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The map label for this gene is sigH_1

Identifier: 121639111

GI number: 121639111

Start: 3551383

End: 3552033

Strand: Reverse

Name: sigH_1

Synonym: BCG_3251c

Alternate gene names: 121639111

Gene position: 3552033-3551383 (Counterclockwise)

Preceding gene: 121639115

Following gene: 121639110

Centisome position: 81.2

GC content: 61.6

Gene sequence:

>651_bases
ATGGCCGACATCGATGGTGTAACCGGTTCGGCGGGTCTGCAGCCTGGGCCGTCTGAGGAGACAGACGAGGAGTTGACCGC
GCGTTTCGAGCGCGACGCGATTCCCCTGTTGGACCAGCTGTACGGCGGTGCGCTGCGGATGACGCGCAATCCGGCCGACG
CCGAGGACTTGCTCCAGGAGACGATGGTGAAGGCCTATGCGGGATTTCGTTCGTTCCGGCACGGTACCAATCTCAAGGCC
TGGCTCTACCGGATACTGACCAACACCTACATCAACAGCTATCGCAAGAAACAGCGGCAACCGGCGGAGTATCCGACCGA
GCAGATCACCGATTGGCAACTGGCGTCCAACGCCGAGCATTCCTCGACCGGGCTGCGCTCGGCTGAAGTCGAAGCGTTAG
AAGCGTTGCCGGACACCGAGATCAAAGAGGCGCTGCAGGCATTGCCGGAAGAGTTCCGGATGGCGGTCTACTACGCCGAT
GTCGAAGGTTTCCCCTACAAGGAGATCGCCGAGATCATGGATACTCCGATCGGCACCGTGATGTCGAGGCTTCATCGCGG
CCGACGTCAGTTGCGCGGTCTTTTAGCCGATGTGGCCAGGGATCGGGGGTTTGCCAGGGGCGAGCAGGCGCACGAGGGGG
TGTCGTCATGA

Upstream 100 bases:

>100_bases
CCTGTTGGGTGAGAAGCGGTTGGCTCGCATGCTCGCACGTCCGGTGTCCGCGCCGGTGCTGTCCGGCGACACCGCAAACG
AAGGGACCCAGTTAATCAAG

Downstream 100 bases:

>100_bases
GCAGCCCGGTGTCAAGCCGCCGTTTGGCGAACTTGGTCAAGGAGAGCCTGCAGGGCTCGGTGTTGGGTGGGGTCGTAAGC
GATGCCGTCTTGCCAGCGGT

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MADIDGVTGSAGLQPGPSEETDEELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQETMVKAYAGFRSFRHGTNLKA
WLYRILTNTYINSYRKKQRQPAEYPTEQITDWQLASNAEHSSTGLRSAEVEALEALPDTEIKEALQALPEEFRMAVYYAD
VEGFPYKEIAEIMDTPIGTVMSRLHRGRRQLRGLLADVARDRGFARGEQAHEGVSS

Sequences:

>Translated_216_residues
MADIDGVTGSAGLQPGPSEETDEELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQETMVKAYAGFRSFRHGTNLKA
WLYRILTNTYINSYRKKQRQPAEYPTEQITDWQLASNAEHSSTGLRSAEVEALEALPDTEIKEALQALPEEFRMAVYYAD
VEGFPYKEIAEIMDTPIGTVMSRLHRGRRQLRGLLADVARDRGFARGEQAHEGVSS
>Mature_215_residues
ADIDGVTGSAGLQPGPSEETDEELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQETMVKAYAGFRSFRHGTNLKAW
LYRILTNTYINSYRKKQRQPAEYPTEQITDWQLASNAEHSSTGLRSAEVEALEALPDTEIKEALQALPEEFRMAVYYADV
EGFPYKEIAEIMDTPIGTVMSRLHRGRRQLRGLLADVARDRGFARGEQAHEGVSS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

Organism=Escherichia coli, GI1788926, Length=141, Percent_Identity=36.1702127659575, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): RPOE_MYCBO (P66808)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_856895.1
- ProteinModelPortal:   P66808
- SMR:   P66808
- PhosSite:   P66808
- EnsemblBacteria:   EBMYCT00000016375
- GeneID:   1094033
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3250c
- GeneTree:   EBGT00050000015254
- HOGENOM:   HBG753452
- OMA:   GAALRMT
- ProtClustDB:   PRK09652
- BioCyc:   MBOV233413:MB3250C-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- TIGRFAMs:   TIGR02947
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 24225; Mature: 24094

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIDGVTGSAGLQPGPSEETDEELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHH
TMVKAYAGFRSFRHGTNLKAWLYRILTNTYINSYRKKQRQPAEYPTEQITDWQLASNAEH
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCH
SSTGLRSAEVEALEALPDTEIKEALQALPEEFRMAVYYADVEGFPYKEIAEIMDTPIGTV
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCHHHH
MSRLHRGRRQLRGLLADVARDRGFARGEQAHEGVSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC
>Mature Secondary Structure 
ADIDGVTGSAGLQPGPSEETDEELTARFERDAIPLLDQLYGGALRMTRNPADAEDLLQE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHH
TMVKAYAGFRSFRHGTNLKAWLYRILTNTYINSYRKKQRQPAEYPTEQITDWQLASNAEH
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCH
SSTGLRSAEVEALEALPDTEIKEALQALPEEFRMAVYYADVEGFPYKEIAEIMDTPIGTV
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCHHHH
MSRLHRGRRQLRGLLADVARDRGFARGEQAHEGVSS
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972