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The map label for this gene is grpE

Identifier: 121998259

GI number: 121998259

Start: 1598501

End: 1599223

Strand: Reverse

Name: grpE

Synonym: Hhal_1477

Alternate gene names: 121998259

Gene position: 1599223-1598501 (Counterclockwise)

Preceding gene: 121998260

Following gene: 121998258

Centisome position: 59.71

GC content: 68.46

Gene sequence:

>723_bases
ATGACTGATAACCAGCGACAGAGCACGACCGACGGGCAGCCCGAGACGCGCGATCCGGCGCAGGCGACGGCCGAGGCCGC
TGAACAGACCCAGGCCACCCAGGCCTCGGCGGCCGTCGAGGGCGAGCTCCAGGACGGGAGCGCGGATTCCGCCACGGGTG
CCAGCTTGTCGGCCGATGACGGCGCGGATCCCGAAGCCCTGCGACAACGCGTCGAGGAGCTCGAGAAGGCCCTGGCCGAT
GCCGAGCAGAAGGCCGAGGAGCACTGGGACCAGGTGCTGCGCATGCGAGCCGAGCTGGAGAACGCGCGGCGCCGCGCCGA
GAAAGACGTCGACCAGGCCAAGCGGCAGGGACTGGAGAAGGTCTGCGGGGATCTGCTGCAGGTCAAGGACAGCCTGGAGA
TGGGCGTGCAGGCGGCCGAAGACGCCGAGGCGGATCGCGAGAAGCTCCTCGAGGGCTCGCAGCTAACGCTCAAGATGCTC
AACCAGGTCTTTGAGCGCTTCGAGATCGAGGAGATCAACCCGCAGGGCGAGCGTTTCAATCCCGACTACCACGAGGCCAT
GGCGGCGCAGCCCAGCGACGAGCAGGAGCCGAATACCGTGCTCCAGGTGGTTCAGAAGGGCTACCGCCTGCAGGATCGCC
TGCTGCGCCCGGCGCTGGTGGTGGTCGCCAAGAAGGGCGACGGCGGCGCGGGCAGTGGCGGATCCGTGGATGAGACTGCC
TGA

Upstream 100 bases:

>100_bases
TGTCCAGGCAGGAGACAGCGCACCCGGCCGAGGCGCCCGAGCCGTCGGGCGGTGCGCTGCGTGTTGACCGTTTTCTGGCT
CAATCCAGCGGAGCAGCCAT

Downstream 100 bases:

>100_bases
GAGCCGGCGGATCGGTCGGTTTGAGTCCCGGCCCTTGAAAAAGCCGATCGGGCACCGCACTTAAGGGGCAGTAGGCAATC
AGGCCCGGACAGATTCAACG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTDNQRQSTTDGQPETRDPAQATAEAAEQTQATQASAAVEGELQDGSADSATGASLSADDGADPEALRQRVEELEKALAD
AEQKAEEHWDQVLRMRAELENARRRAEKDVDQAKRQGLEKVCGDLLQVKDSLEMGVQAAEDAEADREKLLEGSQLTLKML
NQVFERFEIEEINPQGERFNPDYHEAMAAQPSDEQEPNTVLQVVQKGYRLQDRLLRPALVVVAKKGDGGAGSGGSVDETA

Sequences:

>Translated_240_residues
MTDNQRQSTTDGQPETRDPAQATAEAAEQTQATQASAAVEGELQDGSADSATGASLSADDGADPEALRQRVEELEKALAD
AEQKAEEHWDQVLRMRAELENARRRAEKDVDQAKRQGLEKVCGDLLQVKDSLEMGVQAAEDAEADREKLLEGSQLTLKML
NQVFERFEIEEINPQGERFNPDYHEAMAAQPSDEQEPNTVLQVVQKGYRLQDRLLRPALVVVAKKGDGGAGSGGSVDETA
>Mature_239_residues
TDNQRQSTTDGQPETRDPAQATAEAAEQTQATQASAAVEGELQDGSADSATGASLSADDGADPEALRQRVEELEKALADA
EQKAEEHWDQVLRMRAELENARRRAEKDVDQAKRQGLEKVCGDLLQVKDSLEMGVQAAEDAEADREKLLEGSQLTLKMLN
QVFERFEIEEINPQGERFNPDYHEAMAAQPSDEQEPNTVLQVVQKGYRLQDRLLRPALVVVAKKGDGGAGSGGSVDETA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=196, Percent_Identity=34.6938775510204, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI40255109, Length=165, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788967, Length=156, Percent_Identity=39.7435897435897, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17552458, Length=159, Percent_Identity=33.9622641509434, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=158, Percent_Identity=32.2784810126582, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=39.873417721519, Blast_Score=110, Evalue=6e-25,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_HALHL (A1WX32)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003046.1
- ProteinModelPortal:   A1WX32
- SMR:   A1WX32
- STRING:   A1WX32
- GeneID:   4710632
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1477
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NDARLEQ
- PhylomeDB:   A1WX32
- ProtClustDB:   CLSK727505
- BioCyc:   HHAL349124:HHAL_1477-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 26160; Mature: 26029

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNQRQSTTDGQPETRDPAQATAEAAEQTQATQASAAVEGELQDGSADSATGASLSADD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GADPEALRQRVEELEKALADAEQKAEEHWDQVLRMRAELENARRRAEKDVDQAKRQGLEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCGDLLQVKDSLEMGVQAAEDAEADREKLLEGSQLTLKMLNQVFERFEIEEINPQGERFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCCCCCCCC
PDYHEAMAAQPSDEQEPNTVLQVVQKGYRLQDRLLRPALVVVAKKGDGGAGSGGSVDETA
CHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDNQRQSTTDGQPETRDPAQATAEAAEQTQATQASAAVEGELQDGSADSATGASLSADD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GADPEALRQRVEELEKALADAEQKAEEHWDQVLRMRAELENARRRAEKDVDQAKRQGLEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCGDLLQVKDSLEMGVQAAEDAEADREKLLEGSQLTLKMLNQVFERFEIEEINPQGERFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCCCCCCCC
PDYHEAMAAQPSDEQEPNTVLQVVQKGYRLQDRLLRPALVVVAKKGDGGAGSGGSVDETA
CHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA