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The map label for this gene is leuA [H]

Identifier: 121997487

GI number: 121997487

Start: 771745

End: 773295

Strand: Reverse

Name: leuA [H]

Synonym: Hhal_0690

Alternate gene names: 121997487

Gene position: 773295-771745 (Counterclockwise)

Preceding gene: 121997488

Following gene: 121997486

Centisome position: 28.87

GC content: 68.92

Gene sequence:

>1551_bases
ATGGGTGCAAGCGACCATCTCTACATCTTCGATACCACGCTGCGCGACGGGGAGCAGAGCCCCGGCGCCTCCATGACCCG
CGAGGAGAAGGTGCGCATCGCCAAGGGGCTCGAGCGCATGCGCGTCGACGTCCTCGAGGCCGGCTTCCCGGCGGCGAGCA
CGGGCGACTTCGAGAGCGTCCGCGCCGTCGCCGAGGCCATCAAGGAGAGCCGGGTCTGCGCCCTGGCGCGGGCGCGGGAT
CCGGATATCCACGCCGCCGGCGAGGCCCTGGCCCAGGCCGAGGCCGGGCGCATCCACACCTTCATCGCCACCTCGCCGAT
CCACATGCAGAACAAGCTGCACATGACACCGGATCAGGTGGTGGATGCGGCGGTGCATGCGGTGAGTCTGGCGCGGCAGC
TCTGCGACGACGTGGAGTTCTCGCCGGAGGACGCCGGGCGCTCCGAGCACGACTTCCTCTGCCGGATCATCGAGGCGGCC
ATCGACGCCGGGGCGCGGACCATCAACATCCCGGATACGGTGGGCTACAACCTGCCGGAGCAGTTCGGTGGGCTGATCCG
CACCCTGCGCGAGCGCATCCCCAACTCGGACAAGGCGGTGTTCTCGGTGCACTGCCACAACGACCTGGGCGTGGCGGTGG
CCAACTCCCTGGCCGCGGTCCAGGCCGGGGCGCGGCAGGTGGAGTGCACCATCAACGGCCTAGGCGAGCGGGCCGGTAAC
GCCGCCCTCGAGGAGGTGGTCATGGCGGTGAAGACCCGCCAGGACGTCTTCTCCTGCTCGACGCGGATCAACGCCCGCGA
GATCGTGCCCACCTCCAAGCTGGTGGCCAACATCACCGGCTTCCACGTCCAGCCGAACAAGGCCATCGTCGGCGTCAACG
CCTTCGCCCACGAGTCGGGCATCCACCAGGACGGGGTGCTCAAGCACCGCGAGACCTACGAGATCATGCGCGCCGAGGAC
GTCGGCTGGCACACCAACCGCATGGTCCTGGGCAAGCACTCCGGGCGGAACGCCTTCCGCTCGCGGCTCGAGGAGCTGGA
TATCCACCTCGACTCCGAGGAGCAGCTCAACGAGGCCTTCGCCCGCTTCAAGACCCTGGCCGACAAGAAGCACGAGATCT
TCGACGAGGACCTGCAGGCGCTGGTCACCGAGGCCGGTGCGGCGCTGGAGAACGAGCGCATCCGGCTGCTCGCGCTGCAC
GTCTGCTCGGAGACCGGGGAGCAGCCGGAGGCCACCGTGTCGCTGCTGATCGACGGCGAGGAACGTCAGGCGGTGGCCCC
GGGCAGCGGCGCGGTGGATGCGGCGTTCATGGCGGTGGACTCGCTGGTGCAGTCGGGCACCGAGCTGTTGCTCTACTCGG
TGAACAACATCACCTCCGGGACCGACTCCCAGGGCGAGGTCACCGTGCGTCTGGAGCGCGGCGGCCGGGTGGCCAACGGG
CAGGGCGCCGATGTGGACATCGTCATCGCCTCGGCGAAGGCGTATATCAACGCCCTGAACAAGATCCTCGCCTCGCCGGA
TCGGCGCCACCCGCAGGCCGGAGACGTCTAA

Upstream 100 bases:

>100_bases
CGAACATCCCACCCCACCGCTTCTGCACGGGCGCCCCGGCGGCGCCGTGGACGACTTCCATTCGGCGGTGCTGACCGCCA
CGACACAGGGCTGAGCAGCC

Downstream 100 bases:

>100_bases
GGCGTGGATACCCGGCGGCTCAGCTACCTGCGCCAACTGGGGGTGGACTGCTACATCCCCCGCGACGCCGCCGTGCTGGA
GCCGGCGGCGCCCCGGCAGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MGASDHLYIFDTTLRDGEQSPGASMTREEKVRIAKGLERMRVDVLEAGFPAASTGDFESVRAVAEAIKESRVCALARARD
PDIHAAGEALAQAEAGRIHTFIATSPIHMQNKLHMTPDQVVDAAVHAVSLARQLCDDVEFSPEDAGRSEHDFLCRIIEAA
IDAGARTINIPDTVGYNLPEQFGGLIRTLRERIPNSDKAVFSVHCHNDLGVAVANSLAAVQAGARQVECTINGLGERAGN
AALEEVVMAVKTRQDVFSCSTRINAREIVPTSKLVANITGFHVQPNKAIVGVNAFAHESGIHQDGVLKHRETYEIMRAED
VGWHTNRMVLGKHSGRNAFRSRLEELDIHLDSEEQLNEAFARFKTLADKKHEIFDEDLQALVTEAGAALENERIRLLALH
VCSETGEQPEATVSLLIDGEERQAVAPGSGAVDAAFMAVDSLVQSGTELLLYSVNNITSGTDSQGEVTVRLERGGRVANG
QGADVDIVIASAKAYINALNKILASPDRRHPQAGDV

Sequences:

>Translated_516_residues
MGASDHLYIFDTTLRDGEQSPGASMTREEKVRIAKGLERMRVDVLEAGFPAASTGDFESVRAVAEAIKESRVCALARARD
PDIHAAGEALAQAEAGRIHTFIATSPIHMQNKLHMTPDQVVDAAVHAVSLARQLCDDVEFSPEDAGRSEHDFLCRIIEAA
IDAGARTINIPDTVGYNLPEQFGGLIRTLRERIPNSDKAVFSVHCHNDLGVAVANSLAAVQAGARQVECTINGLGERAGN
AALEEVVMAVKTRQDVFSCSTRINAREIVPTSKLVANITGFHVQPNKAIVGVNAFAHESGIHQDGVLKHRETYEIMRAED
VGWHTNRMVLGKHSGRNAFRSRLEELDIHLDSEEQLNEAFARFKTLADKKHEIFDEDLQALVTEAGAALENERIRLLALH
VCSETGEQPEATVSLLIDGEERQAVAPGSGAVDAAFMAVDSLVQSGTELLLYSVNNITSGTDSQGEVTVRLERGGRVANG
QGADVDIVIASAKAYINALNKILASPDRRHPQAGDV
>Mature_515_residues
GASDHLYIFDTTLRDGEQSPGASMTREEKVRIAKGLERMRVDVLEAGFPAASTGDFESVRAVAEAIKESRVCALARARDP
DIHAAGEALAQAEAGRIHTFIATSPIHMQNKLHMTPDQVVDAAVHAVSLARQLCDDVEFSPEDAGRSEHDFLCRIIEAAI
DAGARTINIPDTVGYNLPEQFGGLIRTLRERIPNSDKAVFSVHCHNDLGVAVANSLAAVQAGARQVECTINGLGERAGNA
ALEEVVMAVKTRQDVFSCSTRINAREIVPTSKLVANITGFHVQPNKAIVGVNAFAHESGIHQDGVLKHRETYEIMRAEDV
GWHTNRMVLGKHSGRNAFRSRLEELDIHLDSEEQLNEAFARFKTLADKKHEIFDEDLQALVTEAGAALENERIRLLALHV
CSETGEQPEATVSLLIDGEERQAVAPGSGAVDAAFMAVDSLVQSGTELLLYSVNNITSGTDSQGEVTVRLERGGRVANGQ
GADVDIVIASAKAYINALNKILASPDRRHPQAGDV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=500, Percent_Identity=50.8, Blast_Score=474, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320071, Length=392, Percent_Identity=28.0612244897959, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=28.4153005464481, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324682, Length=541, Percent_Identity=25.8780036968577, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=559, Percent_Identity=26.2969588550984, Blast_Score=119, Evalue=9e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55718; Mature: 55587

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGASDHLYIFDTTLRDGEQSPGASMTREEKVRIAKGLERMRVDVLEAGFPAASTGDFESV
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RAVAEAIKESRVCALARARDPDIHAAGEALAQAEAGRIHTFIATSPIHMQNKLHMTPDQV
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCHHHH
VDAAVHAVSLARQLCDDVEFSPEDAGRSEHDFLCRIIEAAIDAGARTINIPDTVGYNLPE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
QFGGLIRTLRERIPNSDKAVFSVHCHNDLGVAVANSLAAVQAGARQVECTINGLGERAGN
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHCCH
AALEEVVMAVKTRQDVFSCSTRINAREIVPTSKLVANITGFHVQPNKAIVGVNAFAHESG
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCEEECCCCEEEEEEHHHHHCC
IHQDGVLKHRETYEIMRAEDVGWHTNRMVLGKHSGRNAFRSRLEELDIHLDSEEQLNEAF
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHH
ARFKTLADKKHEIFDEDLQALVTEAGAALENERIRLLALHVCSETGEQPEATVSLLIDGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEEEEHHCCCCCCCCEEEEEEECC
ERQAVAPGSGAVDAAFMAVDSLVQSGTELLLYSVNNITSGTDSQGEVTVRLERGGRVANG
CCCEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEECCC
QGADVDIVIASAKAYINALNKILASPDRRHPQAGDV
CCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GASDHLYIFDTTLRDGEQSPGASMTREEKVRIAKGLERMRVDVLEAGFPAASTGDFESV
CCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RAVAEAIKESRVCALARARDPDIHAAGEALAQAEAGRIHTFIATSPIHMQNKLHMTPDQV
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCHHHH
VDAAVHAVSLARQLCDDVEFSPEDAGRSEHDFLCRIIEAAIDAGARTINIPDTVGYNLPE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHH
QFGGLIRTLRERIPNSDKAVFSVHCHNDLGVAVANSLAAVQAGARQVECTINGLGERAGN
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHCCH
AALEEVVMAVKTRQDVFSCSTRINAREIVPTSKLVANITGFHVQPNKAIVGVNAFAHESG
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHCCCEEECCCCEEEEEEHHHHHCC
IHQDGVLKHRETYEIMRAEDVGWHTNRMVLGKHSGRNAFRSRLEELDIHLDSEEQLNEAF
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHH
ARFKTLADKKHEIFDEDLQALVTEAGAALENERIRLLALHVCSETGEQPEATVSLLIDGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEEEEEEEHHCCCCCCCCEEEEEEECC
ERQAVAPGSGAVDAAFMAVDSLVQSGTELLLYSVNNITSGTDSQGEVTVRLERGGRVANG
CCCEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEECCC
QGADVDIVIASAKAYINALNKILASPDRRHPQAGDV
CCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA