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The map label for this gene is tuf1 [H]

Identifier: 121997663

GI number: 121997663

Start: 944626

End: 945816

Strand: Reverse

Name: tuf1 [H]

Synonym: Hhal_0872

Alternate gene names: 121997663

Gene position: 945816-944626 (Counterclockwise)

Preceding gene: 121997664

Following gene: 121997662

Centisome position: 35.31

GC content: 66.41

Gene sequence:

>1191_bases
ATGTCCAAGGAAAAGTTCGAGCGCAAGAAGCCGCACATCAACGTCGGCACCATCGGTCACGTCGACCATGGCAAGACGAC
GCTGACTGCGGCCCTGACCAAGGTGCTCGCCGAGGCCCACGGCGGCGACGCCCGTGCGTTCGATCAGATCGACAACGCGC
CGGAGGAGCGCGCCCGCGGCATCACGATTGCCACGGCGCACGTGGAGTACGAGTCGGAGTCCCGTCACTACGCCCACGTT
GACTGCCCGGGTCACGCCGACTACGTCAAGAACATGATCACCGGCGCGGCGCAGATGGACGGCTCGATCCTGGTGGTCTC
CGCCGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTCGCCCGTCAGGTCGGCGTCCCGGCGATCGTGGTCT
TCCTGAACAAGGCCGACATGGTCGACGACGCCGAGCTGCTCGAGCTCGTCGAGATGGAGGTCCGGGAGCTGCTCAGCGAC
TACGACTTCGACGGCGACAACATCCCGGTGGTCACCGGCTCGGCGCTCAAGGCGCTCGAGGGCGACGACTCGGAGATGGG
GCGTCCGGCGATCATCAAGCTCGTCGAGGCGATGGACGCGCACATCCCGCAGCCGGAGCGTCCGGTGGATGGCGACTTCC
TCATGCCCATTGAGGACGTCTTCTCCATCTCCGGCCGCGGCACGGTGGTGACCGGTCGTGTCGAGCGCGGTGTCATCAAG
GTCGGTGAAGAGGTCGAGATCGTCGGCATCACCGACACGCGCAAGACGACCTGCACCGGCGTCGAGATGTTCCGCAAGCT
GCTCGACCAGGGTGAGGCCGGTGATAACATCGGCGCGCTGCTGCGTGGCATCAAGCGCGACGACGTCGAGCGCGGCCAGG
TGCTGTGCAAGCCGAAGTCGATCACGCCGCACACGCACTTCGAGGCCGAGGTCTACGTCCTGAGCAAGGACGAGGGTGGC
CGGCACACGCCGTTCTTCAACGGCTACCGTCCGCAGTTCTACTTCCGGACGACGGACGTCACGGGTACGGTGACGCTGCC
GGAGGGCACCGAGATGGTGATGCCGGGCGACAACGTCAAGATGACGGTTCAGCTGATCGCCCCGATCGCCATGGAGGACG
GCCTGCGCTTCGCGATTCGCGAGGGTGGCCGCACCGTCGGCGCCGGCGTCGTCTCCAAGATCCTCGACTGA

Upstream 100 bases:

>100_bases
AAGAGGTCACCGGTTCGAGTCCGGTTATGGGCTCCATTTTCATGGCGCAACGCGCAAACCGCCCTGTCAAAAAATCAGCG
AACACGGGGAGAAGCCGTCC

Downstream 100 bases:

>100_bases
TCTTGCCAAGCCGGGGGTGTGGATGATATAACTCCGCACCCTCAGGTCATCAAACACTAGGCCAGTAGCTCAATTGGCAG
AGCAGCGGTCTCCAAAACCG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSKEKFERKKPHINVGTIGHVDHGKTTLTAALTKVLAEAHGGDARAFDQIDNAPEERARGITIATAHVEYESESRHYAHV
DCPGHADYVKNMITGAAQMDGSILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKADMVDDAELLELVEMEVRELLSD
YDFDGDNIPVVTGSALKALEGDDSEMGRPAIIKLVEAMDAHIPQPERPVDGDFLMPIEDVFSISGRGTVVTGRVERGVIK
VGEEVEIVGITDTRKTTCTGVEMFRKLLDQGEAGDNIGALLRGIKRDDVERGQVLCKPKSITPHTHFEAEVYVLSKDEGG
RHTPFFNGYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNVKMTVQLIAPIAMEDGLRFAIREGGRTVGAGVVSKILD

Sequences:

>Translated_396_residues
MSKEKFERKKPHINVGTIGHVDHGKTTLTAALTKVLAEAHGGDARAFDQIDNAPEERARGITIATAHVEYESESRHYAHV
DCPGHADYVKNMITGAAQMDGSILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKADMVDDAELLELVEMEVRELLSD
YDFDGDNIPVVTGSALKALEGDDSEMGRPAIIKLVEAMDAHIPQPERPVDGDFLMPIEDVFSISGRGTVVTGRVERGVIK
VGEEVEIVGITDTRKTTCTGVEMFRKLLDQGEAGDNIGALLRGIKRDDVERGQVLCKPKSITPHTHFEAEVYVLSKDEGG
RHTPFFNGYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNVKMTVQLIAPIAMEDGLRFAIREGGRTVGAGVVSKILD
>Mature_395_residues
SKEKFERKKPHINVGTIGHVDHGKTTLTAALTKVLAEAHGGDARAFDQIDNAPEERARGITIATAHVEYESESRHYAHVD
CPGHADYVKNMITGAAQMDGSILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKADMVDDAELLELVEMEVRELLSDY
DFDGDNIPVVTGSALKALEGDDSEMGRPAIIKLVEAMDAHIPQPERPVDGDFLMPIEDVFSISGRGTVVTGRVERGVIKV
GEEVEIVGITDTRKTTCTGVEMFRKLLDQGEAGDNIGALLRGIKRDDVERGQVLCKPKSITPHTHFEAEVYVLSKDEGGR
HTPFFNGYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNVKMTVQLIAPIAMEDGLRFAIREGGRTVGAGVVSKILD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.5989847715736, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=28.1818181818182, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=28.1818181818182, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.3482142857143, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.0178571428571, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=29.7619047619048, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018522, Length=336, Percent_Identity=29.7619047619048, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=29.7619047619048, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI54607086, Length=349, Percent_Identity=28.080229226361, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=29.1925465838509, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI4503507, Length=280, Percent_Identity=23.9285714285714, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=32.1888412017167, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=32.1888412017167, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=79.2405063291139, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=79.2405063291139, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI2367247, Length=385, Percent_Identity=29.0909090909091, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=34.5833333333333, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789108, Length=276, Percent_Identity=29.7101449275362, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.605459057072, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=44.987146529563, Blast_Score=339, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=30.0220750551876, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=30.0220750551876, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.3410138248848, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=27.2311212814645, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=27.2311212814645, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.0969899665552, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=440, Percent_Identity=26.3636363636364, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=36.6666666666667, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=299, Percent_Identity=26.4214046822742, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=27.0386266094421, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=64.1414141414141, Blast_Score=521, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=31.0810810810811, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=31.0810810810811, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=273, Percent_Identity=28.2051282051282, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=29.1545189504373, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=286, Percent_Identity=25.1748251748252, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=136, Percent_Identity=33.0882352941176, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.7786259541985, Blast_Score=493, Evalue=1e-140,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.7786259541985, Blast_Score=493, Evalue=1e-140,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=51.2886597938144, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=29.5302013422819, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=29.5302013422819, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553807, Length=452, Percent_Identity=28.9823008849558, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45553816, Length=452, Percent_Identity=28.9823008849558, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24651721, Length=452, Percent_Identity=28.9823008849558, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17864154, Length=452, Percent_Identity=28.9823008849558, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.1475409836066, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17137380, Length=347, Percent_Identity=29.971181556196, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=26.3761467889908, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647046, Length=293, Percent_Identity=25.2559726962457, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24647048, Length=293, Percent_Identity=25.2559726962457, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=36.969696969697, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI78706572, Length=134, Percent_Identity=37.3134328358209, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43126; Mature: 42994

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKFERKKPHINVGTIGHVDHGKTTLTAALTKVLAEAHGGDARAFDQIDNAPEERARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITIATAHVEYESESRHYAHVDCPGHADYVKNMITGAAQMDGSILVVSAADGPMPQTREHI
EEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVFLNKADMVDDAELLELVEMEVRELLSDYDFDGDNIPVVTGSALKALE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHCC
GDDSEMGRPAIIKLVEAMDAHIPQPERPVDGDFLMPIEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCCCEEEECCCCCCEEE
VGEEVEIVGITDTRKTTCTGVEMFRKLLDQGEAGDNIGALLRGIKRDDVERGQVLCKPKS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCC
ITPHTHFEAEVYVLSKDEGGRHTPFFNGYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNVK
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCE
MTVQLIAPIAMEDGLRFAIREGGRTVGAGVVSKILD
EEEEEEECHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
SKEKFERKKPHINVGTIGHVDHGKTTLTAALTKVLAEAHGGDARAFDQIDNAPEERARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCC
ITIATAHVEYESESRHYAHVDCPGHADYVKNMITGAAQMDGSILVVSAADGPMPQTREHI
EEEEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVFLNKADMVDDAELLELVEMEVRELLSDYDFDGDNIPVVTGSALKALE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHCC
GDDSEMGRPAIIKLVEAMDAHIPQPERPVDGDFLMPIEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHCCCCCCEEEECCCCCCEEE
VGEEVEIVGITDTRKTTCTGVEMFRKLLDQGEAGDNIGALLRGIKRDDVERGQVLCKPKS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCC
ITPHTHFEAEVYVLSKDEGGRHTPFFNGYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNVK
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCE
MTVQLIAPIAMEDGLRFAIREGGRTVGAGVVSKILD
EEEEEEECHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA