAQLSEEKRSAMEVAARRLFARVGARDYARVDFREDLRGVPRLLDFNNHPSWGPDGKLAMMAGYAGY
SYADLLERIVRAALDRYAASGRVIGRRD
>Mature_348_residues
MNVPAPLRLGVLFGDPRLPYAYGPYGRIGPQELEAAAEARRVLAALPGVTLRVFDDHERLIDDLRADPPELAVNLCDNGY
CNRLELEANIPALLELFEIPYTGTGGAAMGLSRDKAAVRALAASLGVAVPEERTIDLSDPTPALPERYPAVIKPNDGCGS
IGMEPDCVVEDAHQAQAYLRRLAGSGAAWVLAQEYLPGAEYTVGLVGNPATGLLVLPALVVDYTALPPELPPILSYGSKV
DPASPYYQALRFAPAQLSEEKRSAMEVAARRLFARVGARDYARVDFREDLRGVPRLLDFNNHPSWGPDGKLAMMAGYAGY
SYADLLERIVRAALDRYAASGRVIGRRD

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=265, Percent_Identity=24.1509433962264, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4 [C]

Molecular weight: Translated: 37612; Mature: 37612

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPAPLRLGVLFGDPRLPYAYGPYGRIGPQELEAAAEARRVLAALPGVTLRVFDDHERL
CCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
IDDLRADPPELAVNLCDNGYCNRLELEANIPALLELFEIPYTGTGGAAMGLSRDKAAVRA
HHHHCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHH
LAASLGVAVPEERTIDLSDPTPALPERYPAVIKPNDGCGSIGMEPDCVVEDAHQAQAYLR
HHHHHCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
RLAGSGAAWVLAQEYLPGAEYTVGLVGNPATGLLVLPALVVDYTALPPELPPILSYGSKV
HHHCCCCEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
DPASPYYQALRFAPAQLSEEKRSAMEVAARRLFARVGARDYARVDFREDLRGVPRLLDFN
CCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCCHHHHCCC
NHPSWGPDGKLAMMAGYAGYSYADLLERIVRAALDRYAASGRVIGRRD
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNVPAPLRLGVLFGDPRLPYAYGPYGRIGPQELEAAAEARRVLAALPGVTLRVFDDHERL
CCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
IDDLRADPPELAVNLCDNGYCNRLELEANIPALLELFEIPYTGTGGAAMGLSRDKAAVRA
HHHHCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHH
LAASLGVAVPEERTIDLSDPTPALPERYPAVIKPNDGCGSIGMEPDCVVEDAHQAQAYLR
HHHHHCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
RLAGSGAAWVLAQEYLPGAEYTVGLVGNPATGLLVLPALVVDYTALPPELPPILSYGSKV
HHHCCCCEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
DPASPYYQALRFAPAQLSEEKRSAMEVAARRLFARVGARDYARVDFREDLRGVPRLLDFN
CCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCCHHHHCCC
NHPSWGPDGKLAMMAGYAGYSYADLLERIVRAALDRYAASGRVIGRRD
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1018 [C]

Specific activity: 31

Km value (mM): 6 {D-Phe}} 9 {D-Met}} 15 {D-norleucine}} 40 {ATP}} 2.5 {ATP}} 2.4 {ATP}} 0.179 {ATP}} 0.161 {ATP}} 0.055 {ATP}} 0.049 {ATP}} 0.033 {ATP}} 0.008 {ATP}} 86 {D-Ala}} 70.3 {D-Ala}} 60.4 {D-Ala}} 57.5 {D-

Substrates: D-Alanine; ATP [C]

Specific reaction: (2) D-Alanine + ATP <==> ADP + D-Alanyl-D-alanine + H+ + Phosphate [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: [1 (S)-Aminoethyl] [(2RS) 2-carboxy-1-octyl] phosphinic acid; Aminoalkyl phosphinate; D-Ala-D-2-hydroxy; D-Ala-D-Ala; D-Ala-D-Alaphosphinate; D-Cycloserine; Gly; Gly-Gly; Phosphinates; Phosphonate dipeptide analogs [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]