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The map label for this gene is eno

Identifier: 121998219

GI number: 121998219

Start: 1548051

End: 1549337

Strand: Reverse

Name: eno

Synonym: Hhal_1437

Alternate gene names: 121998219

Gene position: 1549337-1548051 (Counterclockwise)

Preceding gene: 121998220

Following gene: 121998218

Centisome position: 57.84

GC content: 66.51

Gene sequence:

>1287_bases
ATGGCGAACATCGAGCGTGTCATCGGCCGCGAGGTCCTCGACTCTCGCGGTAACCCGACCGTCGAGGCCGACGTCTGGCT
GGACGACGGCAGCTTCGGTCGGGCCGCGGTGCCCTCCGGCGCCTCCACCGGGAGCCGTGAGGCGGTCGAGCTGCGTGACG
GCGGGGGGCGTTACAACGGCAAGGGGGTGCGCACCGCGGTGGAGAACGTCAACACCACCATCCGCGAGGCGCTCCAGGGG
GGGAGTGCCGACGATCAGGCCGCTCTGGACCGGCAGCTGATCGACCTCGACGGCAGCCCCAACAAGGACCGTCTTGGTGC
CAACGCCATCCTCGCCGTATCCATGGCCGTCGCCCAGGCGGCAGCGCGCAGTCGCGGTCTGCCCCTCTATCGCCACCTGC
AGCAGGAGGGGTCCGCTGCCCAGCTGCCGGCCCCGATGATGAACATCCTCAACGGTGGGGAGCACGCCGACAACAGCGTG
GATATCCAGGAGTTCATGATCATGCCCATCGGGTTTGATCGGTTCTCCGAGGCGCTGCGCTGCGGTGCCGAGATCTTCCA
TGCGCTCAAAAAGGTCCTCCACGAGCGTGGGCTCTCCACTGCCGTGGGCGATGAAGGTGGCTTCGCCCCGGATCTGCCCT
CCAACGAGGCGGCCCTGGAAGCCATCATCGAGGCCATTCAGCGCGCCGGCTATCAGGCCGGTGATCAGGTGGCCCTGGCT
CTGGACGTGGCCAGCTCGGAACTCTATCGCGACGGTCGCTATCACCTCGCCTCGGAAGGGCGGGACTTCGACGCCGCCGG
GTTCGCCGATTACCTGGCGCAGCTCGTCGATAAGTATCCGATCGTCTCCATCGAGGACGGCATGGATGAGTCGGACTGGG
ACGGCTGGAAGGTGCTGACCGAGCGCCTGGGTGGTCAGATTCAATTGGTGGGCGACGACCTGTTCGTCACCAACCCGCAG
ATTCTGCAGCAGGGGATCGACCGCGGCATCGCTAACTCCATCCTGATCAAGCTCAATCAGATCGGGACCGTGACCGAGAC
CCTGGAGGCGATCCGCATGGCCGACGAGGCCGGCTACACTTCGGTGGTTTCTCACCGCTCCGGGGAGACCGAGGATGTGG
CGATCGCCGACCTGGCCGTGGCCACGCGGGCGACACAGATCAAGACCGGCTCGTTGTGCCGCTCCGATCGGGTGGCCAAG
TACAACCAGCTGCTGCGGATCGAGCAGGAGCTCGGCGACGACGCCCGGTACGTGGGTCGAGACGCGCTGCGTCCTGAGCA
GGGGTAG

Upstream 100 bases:

>100_bases
GCCGCTGGATCGTATGCGCGAACTCCTCGCGACGCTGATCGCGCTGGATCGCGTCGTCAAGGAGCAGGGATTCGCCGAGC
AATGAAGGAGCGACTGAATC

Downstream 100 bases:

>100_bases
GTCAGTGATCGCCGCGTGGGGCCGTCCCAGGAGGGCGCCATGCGTTGGGTGAACGGGTTGCTCGCTGTGCTGCTGGTCCT
CTTGCAGGCCCAGCTCTGGT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MANIERVIGREVLDSRGNPTVEADVWLDDGSFGRAAVPSGASTGSREAVELRDGGGRYNGKGVRTAVENVNTTIREALQG
GSADDQAALDRQLIDLDGSPNKDRLGANAILAVSMAVAQAAARSRGLPLYRHLQQEGSAAQLPAPMMNILNGGEHADNSV
DIQEFMIMPIGFDRFSEALRCGAEIFHALKKVLHERGLSTAVGDEGGFAPDLPSNEAALEAIIEAIQRAGYQAGDQVALA
LDVASSELYRDGRYHLASEGRDFDAAGFADYLAQLVDKYPIVSIEDGMDESDWDGWKVLTERLGGQIQLVGDDLFVTNPQ
ILQQGIDRGIANSILIKLNQIGTVTETLEAIRMADEAGYTSVVSHRSGETEDVAIADLAVATRATQIKTGSLCRSDRVAK
YNQLLRIEQELGDDARYVGRDALRPEQG

Sequences:

>Translated_428_residues
MANIERVIGREVLDSRGNPTVEADVWLDDGSFGRAAVPSGASTGSREAVELRDGGGRYNGKGVRTAVENVNTTIREALQG
GSADDQAALDRQLIDLDGSPNKDRLGANAILAVSMAVAQAAARSRGLPLYRHLQQEGSAAQLPAPMMNILNGGEHADNSV
DIQEFMIMPIGFDRFSEALRCGAEIFHALKKVLHERGLSTAVGDEGGFAPDLPSNEAALEAIIEAIQRAGYQAGDQVALA
LDVASSELYRDGRYHLASEGRDFDAAGFADYLAQLVDKYPIVSIEDGMDESDWDGWKVLTERLGGQIQLVGDDLFVTNPQ
ILQQGIDRGIANSILIKLNQIGTVTETLEAIRMADEAGYTSVVSHRSGETEDVAIADLAVATRATQIKTGSLCRSDRVAK
YNQLLRIEQELGDDARYVGRDALRPEQG
>Mature_427_residues
ANIERVIGREVLDSRGNPTVEADVWLDDGSFGRAAVPSGASTGSREAVELRDGGGRYNGKGVRTAVENVNTTIREALQGG
SADDQAALDRQLIDLDGSPNKDRLGANAILAVSMAVAQAAARSRGLPLYRHLQQEGSAAQLPAPMMNILNGGEHADNSVD
IQEFMIMPIGFDRFSEALRCGAEIFHALKKVLHERGLSTAVGDEGGFAPDLPSNEAALEAIIEAIQRAGYQAGDQVALAL
DVASSELYRDGRYHLASEGRDFDAAGFADYLAQLVDKYPIVSIEDGMDESDWDGWKVLTERLGGQIQLVGDDLFVTNPQI
LQQGIDRGIANSILIKLNQIGTVTETLEAIRMADEAGYTSVVSHRSGETEDVAIADLAVATRATQIKTGSLCRSDRVAKY
NQLLRIEQELGDDARYVGRDALRPEQG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=49.8850574712644, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=49.1954022988506, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=49.1954022988506, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=48.5057471264368, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=44.8036951501155, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.7857142857143, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.7857142857143, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.7857142857143, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=61.3053613053613, Blast_Score=521, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=48.1651376146789, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17536383, Length=436, Percent_Identity=48.1651376146789, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32563855, Length=236, Percent_Identity=43.2203389830509, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=47.5750577367206, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=47.5750577367206, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=47.3441108545035, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=47.3441108545035, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=47.3441108545035, Blast_Score=348, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=47.7064220183486, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=47.7064220183486, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=47.7064220183486, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=47.7064220183486, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281360527, Length=436, Percent_Identity=47.7064220183486, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17137654, Length=436, Percent_Identity=47.7064220183486, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_HALHL (A1WWZ2)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003006.1
- ProteinModelPortal:   A1WWZ2
- STRING:   A1WWZ2
- GeneID:   4709709
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1437
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   A1WWZ2
- ProtClustDB:   PRK00077
- BioCyc:   HHAL349124:HHAL_1437-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45979; Mature: 45848

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIERVIGREVLDSRGNPTVEADVWLDDGSFGRAAVPSGASTGSREAVELRDGGGRYNG
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
KGVRTAVENVNTTIREALQGGSADDQAALDRQLIDLDGSPNKDRLGANAILAVSMAVAQA
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHH
AARSRGLPLYRHLQQEGSAAQLPAPMMNILNGGEHADNSVDIQEFMIMPIGFDRFSEALR
HHHHCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHH
CGAEIFHALKKVLHERGLSTAVGDEGGFAPDLPSNEAALEAIIEAIQRAGYQAGDQVALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSELYRDGRYHLASEGRDFDAAGFADYLAQLVDKYPIVSIEDGMDESDWDGWKVLT
EEHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
ERLGGQIQLVGDDLFVTNPQILQQGIDRGIANSILIKLNQIGTVTETLEAIRMADEAGYT
HHHCCEEEEEECCEEECCHHHHHHHHHHHHHHHHHEEHHHCCCHHHHHHHHHHHHHCCHH
SVVSHRSGETEDVAIADLAVATRATQIKTGSLCRSDRVAKYNQLLRIEQELGDDARYVGR
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCH
DALRPEQG
HCCCCCCC
>Mature Secondary Structure 
ANIERVIGREVLDSRGNPTVEADVWLDDGSFGRAAVPSGASTGSREAVELRDGGGRYNG
CCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
KGVRTAVENVNTTIREALQGGSADDQAALDRQLIDLDGSPNKDRLGANAILAVSMAVAQA
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHH
AARSRGLPLYRHLQQEGSAAQLPAPMMNILNGGEHADNSVDIQEFMIMPIGFDRFSEALR
HHHHCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHEECCCCHHHHHHHHH
CGAEIFHALKKVLHERGLSTAVGDEGGFAPDLPSNEAALEAIIEAIQRAGYQAGDQVALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVASSELYRDGRYHLASEGRDFDAAGFADYLAQLVDKYPIVSIEDGMDESDWDGWKVLT
EEHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
ERLGGQIQLVGDDLFVTNPQILQQGIDRGIANSILIKLNQIGTVTETLEAIRMADEAGYT
HHHCCEEEEEECCEEECCHHHHHHHHHHHHHHHHHEEHHHCCCHHHHHHHHHHHHHCCHH
SVVSHRSGETEDVAIADLAVATRATQIKTGSLCRSDRVAKYNQLLRIEQELGDDARYVGR
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCH
DALRPEQG
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA