Click here to switch to the map view.

The map label for this gene is pcnB [H]

Identifier: 121997474

GI number: 121997474

Start: 759376

End: 760611

Strand: Reverse

Name: pcnB [H]

Synonym: Hhal_0677

Alternate gene names: 121997474

Gene position: 760611-759376 (Counterclockwise)

Preceding gene: 121997476

Following gene: 121997473

Centisome position: 28.4

GC content: 70.47

Gene sequence:

>1236_bases
ATGGTGGGCGGCGGGGTGCGCGACCTCTCGCTCGGGCGGGAGCCGAAGGACTTCGACGTGGCCACGGATGCCCGGCCCGA
GGAGGTGCGTAAGCTCTTTCGCAACTGCCGGCTCATTGGCCGGCGCTTCCGCCTCGCCCACGTCCACTTCGGGGCCGAGA
TCATCGAGGTGGCCACCTTCCGTGCGCTGCATCCGCCGGAAGACAGCGAGGGCGACGACGCCGACGGCGAGCGGGTGATC
GAGAACGGCCGCATCGTCCGCGACAACGTCTACGGCACGCTGGCCGAGGACGCCCTGCGCCGCGATTTCACCGTCAACGC
TCTGTACTACAACATCGATGATTTCTCCGTGGTCGACCACGCCGGCGGCATGGACGACCTGGAGGACGGCATCATCCGTC
TGATCGGTGATCCGCACGAACGCTACCAGGAAGACCCGGTGCGCATGCTGCGCGCCGTGCGCTTCGCCCTGAAGCTCGGC
TTCCGCATCCATCCGCAGACGGCGGCGCCGATGCACGATATGGCCGACAGCGTCGCCGACATGCCCCCGGCGCGGCTCTT
CGATGAGGCCCTGAAGCTGTTCATGTCCGGCGATGCGCTGGACGCCTTCGAGATGCTGCGCCACTACGGGCTGTTCGAGT
CGCTGCTGCCGCTGACCGCCGAAGCGCTGGAGGAAGAGGGCAGTGAGCAGTGGGTGCCCTTCATCGCCGCAGCGCTGCGT
AATACCGACGAGCGCATCGGCGCCGACAAGCCGGTGACCCCGGCCTTCCTCTTCGCCGCGCTGCTCTGGCCGGCGGTCCG
TCAGCGCGAGGCCGAGCTGGTCGAGGACGGCGAGGCGCCGATCCCGGCGCTGCAGCAGGCGGCTGCCGAGGTGCAGACCC
TGCAGCAGTCCCGCATCGCACTGCCCAAGCGCTTCAGTATCCCCATGCGCGAGATCTGGACCCTGCAGCCGCGCTTCGCG
CGCCGCGGGGGCAAGCGGGCGCAGCGGCTGCTCGGGCATCCGCGCTTCCGCGCCGCCTACGACTTCCTCGAGCTGCGCGC
CGGGGCCGGTGACGCCCCCGCCGAGCTGGCGCAGTGGTGGCGCGAGTTCCAGGAGCAGCCCGAGCGGCCGCCGTCCCAGA
AGAAGAGCAGCCGTGGCGGCCGGCGCCGGCGCGGTGGTCGGGGGCGTGGCAAAGGGAGCGAAGATGGCGATCGCGCCGAT
ACGGCGGCGTCGAGCCCGGAGTCCTCGGCGGAATGA

Upstream 100 bases:

>100_bases
CATCATCCCCCGGGATGCTCACAGCATCTCGCGCGCGGACATCAGCGATAACGCCCTGAAGGTCCTCTACCGTCTGCGCA
ACGCCGGCTACCAGGCCTAC

Downstream 100 bases:

>100_bases
GCACGGCGATCACCGCCTATGTGGGACTCGGCAGCAACCTCGATCAGCCGCGGCGGCAGATCGAGCGGGCCGTGGTGCGC
CTGGATCTGCTCCCCGGTTG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MVGGGVRDLSLGREPKDFDVATDARPEEVRKLFRNCRLIGRRFRLAHVHFGAEIIEVATFRALHPPEDSEGDDADGERVI
ENGRIVRDNVYGTLAEDALRRDFTVNALYYNIDDFSVVDHAGGMDDLEDGIIRLIGDPHERYQEDPVRMLRAVRFALKLG
FRIHPQTAAPMHDMADSVADMPPARLFDEALKLFMSGDALDAFEMLRHYGLFESLLPLTAEALEEEGSEQWVPFIAAALR
NTDERIGADKPVTPAFLFAALLWPAVRQREAELVEDGEAPIPALQQAAAEVQTLQQSRIALPKRFSIPMREIWTLQPRFA
RRGGKRAQRLLGHPRFRAAYDFLELRAGAGDAPAELAQWWREFQEQPERPPSQKKSSRGGRRRRGGRGRGKGSEDGDRAD
TAASSPESSAE

Sequences:

>Translated_411_residues
MVGGGVRDLSLGREPKDFDVATDARPEEVRKLFRNCRLIGRRFRLAHVHFGAEIIEVATFRALHPPEDSEGDDADGERVI
ENGRIVRDNVYGTLAEDALRRDFTVNALYYNIDDFSVVDHAGGMDDLEDGIIRLIGDPHERYQEDPVRMLRAVRFALKLG
FRIHPQTAAPMHDMADSVADMPPARLFDEALKLFMSGDALDAFEMLRHYGLFESLLPLTAEALEEEGSEQWVPFIAAALR
NTDERIGADKPVTPAFLFAALLWPAVRQREAELVEDGEAPIPALQQAAAEVQTLQQSRIALPKRFSIPMREIWTLQPRFA
RRGGKRAQRLLGHPRFRAAYDFLELRAGAGDAPAELAQWWREFQEQPERPPSQKKSSRGGRRRRGGRGRGKGSEDGDRAD
TAASSPESSAE
>Mature_411_residues
MVGGGVRDLSLGREPKDFDVATDARPEEVRKLFRNCRLIGRRFRLAHVHFGAEIIEVATFRALHPPEDSEGDDADGERVI
ENGRIVRDNVYGTLAEDALRRDFTVNALYYNIDDFSVVDHAGGMDDLEDGIIRLIGDPHERYQEDPVRMLRAVRFALKLG
FRIHPQTAAPMHDMADSVADMPPARLFDEALKLFMSGDALDAFEMLRHYGLFESLLPLTAEALEEEGSEQWVPFIAAALR
NTDERIGADKPVTPAFLFAALLWPAVRQREAELVEDGEAPIPALQQAAAEVQTLQQSRIALPKRFSIPMREIWTLQPRFA
RRGGKRAQRLLGHPRFRAAYDFLELRAGAGDAPAELAQWWREFQEQPERPPSQKKSSRGGRRRRGGRGRGKGSEDGDRAD
TAASSPESSAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=209, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI87081691, Length=378, Percent_Identity=52.6455026455027, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1789436, Length=286, Percent_Identity=29.020979020979, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=273, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321016, Length=203, Percent_Identity=30.5418719211823, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 46087; Mature: 46087

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGGGVRDLSLGREPKDFDVATDARPEEVRKLFRNCRLIGRRFRLAHVHFGAEIIEVATF
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALHPPEDSEGDDADGERVIENGRIVRDNVYGTLAEDALRRDFTVNALYYNIDDFSVVDH
HCCCCCCCCCCCCCCCHHHHHCCCEEECCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHC
AGGMDDLEDGIIRLIGDPHERYQEDPVRMLRAVRFALKLGFRIHPQTAAPMHDMADSVAD
CCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHC
MPPARLFDEALKLFMSGDALDAFEMLRHYGLFESLLPLTAEALEEEGSEQWVPFIAAALR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NTDERIGADKPVTPAFLFAALLWPAVRQREAELVEDGEAPIPALQQAAAEVQTLQQSRIA
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
LPKRFSIPMREIWTLQPRFARRGGKRAQRLLGHPRFRAAYDFLELRAGAGDAPAELAQWW
CCCCCCCCHHHHHCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
REFQEQPERPPSQKKSSRGGRRRRGGRGRGKGSEDGDRADTAASSPESSAE
HHHHHCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MVGGGVRDLSLGREPKDFDVATDARPEEVRKLFRNCRLIGRRFRLAHVHFGAEIIEVATF
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALHPPEDSEGDDADGERVIENGRIVRDNVYGTLAEDALRRDFTVNALYYNIDDFSVVDH
HCCCCCCCCCCCCCCCHHHHHCCCEEECCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHC
AGGMDDLEDGIIRLIGDPHERYQEDPVRMLRAVRFALKLGFRIHPQTAAPMHDMADSVAD
CCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHC
MPPARLFDEALKLFMSGDALDAFEMLRHYGLFESLLPLTAEALEEEGSEQWVPFIAAALR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NTDERIGADKPVTPAFLFAALLWPAVRQREAELVEDGEAPIPALQQAAAEVQTLQQSRIA
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
LPKRFSIPMREIWTLQPRFARRGGKRAQRLLGHPRFRAAYDFLELRAGAGDAPAELAQWW
CCCCCCCCHHHHHCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
REFQEQPERPPSQKKSSRGGRRRRGGRGRGKGSEDGDRADTAASSPESSAE
HHHHHCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]