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The map label for this gene is purA

Identifier: 121997457

GI number: 121997457

Start: 742701

End: 743996

Strand: Reverse

Name: purA

Synonym: Hhal_0660

Alternate gene names: 121997457

Gene position: 743996-742701 (Counterclockwise)

Preceding gene: 121997458

Following gene: 121997456

Centisome position: 27.78

GC content: 68.98

Gene sequence:

>1296_bases
ATGGGCACCAATGTGGTCGTAGTCGGTACGCAGTGGGGCGACGAAGGCAAGGGCAAGATCGTCGACTGGCTGACCGAGAA
CGCCGGGGTGGTGGCGCGCTTTCAGGGCGGGCACAACGCGGGGCATACCCTGGTCATCGACGGCGAGCAGACGGTGCTGC
ACCTGATCCCGTCCGGCATCCTGCGCGAGGACGCCACCTGCGTGATCGGCAACGGCGTGGTCCTCTCCGCCGAGGCGCTG
CTCGAGGAGATCCGTGAGCTGGAGGCCCGCGGCGTTCCGGCCCGGGAGCGGCTGCGCATCAGTCCCTCCTGCCCGCTGAT
CCTGCCCTGCCACGTGGCGCTGGATCACGCCCGGGAAAAGGCCAAGGGCAAGGCGGCCATCGGCACCACCGGTCGCGGCA
TCGGCCCGGCCTATGAGGACAAGGTGGCCCGGCGCGGCGTCCGGCTCTCGGACCTGTTCCACCGTGAGCGTCTGGCGCAG
AAGCTCGGCGAGCTGCTCGACTACCACAACTTCGTGCTCAAGCACTACTTCGGTGCCCCGACCATGGACTTCCAGGAAGT
CCTCGATCAGGCGCTGGCCCACGGCGAGGAACTGCGGCCGCTGACCGCCGACGTCGGCGCGCTGCTGACCGCCGAGATGC
GCACCGGGCGCAACATCCTCTTCGAGGGGGCGCAGGGGACGCTGCTCGACATCGATCACGGCACCTATCCGTTCGTGACC
TCTTCGAACACCTGCGCCGGGGCGGCGGCCGCCGGTACCGGGGTTGGGCCGCGCACTCTGGACTACATCCTGGGGATCAC
CAAGGCGTACACCACGCGGGTGGGGCAGGGGCCGTTCCCGACCGAGCTCGATTTCGACGACGAGATGGGCATGCACTTGG
GCGAGCGGGGCCACGAGTTCGGGGCCACCACCGGCCGGCAGCGGCGCTGCGGGTGGTTCGACGCCGTGGCCACGCGCCGG
GCGGCGCAGATCAACAGCCTCAGCGGGCTGTGCATCACCAAGCTGGACGTGCTCGACGGCCTGGAGACCCTGCGCCTGTG
CGTCGGCTACCGCTGCCAGGGCGAGCTCTGCGAGACCCTGCCGTCGGAGGTCGAGACCCTGTCCGAGTGCGAGCCGGTCT
ACGAGGACATGCCTGGCTGGGCAGAGAGCACCTCGGGCATCGAGCGCTACGAGGATCTGCCGGAGAACGCCCGCGCCTAT
TTGGGTCGTATCGAGGAGTTGGTGGGCGTGCCGGTCGCCATGATCTCTACTGGGCCCGACCGCAACGAGACCATCGTCCG
CCAGAGCCCCTTTTAA

Upstream 100 bases:

>100_bases
GGGTGGTCTGGCAGCTGCCGGGGGATGAGCAGGACAACCACGGTTGCGATCGGCGCCTGGTCGAGCGCAACGGCACCTGG
GAACTGGATACGGAGTGAGT

Downstream 100 bases:

>100_bases
AAGGAAGGCGGCGTCCGCCGCTGCCGGCTCCCGCCCCGCCGGCTGGCCCCGCGCCGGTCGGCGGTGGCGGGGTGTCTTGA
TCCGTATCCAAAAGAGAGAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGTNVVVVGTQWGDEGKGKIVDWLTENAGVVARFQGGHNAGHTLVIDGEQTVLHLIPSGILREDATCVIGNGVVLSAEAL
LEEIRELEARGVPARERLRISPSCPLILPCHVALDHAREKAKGKAAIGTTGRGIGPAYEDKVARRGVRLSDLFHRERLAQ
KLGELLDYHNFVLKHYFGAPTMDFQEVLDQALAHGEELRPLTADVGALLTAEMRTGRNILFEGAQGTLLDIDHGTYPFVT
SSNTCAGAAAAGTGVGPRTLDYILGITKAYTTRVGQGPFPTELDFDDEMGMHLGERGHEFGATTGRQRRCGWFDAVATRR
AAQINSLSGLCITKLDVLDGLETLRLCVGYRCQGELCETLPSEVETLSECEPVYEDMPGWAESTSGIERYEDLPENARAY
LGRIEELVGVPVAMISTGPDRNETIVRQSPF

Sequences:

>Translated_431_residues
MGTNVVVVGTQWGDEGKGKIVDWLTENAGVVARFQGGHNAGHTLVIDGEQTVLHLIPSGILREDATCVIGNGVVLSAEAL
LEEIRELEARGVPARERLRISPSCPLILPCHVALDHAREKAKGKAAIGTTGRGIGPAYEDKVARRGVRLSDLFHRERLAQ
KLGELLDYHNFVLKHYFGAPTMDFQEVLDQALAHGEELRPLTADVGALLTAEMRTGRNILFEGAQGTLLDIDHGTYPFVT
SSNTCAGAAAAGTGVGPRTLDYILGITKAYTTRVGQGPFPTELDFDDEMGMHLGERGHEFGATTGRQRRCGWFDAVATRR
AAQINSLSGLCITKLDVLDGLETLRLCVGYRCQGELCETLPSEVETLSECEPVYEDMPGWAESTSGIERYEDLPENARAY
LGRIEELVGVPVAMISTGPDRNETIVRQSPF
>Mature_430_residues
GTNVVVVGTQWGDEGKGKIVDWLTENAGVVARFQGGHNAGHTLVIDGEQTVLHLIPSGILREDATCVIGNGVVLSAEALL
EEIRELEARGVPARERLRISPSCPLILPCHVALDHAREKAKGKAAIGTTGRGIGPAYEDKVARRGVRLSDLFHRERLAQK
LGELLDYHNFVLKHYFGAPTMDFQEVLDQALAHGEELRPLTADVGALLTAEMRTGRNILFEGAQGTLLDIDHGTYPFVTS
SNTCAGAAAAGTGVGPRTLDYILGITKAYTTRVGQGPFPTELDFDDEMGMHLGERGHEFGATTGRQRRCGWFDAVATRRA
AQINSLSGLCITKLDVLDGLETLRLCVGYRCQGELCETLPSEVETLSECEPVYEDMPGWAESTSGIERYEDLPENARAYL
GRIEELVGVPVAMISTGPDRNETIVRQSPF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=42.3887587822014, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=40.5594405594406, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=40.9547738693467, Blast_Score=285, Evalue=7e-77,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=63.1944444444444, Blast_Score=560, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=42.1296296296296, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=42.1296296296296, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=41.9354838709677, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=40.4255319148936, Blast_Score=321, Evalue=5e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_HALHL (A1WUT0)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002244.1
- ProteinModelPortal:   A1WUT0
- SMR:   A1WUT0
- STRING:   A1WUT0
- GeneID:   4709353
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0660
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   A1WUT0
- ProtClustDB:   PRK01117
- BioCyc:   HHAL349124:HHAL_0660-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46751; Mature: 46619

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 306-306 BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTNVVVVGTQWGDEGKGKIVDWLTENAGVVARFQGGHNAGHTLVIDGEQTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEHHHCCHH
LREDATCVIGNGVVLSAEALLEEIRELEARGVPARERLRISPSCPLILPCHVALDHAREK
HCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCHHHHEEECCCCCEEEEHHHHHHHHHHH
AKGKAAIGTTGRGIGPAYEDKVARRGVRLSDLFHRERLAQKLGELLDYHNFVLKHYFGAP
HCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TMDFQEVLDQALAHGEELRPLTADVGALLTAEMRTGRNILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTCAGAAAAGTGVGPRTLDYILGITKAYTTRVGQGPFPTELDFDDEMGMHLGERGHEF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCHHHHHCCCCC
GATTGRQRRCGWFDAVATRRAAQINSLSGLCITKLDVLDGLETLRLCVGYRCQGELCETL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
PSEVETLSECEPVYEDMPGWAESTSGIERYEDLPENARAYLGRIEELVGVPVAMISTGPD
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCC
RNETIVRQSPF
CCCEEECCCCC
>Mature Secondary Structure 
GTNVVVVGTQWGDEGKGKIVDWLTENAGVVARFQGGHNAGHTLVIDGEQTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEHHHCCHH
LREDATCVIGNGVVLSAEALLEEIRELEARGVPARERLRISPSCPLILPCHVALDHAREK
HCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCHHHHEEECCCCCEEEEHHHHHHHHHHH
AKGKAAIGTTGRGIGPAYEDKVARRGVRLSDLFHRERLAQKLGELLDYHNFVLKHYFGAP
HCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TMDFQEVLDQALAHGEELRPLTADVGALLTAEMRTGRNILFEGAQGTLLDIDHGTYPFVT
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTCAGAAAAGTGVGPRTLDYILGITKAYTTRVGQGPFPTELDFDDEMGMHLGERGHEF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCHHHHHCCCCC
GATTGRQRRCGWFDAVATRRAAQINSLSGLCITKLDVLDGLETLRLCVGYRCQGELCETL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
PSEVETLSECEPVYEDMPGWAESTSGIERYEDLPENARAYLGRIEELVGVPVAMISTGPD
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCC
RNETIVRQSPF
CCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA