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The map label for this gene is cbiH [H]

Identifier: 121998126

GI number: 121998126

Start: 1454205

End: 1455512

Strand: Reverse

Name: cbiH [H]

Synonym: Hhal_1344

Alternate gene names: 121998126

Gene position: 1455512-1454205 (Counterclockwise)

Preceding gene: 121998127

Following gene: 121998125

Centisome position: 54.34

GC content: 70.8

Gene sequence:

>1308_bases
ATGACAGGCGATGTTCCCCCTCCGGGGCGGATCCTTCTGGTAGGGATCGGCCCGGGTGACCCGGCGCACATGACGGCGCG
GGCCCGCGAGGCGATCGCCGAGGCCGAGGTGGTCATCGGTTACCAGACGTACCTCAAACTGATCCCGGACCTGCTGGAAG
GCAAGACGGTGATCGGCAAGGCCATGACCGAAGAGCTCGATCGCGTGGAGGCGGCCTGGGAGGCTGCCCGAGGCGGGCAG
GTTGTGGCGCTGGTCTCCTCGGGCGACCCCGGGGTCTACGGCATGGCAGGGCCGACCTACGAGTTTCTGCTTCAGCACGG
CTGGCAGGGCCGCGCCGGGGAGGTGCAGGTGGAGGTGGTCCCCGGGGCCTCGGCGCTCTCCACCTGCGCCGCCCTGGTCG
GCGCGCCCCTGACCCACGACTTCTGCACCATCTCGCTCTCGGATCTGCTCACCTCCTGGCCAGTGATCGTGCGCCGGCTC
GAGGCCGCCGGGCGGGGGGATTTCGTTACGGTTCTCTACAACCCGGCGAGCAAGCGGCGGCAGGCGCACATCGGCCGCGC
GCAGGAGATCCTGTTGCGTTACCGCGACCCGCAAACCCCAGTGGCGGTGGTCCGGTCGGCCTATCGGGAGCGGCAGCAGG
TCCAGCATCTGTGCCTGGAGGAGCTCCATCGCGCCGAGCCGAGCATGCTCAGCACGGTGATCATCGGCAACCGTTCGACC
CGCTGCGAGCACGGGCTGATGGTGACCCCCCGGGGTTATACGGCCAAGTACGCCGAGGGCGGTGCCCCCGTGTCGGGGGA
GCAGCGCGGGCAATCGCTTAGCCTGGGGCTGGACGGATGGCCTGTGGTCCTGCGGCAATGGTTGCGCGAACAGCCGGCGC
CGAGCCTGGAGGCCGCTGCGGCGCATTTCGACGTCAACTGGGCGGAGGCGCTGGCGGCCGTGGCGGATGACGTAGCGGAG
CCGGATACCCCGTTTCTGACCAGTCGGATTGAGGTCTCGGACCTGGCGGCCCATCTTGAGGAGTGCCGCGAGTGGCCCCG
AATCCGGGCCGTGGTGCCGGGGCCGGCCGGAACGCGGATGGTCTGCTCGGTGGCGGGGGCCGGGCTCTACTACGACGGTG
AGACCCTGGTCCTCGGCGCCGGCGGAGGCCGGGTCGAACTCCACTGGCAGCGCGTGGCCGGGGCGTGGGCCCTGGCCGGC
GCCTGCCAGCACTGCAGCCTCGAGCTGGTCGATGCCCGCGGCCGGGGGCTCTTGTCCCTGCAGCGGGTGACGGGGAAGAA
CCGACCCATGGACCCATCCGGGGGGTGA

Upstream 100 bases:

>100_bases
GTCGGGCCCATGCCGACAGCGCCGATCTGATCATCGAGAAACGCCGCTGGCGGGGCGAAGACGGACGCAACGCCACGGTA
TCGATAGCGAGGCTGAACGA

Downstream 100 bases:

>100_bases
ACTGAACCCATGCACCACTACCCGATCTTCCTCAAGCTCCACGGTTGCCGCTGCCTGGTTGTGGGCGGCGGCGAGGCGGC
TGCGCGCAGGGCCGGGGATC

Product: precorrin-3B C17-methyltransferase

Products: 2S-adenosyl-L-homocysteine; precorrin; sirohydrochlorin; NADH; Proton; siroheme [C]

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTGDVPPPGRILLVGIGPGDPAHMTARAREAIAEAEVVIGYQTYLKLIPDLLEGKTVIGKAMTEELDRVEAAWEAARGGQ
VVALVSSGDPGVYGMAGPTYEFLLQHGWQGRAGEVQVEVVPGASALSTCAALVGAPLTHDFCTISLSDLLTSWPVIVRRL
EAAGRGDFVTVLYNPASKRRQAHIGRAQEILLRYRDPQTPVAVVRSAYRERQQVQHLCLEELHRAEPSMLSTVIIGNRST
RCEHGLMVTPRGYTAKYAEGGAPVSGEQRGQSLSLGLDGWPVVLRQWLREQPAPSLEAAAAHFDVNWAEALAAVADDVAE
PDTPFLTSRIEVSDLAAHLEECREWPRIRAVVPGPAGTRMVCSVAGAGLYYDGETLVLGAGGGRVELHWQRVAGAWALAG
ACQHCSLELVDARGRGLLSLQRVTGKNRPMDPSGG

Sequences:

>Translated_435_residues
MTGDVPPPGRILLVGIGPGDPAHMTARAREAIAEAEVVIGYQTYLKLIPDLLEGKTVIGKAMTEELDRVEAAWEAARGGQ
VVALVSSGDPGVYGMAGPTYEFLLQHGWQGRAGEVQVEVVPGASALSTCAALVGAPLTHDFCTISLSDLLTSWPVIVRRL
EAAGRGDFVTVLYNPASKRRQAHIGRAQEILLRYRDPQTPVAVVRSAYRERQQVQHLCLEELHRAEPSMLSTVIIGNRST
RCEHGLMVTPRGYTAKYAEGGAPVSGEQRGQSLSLGLDGWPVVLRQWLREQPAPSLEAAAAHFDVNWAEALAAVADDVAE
PDTPFLTSRIEVSDLAAHLEECREWPRIRAVVPGPAGTRMVCSVAGAGLYYDGETLVLGAGGGRVELHWQRVAGAWALAG
ACQHCSLELVDARGRGLLSLQRVTGKNRPMDPSGG
>Mature_434_residues
TGDVPPPGRILLVGIGPGDPAHMTARAREAIAEAEVVIGYQTYLKLIPDLLEGKTVIGKAMTEELDRVEAAWEAARGGQV
VALVSSGDPGVYGMAGPTYEFLLQHGWQGRAGEVQVEVVPGASALSTCAALVGAPLTHDFCTISLSDLLTSWPVIVRRLE
AAGRGDFVTVLYNPASKRRQAHIGRAQEILLRYRDPQTPVAVVRSAYRERQQVQHLCLEELHRAEPSMLSTVIIGNRSTR
CEHGLMVTPRGYTAKYAEGGAPVSGEQRGQSLSLGLDGWPVVLRQWLREQPAPSLEAAAAHFDVNWAEALAAVADDVAEP
DTPFLTSRIEVSDLAAHLEECREWPRIRAVVPGPAGTRMVCSVAGAGLYYDGETLVLGAGGGRVELHWQRVAGAWALAGA
CQHCSLELVDARGRGLLSLQRVTGKNRPMDPSGG

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=247, Percent_Identity=28.3400809716599, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.107; 1.3.1.76; 4.99.1.4 [C]

Molecular weight: Translated: 46603; Mature: 46471

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDVPPPGRILLVGIGPGDPAHMTARAREAIAEAEVVIGYQTYLKLIPDLLEGKTVIGK
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHCCCHHHHH
AMTEELDRVEAAWEAARGGQVVALVSSGDPGVYGMAGPTYEFLLQHGWQGRAGEVQVEVV
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCCHHHHHHHCCCCCCCCEEEEEEE
PGASALSTCAALVGAPLTHDFCTISLSDLLTSWPVIVRRLEAAGRGDFVTVLYNPASKRR
CCCHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
QAHIGRAQEILLRYRDPQTPVAVVRSAYRERQQVQHLCLEELHRAEPSMLSTVIIGNRST
HHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
RCEHGLMVTPRGYTAKYAEGGAPVSGEQRGQSLSLGLDGWPVVLRQWLREQPAPSLEAAA
CCCCCEEECCCCCEECCCCCCCCCCCHHCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHH
AHFDVNWAEALAAVADDVAEPDTPFLTSRIEVSDLAAHLEECREWPRIRAVVPGPAGTRM
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHH
VCSVAGAGLYYDGETLVLGAGGGRVELHWQRVAGAWALAGACQHCSLELVDARGRGLLSL
EEECCCCCEEECCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCEEE
QRVTGKNRPMDPSGG
HHHCCCCCCCCCCCC
>Mature Secondary Structure 
TGDVPPPGRILLVGIGPGDPAHMTARAREAIAEAEVVIGYQTYLKLIPDLLEGKTVIGK
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHCCCHHHHH
AMTEELDRVEAAWEAARGGQVVALVSSGDPGVYGMAGPTYEFLLQHGWQGRAGEVQVEVV
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECCCCCHHHHHHHCCCCCCCCEEEEEEE
PGASALSTCAALVGAPLTHDFCTISLSDLLTSWPVIVRRLEAAGRGDFVTVLYNPASKRR
CCCHHHHHHHHHHCCCCCCCHHEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
QAHIGRAQEILLRYRDPQTPVAVVRSAYRERQQVQHLCLEELHRAEPSMLSTVIIGNRST
HHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
RCEHGLMVTPRGYTAKYAEGGAPVSGEQRGQSLSLGLDGWPVVLRQWLREQPAPSLEAAA
CCCCCEEECCCCCEECCCCCCCCCCCHHCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHH
AHFDVNWAEALAAVADDVAEPDTPFLTSRIEVSDLAAHLEECREWPRIRAVVPGPAGTRM
HHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHH
VCSVAGAGLYYDGETLVLGAGGGRVELHWQRVAGAWALAGACQHCSLELVDARGRGLLSL
EEECCCCCEEECCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCEEE
QRVTGKNRPMDPSGG
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2S-Adenosyl-L-methionine; uroporphyrin III; dihydrosirohydrochlorin; NAD; sirohydrochlorin; Fe (II) [C]

Specific reaction: 2S-Adenosyl-L-methionine + uroporphyrin III = 2S-adenosyl-L-homocysteine + precorrin dihydrosirohydrochlorin + NAD = sirohydrochlorin + NADH sirohydrochlorin + Fe (II) = 2 Proton + siroheme [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]