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The map label for this gene is prs [H]

Identifier: 121997780

GI number: 121997780

Start: 1061172

End: 1062128

Strand: Reverse

Name: prs [H]

Synonym: Hhal_0989

Alternate gene names: 121997780

Gene position: 1062128-1061172 (Counterclockwise)

Preceding gene: 121997781

Following gene: 121997779

Centisome position: 39.65

GC content: 65.31

Gene sequence:

>957_bases
GTGCAAGTAAACGGACCCATGATGGTCTTTGCGGGGAACTCCAACCCGCAACTGGCCGAGGCCATTGCTGCCCATCTGAA
GATCCGCCTGGGGCATGCGGTGGTCGGTCGCTTCAGCGACGACGAGATCATGGTGGAGGTCAACGAGCACGTTCGCGGCA
AAGACGTGTTCGTGGTCCAGTCCACCTGCCAGCCGGCCAACGACAACCTCATGGAATTGCTGGCCATCGTTGATGCGCTG
CGCCGTTCCTCGGCAGCGCGCGTCACGGCGGTGATCCCCTACTTCGGCTATGCCCGGCAGGACCGGCGGCCGCGGTCCCA
GCGCGTGCCCATCTCGGCCAAGCTGGTGGCCGACATGATGCAGGCCGCCGGCTACGACCGGGTGGTGACAGTCGATCTGC
ACGCCGACCAGATCCAGGGCTTCTTCAACATCCCCGTGGACAACGTGTACGCCTCGCCGATCCTGCTCGGCGACATCTGG
CGGCAGCTCTATCCACGCAAGGTCGTGGTCTCGCCGGACGTCGGCGGCGTGGTGCGGGCGCGGGCGGTGGCCAAGCGGCT
CGACGATGCCGAGCTGGCCATCATCGACAAGCGCCGTCCGCGGCCCAACGAGTCCTCGATCATGAACATCATCGGCGATG
TCCAGGACAAGACCTGCATCATCGTCGACGACATCGTGGATACCGCCGGCACCCTCTGCCAGGCTGCCGGGGCCCTTAAG
GAGCGGGGCGCACGCAAGGTGGTCGCGTACATCACCCACCCGGTGCTCTCGGGCAGCGCAGTGGACAAGATCGCGGCCTC
CGAGCTCGACGAGCTGGTGGTCAGTGACAGCATCCCGCTCGGCGAGCAAGCGGCCGCGTGCCCGAAGATTCGACAACTGA
GTATCGCGGAGATCCTCGCCGAGACGATGCGTCGGGTCAGCAATGACGAGTCGGTGAGCGAGCTCTTCGTAGAGTGA

Upstream 100 bases:

>100_bases
GCGGTAAGGCACCGGTTTTTGGTACCGGGATTCGCAGGTTCGAATCCTGCCGCCCCAGCCATATCCGCTACACGCCGAGT
TGTAAGGTGGACGTCTAGCC

Downstream 100 bases:

>100_bases
TTCCCAACCAGGAGTGGAACCAATCATGACGGTGGAAATGAGGCTCGACGCGCATCCGCGCACGGAGACCGGCCGCGGCG
CCATGCGCCGTCTGCGTCGT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MQVNGPMMVFAGNSNPQLAEAIAAHLKIRLGHAVVGRFSDDEIMVEVNEHVRGKDVFVVQSTCQPANDNLMELLAIVDAL
RRSSAARVTAVIPYFGYARQDRRPRSQRVPISAKLVADMMQAAGYDRVVTVDLHADQIQGFFNIPVDNVYASPILLGDIW
RQLYPRKVVVSPDVGGVVRARAVAKRLDDAELAIIDKRRPRPNESSIMNIIGDVQDKTCIIVDDIVDTAGTLCQAAGALK
ERGARKVVAYITHPVLSGSAVDKIAASELDELVVSDSIPLGEQAAACPKIRQLSIAEILAETMRRVSNDESVSELFVE

Sequences:

>Translated_318_residues
MQVNGPMMVFAGNSNPQLAEAIAAHLKIRLGHAVVGRFSDDEIMVEVNEHVRGKDVFVVQSTCQPANDNLMELLAIVDAL
RRSSAARVTAVIPYFGYARQDRRPRSQRVPISAKLVADMMQAAGYDRVVTVDLHADQIQGFFNIPVDNVYASPILLGDIW
RQLYPRKVVVSPDVGGVVRARAVAKRLDDAELAIIDKRRPRPNESSIMNIIGDVQDKTCIIVDDIVDTAGTLCQAAGALK
ERGARKVVAYITHPVLSGSAVDKIAASELDELVVSDSIPLGEQAAACPKIRQLSIAEILAETMRRVSNDESVSELFVE
>Mature_318_residues
MQVNGPMMVFAGNSNPQLAEAIAAHLKIRLGHAVVGRFSDDEIMVEVNEHVRGKDVFVVQSTCQPANDNLMELLAIVDAL
RRSSAARVTAVIPYFGYARQDRRPRSQRVPISAKLVADMMQAAGYDRVVTVDLHADQIQGFFNIPVDNVYASPILLGDIW
RQLYPRKVVVSPDVGGVVRARAVAKRLDDAELAIIDKRRPRPNESSIMNIIGDVQDKTCIIVDDIVDTAGTLCQAAGALK
ERGARKVVAYITHPVLSGSAVDKIAASELDELVVSDSIPLGEQAAACPKIRQLSIAEILAETMRRVSNDESVSELFVE

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=312, Percent_Identity=47.1153846153846, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=47.7707006369427, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=46.4968152866242, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI28557709, Length=310, Percent_Identity=47.0967741935484, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI4506133, Length=349, Percent_Identity=37.8223495702006, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=36.5497076023392, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI310128524, Length=149, Percent_Identity=32.8859060402685, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310115209, Length=149, Percent_Identity=32.8859060402685, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310118259, Length=149, Percent_Identity=32.8859060402685, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI310119946, Length=149, Percent_Identity=32.8859060402685, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1787458, Length=310, Percent_Identity=60.6451612903226, Blast_Score=407, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=47.0967741935484, Blast_Score=287, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI25149168, Length=310, Percent_Identity=47.0967741935484, Blast_Score=287, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17554702, Length=310, Percent_Identity=47.0967741935484, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI71989924, Length=310, Percent_Identity=47.0967741935484, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=35.5029585798817, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321776, Length=301, Percent_Identity=46.5116279069767, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6319403, Length=322, Percent_Identity=44.4099378881988, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6320946, Length=313, Percent_Identity=42.4920127795527, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324511, Length=271, Percent_Identity=32.8413284132841, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=40.6091370558376, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21355239, Length=310, Percent_Identity=48.7096774193548, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI45551540, Length=332, Percent_Identity=45.1807228915663, Blast_Score=278, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=34.6478873239437, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=34.6478873239437, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=34.6478873239437, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=34.6478873239437, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24651462, Length=373, Percent_Identity=32.4396782841823, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24651464, Length=373, Percent_Identity=32.4396782841823, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45552010, Length=373, Percent_Identity=32.4396782841823, Blast_Score=190, Evalue=9e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34662; Mature: 34662

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVNGPMMVFAGNSNPQLAEAIAAHLKIRLGHAVVGRFSDDEIMVEVNEHVRGKDVFVVQ
CCCCCCEEEEECCCCCHHHHHHHHHHHHEECHHEECCCCCCEEEEEECCCCCCCEEEEEE
STCQPANDNLMELLAIVDALRRSSAARVTAVIPYFGYARQDRRPRSQRVPISAKLVADMM
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHCCCHHHHHHHHHH
QAAGYDRVVTVDLHADQIQGFFNIPVDNVYASPILLGDIWRQLYPRKVVVSPDVGGVVRA
HHCCCCEEEEEEECHHHHCCEECCCHHHHHCCHHHHHHHHHHHCCCEEEECCCCCHHHHH
RAVAKRLDDAELAIIDKRRPRPNESSIMNIIGDVQDKTCIIVDDIVDTAGTLCQAAGALK
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
ERGARKVVAYITHPVLSGSAVDKIAASELDELVVSDSIPLGEQAAACPKIRQLSIAEILA
HCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHH
ETMRRVSNDESVSELFVE
HHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MQVNGPMMVFAGNSNPQLAEAIAAHLKIRLGHAVVGRFSDDEIMVEVNEHVRGKDVFVVQ
CCCCCCEEEEECCCCCHHHHHHHHHHHHEECHHEECCCCCCEEEEEECCCCCCCEEEEEE
STCQPANDNLMELLAIVDALRRSSAARVTAVIPYFGYARQDRRPRSQRVPISAKLVADMM
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHCCCHHHHHHHHHH
QAAGYDRVVTVDLHADQIQGFFNIPVDNVYASPILLGDIWRQLYPRKVVVSPDVGGVVRA
HHCCCCEEEEEEECHHHHCCEECCCHHHHHCCHHHHHHHHHHHCCCEEEECCCCCHHHHH
RAVAKRLDDAELAIIDKRRPRPNESSIMNIIGDVQDKTCIIVDDIVDTAGTLCQAAGALK
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
ERGARKVVAYITHPVLSGSAVDKIAASELDELVVSDSIPLGEQAAACPKIRQLSIAEILA
HCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHH
ETMRRVSNDESVSELFVE
HHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]