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The map label for this gene is rsmA

Identifier: 121997814

GI number: 121997814

Start: 1096846

End: 1097643

Strand: Reverse

Name: rsmA

Synonym: Hhal_1023

Alternate gene names: 121997814

Gene position: 1097643-1096846 (Counterclockwise)

Preceding gene: 121997815

Following gene: 121997810

Centisome position: 40.98

GC content: 71.8

Gene sequence:

>798_bases
TTGAGCCATCGCCCACGCAAGCGCTTCGGGCAGAACTTCCTCCGCGACCCGGCGGTCATCCAGCGTATGGTCACGGCCAT
CGCCCCGCGGGAGGGGGAGACGCTGCTGGAGATCGGCCCCGGCGAGGGGGCGTTGACCGAACCGCTGCTGGCGCGCCTGG
GCACGCTCACCGCGGTGGAGCTGGATCGCGATCTGGCGCCCCGGTTGCAGGAGCGGTTTGCCCCGGCGCTGAGGGTGATC
GAGGGCGACGCCCTGGCACTGGATCCGGCCGAACTGGCTCCGGCCCAGGGCCGGCTGCGGGTGGTGGGCAACCTGCCCTA
CAACGTCTCGACGCCGATCCTCTTCCATCTGCTCGCTGCGGCCGATGTCATCGAGGATCTGCATCTGCTCCTCCAGCGCG
AGGTGGTGGATCGGATGGTGGCGCCGCCAGGGGGCAAGACCCGCGGGCGGCTGTCGGTGATGGTCCAATACCGGTGTCGG
GTGGAGCGCTGTTTCAATGTGCCCGCCGGGGCGTTCTTCCCGGCGCCGAAGGTGATGTCGTCGTTTGTCCGCCTGGTGCC
GCACCGGCCACTGCCGGCGCGGGCACAGGACGAGGCGTGGCTGCAGGAGGTGGTCACGGCGGCCTTCGGCGGGCGGCGCA
AGACGCTGCGCAACAGCCTCAAGGGGTTGGTGACGGCGCAGGCGTTCGAGGCGGCCGGGGTCGATCCCGGGGCGCGGGCG
GAGACGCTGTCGGTGGAGGCTTTCGTGCGTCTGGCCGATGCCGCACGGAGCGCGCCGGCGGCCGCCGCAGGGCGGTGA

Upstream 100 bases:

>100_bases
TGCTCACCCTCGACGGCTCGGTCGCGGCGCGCTCCCACGTTGGCGGCACGGCGCCGGAGCAGGTCCGTGCCCAGGCCCAG
GCGGCCCGGGAGCGCCTGGC

Downstream 100 bases:

>100_bases
GAAGGAAGGGCACGGACAGGCCCGCCTGCTCGACGGGGATGGGGACGCCGGGGGTTGGCCGGGGGCGGACGAGCGCGCCC
CGCCTACGGCGCGGTCCCCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSHRPRKRFGQNFLRDPAVIQRMVTAIAPREGETLLEIGPGEGALTEPLLARLGTLTAVELDRDLAPRLQERFAPALRVI
EGDALALDPAELAPAQGRLRVVGNLPYNVSTPILFHLLAAADVIEDLHLLLQREVVDRMVAPPGGKTRGRLSVMVQYRCR
VERCFNVPAGAFFPAPKVMSSFVRLVPHRPLPARAQDEAWLQEVVTAAFGGRRKTLRNSLKGLVTAQAFEAAGVDPGARA
ETLSVEAFVRLADAARSAPAAAAGR

Sequences:

>Translated_265_residues
MSHRPRKRFGQNFLRDPAVIQRMVTAIAPREGETLLEIGPGEGALTEPLLARLGTLTAVELDRDLAPRLQERFAPALRVI
EGDALALDPAELAPAQGRLRVVGNLPYNVSTPILFHLLAAADVIEDLHLLLQREVVDRMVAPPGGKTRGRLSVMVQYRCR
VERCFNVPAGAFFPAPKVMSSFVRLVPHRPLPARAQDEAWLQEVVTAAFGGRRKTLRNSLKGLVTAQAFEAAGVDPGARA
ETLSVEAFVRLADAARSAPAAAAGR
>Mature_264_residues
SHRPRKRFGQNFLRDPAVIQRMVTAIAPREGETLLEIGPGEGALTEPLLARLGTLTAVELDRDLAPRLQERFAPALRVIE
GDALALDPAELAPAQGRLRVVGNLPYNVSTPILFHLLAAADVIEDLHLLLQREVVDRMVAPPGGKTRGRLSVMVQYRCRV
ERCFNVPAGAFFPAPKVMSSFVRLVPHRPLPARAQDEAWLQEVVTAAFGGRRKTLRNSLKGLVTAQAFEAAGVDPGARAE
TLSVEAFVRLADAARSAPAAAAGR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=31.9148936170213, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI7657198, Length=226, Percent_Identity=34.5132743362832, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=50.1915708812261, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI25146882, Length=188, Percent_Identity=34.5744680851064, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25141369, Length=284, Percent_Identity=27.112676056338, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=197, Percent_Identity=31.4720812182741, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21358017, Length=220, Percent_Identity=38.1818181818182, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21357273, Length=276, Percent_Identity=31.1594202898551, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_HALHL (A1WVT7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002601.1
- ProteinModelPortal:   A1WVT7
- SMR:   A1WVT7
- STRING:   A1WVT7
- GeneID:   4709217
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1023
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- PhylomeDB:   A1WVT7
- BioCyc:   HHAL349124:HHAL_1023-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 28711; Mature: 28579

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 14-14 BINDING 39-39 BINDING 60-60 BINDING 83-83 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRPRKRFGQNFLRDPAVIQRMVTAIAPREGETLLEIGPGEGALTEPLLARLGTLTAVE
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHH
LDRDLAPRLQERFAPALRVIEGDALALDPAELAPAQGRLRVVGNLPYNVSTPILFHLLAA
HCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHH
ADVIEDLHLLLQREVVDRMVAPPGGKTRGRLSVMVQYRCRVERCFNVPAGAFFPAPKVMS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
SFVRLVPHRPLPARAQDEAWLQEVVTAAFGGRRKTLRNSLKGLVTAQAFEAAGVDPGARA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ETLSVEAFVRLADAARSAPAAAAGR
HHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SHRPRKRFGQNFLRDPAVIQRMVTAIAPREGETLLEIGPGEGALTEPLLARLGTLTAVE
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHH
LDRDLAPRLQERFAPALRVIEGDALALDPAELAPAQGRLRVVGNLPYNVSTPILFHLLAA
HCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHH
ADVIEDLHLLLQREVVDRMVAPPGGKTRGRLSVMVQYRCRVERCFNVPAGAFFPAPKVMS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
SFVRLVPHRPLPARAQDEAWLQEVVTAAFGGRRKTLRNSLKGLVTAQAFEAAGVDPGARA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ETLSVEAFVRLADAARSAPAAAAGR
HHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA