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The map label for this gene is pcm

Identifier: 121998212

GI number: 121998212

Start: 1543414

End: 1544076

Strand: Reverse

Name: pcm

Synonym: Hhal_1430

Alternate gene names: 121998212

Gene position: 1544076-1543414 (Counterclockwise)

Preceding gene: 121998213

Following gene: 121998211

Centisome position: 57.65

GC content: 69.53

Gene sequence:

>663_bases
ATGCGCGAGCGGAACGCAGCGGGGATTGGCATGACCTCTCAACGGACCCGGGATCGGCTGGTGGATGCATTGGCGGCCCA
GGGTATCCAGGACGAACGGGTGCTCTCGGCGATGCGCGAGGTGCCCCGGCACCTGTTCGTCGACGAAGCCCTGGAGAGCC
GCGCCTACGAGAATACCCCGCTACCCATTGGCGAGGGGCAGACGATCTCCCAGCCCTGGGTGGTCGCACGGATGACCGAG
CTGCTGCTCGAGCCGGGGGTCCCGGAGCGGGTGTTGGAGGTGGGGACCGGCTCCGGCTACCAGGCCGCGGTGTTGGCCCG
GCTGGTTCCGCGGGTCTACAGCATCGAGCGGATCGGTTCCCTGCTGCGCCGGGCCCGAGAGCGGCTGCAGGCGGTCCGCC
TGTTCAACTGCCAGCTTCGCCACGGTGATGGCTATGAGGGGTGGCCGGAGTACGCCCCCTACGACGGCATCATCGTGACC
GCGGCGCCGGACGCGCTGCCGGAGGCGCTGCTTGAGCAGCTCGCCGACGGGGGGCGTTTGGTTGCACCGATCGGTGGCGC
CGGCTATCAGGAGCTTCTGGTGGTGGATCGTCGGGGGGATGCGTACGAGCAGCGCCGGGTGGCCGGAGTCTCGTTCGTGC
CGATGCTTGAGGGGCGGGTCTGA

Upstream 100 bases:

>100_bases
TACGCAACGGGTTTGTCTCGGTGACCCCGATCCAGGTCGATCTAACCCGGCATGCGGGGCTCGAGCAGGTCCGCGGCTGG
CTGGACGGGGTGACGTGGTG

Downstream 100 bases:

>100_bases
GTGCGAGTGTTCGGGCCGTTGTACAGCTGGACGGTGCGGTGGGCCGGCCACCGTCGGGCCCCCTGGGCGCTGGGTGGGCT
CAGTGTCGCTGAGTCCTCGG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MRERNAAGIGMTSQRTRDRLVDALAAQGIQDERVLSAMREVPRHLFVDEALESRAYENTPLPIGEGQTISQPWVVARMTE
LLLEPGVPERVLEVGTGSGYQAAVLARLVPRVYSIERIGSLLRRARERLQAVRLFNCQLRHGDGYEGWPEYAPYDGIIVT
AAPDALPEALLEQLADGGRLVAPIGGAGYQELLVVDRRGDAYEQRRVAGVSFVPMLEGRV

Sequences:

>Translated_220_residues
MRERNAAGIGMTSQRTRDRLVDALAAQGIQDERVLSAMREVPRHLFVDEALESRAYENTPLPIGEGQTISQPWVVARMTE
LLLEPGVPERVLEVGTGSGYQAAVLARLVPRVYSIERIGSLLRRARERLQAVRLFNCQLRHGDGYEGWPEYAPYDGIIVT
AAPDALPEALLEQLADGGRLVAPIGGAGYQELLVVDRRGDAYEQRRVAGVSFVPMLEGRV
>Mature_220_residues
MRERNAAGIGMTSQRTRDRLVDALAAQGIQDERVLSAMREVPRHLFVDEALESRAYENTPLPIGEGQTISQPWVVARMTE
LLLEPGVPERVLEVGTGSGYQAAVLARLVPRVYSIERIGSLLRRARERLQAVRLFNCQLRHGDGYEGWPEYAPYDGIIVT
AAPDALPEALLEQLADGGRLVAPIGGAGYQELLVVDRRGDAYEQRRVAGVSFVPMLEGRV

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=216, Percent_Identity=32.4074074074074, Blast_Score=84, Evalue=7e-17,
Organism=Escherichia coli, GI1789100, Length=211, Percent_Identity=54.9763033175355, Blast_Score=207, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI71983477, Length=215, Percent_Identity=32.093023255814, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI193207222, Length=214, Percent_Identity=30.8411214953271, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17981723, Length=210, Percent_Identity=36.1904761904762, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_HALHL (A1WWY5)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002999.1
- ProteinModelPortal:   A1WWY5
- STRING:   A1WWY5
- GeneID:   4710549
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1430
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   YSVERIR
- PhylomeDB:   A1WWY5
- ProtClustDB:   CLSK727475
- BioCyc:   HHAL349124:HHAL_1430-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24274; Mature: 24274

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 70-70

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERNAAGIGMTSQRTRDRLVDALAAQGIQDERVLSAMREVPRHLFVDEALESRAYENTP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LPIGEGQTISQPWVVARMTELLLEPGVPERVLEVGTGSGYQAAVLARLVPRVYSIERIGS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LLRRARERLQAVRLFNCQLRHGDGYEGWPEYAPYDGIIVTAAPDALPEALLEQLADGGRL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEE
VAPIGGAGYQELLVVDRRGDAYEQRRVAGVSFVPMLEGRV
EEECCCCCCEEEEEECCCCCHHHHHHHCCCEEHHHCCCCC
>Mature Secondary Structure
MRERNAAGIGMTSQRTRDRLVDALAAQGIQDERVLSAMREVPRHLFVDEALESRAYENTP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LPIGEGQTISQPWVVARMTELLLEPGVPERVLEVGTGSGYQAAVLARLVPRVYSIERIGS
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LLRRARERLQAVRLFNCQLRHGDGYEGWPEYAPYDGIIVTAAPDALPEALLEQLADGGRL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEE
VAPIGGAGYQELLVVDRRGDAYEQRRVAGVSFVPMLEGRV
EEECCCCCCEEEEEECCCCCHHHHHHHCCCEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA