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The map label for this gene is gatA [H]

Identifier: 121638893

GI number: 121638893

Start: 3324437

End: 3325921

Strand: Reverse

Name: gatA [H]

Synonym: BCG_3033c

Alternate gene names: 121638893

Gene position: 3325921-3324437 (Counterclockwise)

Preceding gene: 121638894

Following gene: 121638892

Centisome position: 76.03

GC content: 69.97

Gene sequence:

>1485_bases
GTGACGGACATCATCCGATCCGACGCCGCGACGTTGGCCGCCAAGATCGCCATCAAGGAGGTGTCGTCGACCGAGATCAC
CCGGGCCTGCCTGGATCAGATCGAGGCGACCGACGAGACGTACCACGCCTTCCTGCATGTGGCGGCCGATGAGGCGCTGG
CGGCGGCGGCCGCCGTCGACAAGCAGGTGGCCGCTGGAGAACCCTTGCCGTCGGCGCTGGCCGGGGTGCCGCTGGCGCTC
AAGGACGTCTTCACCACCAGCGACATGCCCACCACCTGCGGGTCAAAAATCCTGGAGGGATGGCGATCTCCCTACGACGC
CACGCTGACCGCGCGGTTGCGCGCCGCGGGGATCCCGATCCTGGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGT
CGACGGAGAACTCCGCTTACGGTCCCACCCGCAACCCGTGGAATCTCGACCGGGTACCCGGCGGTTCCGGTGGCGGCAGC
GCGGCGGCGCTGGCCGCGTTCCAGGCGCCGCTGGCCATCGGATCCGACACCGGGGGGTCGATCCGCCAGCCGGCCGCGCT
GACCGCGACCGTCGGCGTCAAACCCACCTACGGCACGGTGTCGCGCTATGGGCTGGTGGCCTGCGCGTCCTCGCTGGATC
AGGGCGGCCCGTGTGCGCGCACCGTCTTGGACACCGCGCTGTTGCATCAGGTGATCGCCGGCCACGACCCGCGCGACTCC
ACGTCGGTCGACGCCGAGGTGCCCGACGTGGTGGGCGCCGCTAGGGCCGGCGCGGTCGGGGATCTGCGTGGCGTGCGGGT
CGGCGTGGTTCGACAGCTGCACGGCGGCGAGGGCTACCAGCCGGGCGTGCTGGCCTCCTTCGAGGCTGCCGTGGAGCAGC
TAACCGCGCTGGGCGCTGAGGTCAGCGAGGTCGACTGCCCGCACTTCGACCATGCCCTGGCCGCCTATTACCTGATTCTG
CCCTCGGAGGTGTCGAGCAATCTGGCGCGCTTCGACGCGATGCGCTACGGGCTGCGGGTCGGCGACGACGGCACCCGCAG
CGCCGAGGAGGTGATGGCGATGACCCGGGCCGCCGGTTTCGGGCCCGAGGTCAAGCGGCGCATCATGATCGGCACCTACG
CGTTGTCGGCCGGCTACTACGACGCCTATTACAACCAGGCGCAGAAGGTGCGCACGCTGATCGCCCGCGACCTCGACGCG
GCGTATCGGTCCGTCGACGTGCTGGTGTCGCCCACGACCCCGACCACCGCGTTCCGGCTGGGTGAGAAGGTGGACGATCC
GCTGGCGATGTACTTGTTCGACCTGTGCACGCTGCCGCTGAACTTGGCCGGCCACTGCGGCATGTCTGTGCCGTCGGGGC
TCTCCCCGGACGACGGGTTGCCGGTTGGCCTACAGATCATGGCGCCGGCATTGGCCGACGACCGGCTCTACCGGGTGGGG
GCGGCTTATGAGGCCGCCCGCGGCCCGCTACTGAGCGCCATTTAG

Upstream 100 bases:

>100_bases
ACGAGACCGTGCCGTGCCTGACCCAGCGTCAGGTGCTCGACCAGGCGCCCGATGCCGTCGACGGCCGCTTCGCCGTCCCG
CAGATCCTGGGGGATGAACA

Downstream 100 bases:

>100_bases
CGGCGAGCAGACACAGACTCGCATCCGGCGCCCCTCGCCCGTGCGATTCTGTGTCTGCTCGCGCCAGGTCGGCGGCCGCG
AGGCAAGATGAGGGTATGCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTDIIRSDAATLAAKIAIKEVSSTEITRACLDQIEATDETYHAFLHVAADEALAAAAAVDKQVAAGEPLPSALAGVPLAL
KDVFTTSDMPTTCGSKILEGWRSPYDATLTARLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNLDRVPGGSGGGS
AAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDS
TSVDAEVPDVVGAARAGAVGDLRGVRVGVVRQLHGGEGYQPGVLASFEAAVEQLTALGAEVSEVDCPHFDHALAAYYLIL
PSEVSSNLARFDAMRYGLRVGDDGTRSAEEVMAMTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDA
AYRSVDVLVSPTTPTTAFRLGEKVDDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSPDDGLPVGLQIMAPALADDRLYRVG
AAYEAARGPLLSAI

Sequences:

>Translated_494_residues
MTDIIRSDAATLAAKIAIKEVSSTEITRACLDQIEATDETYHAFLHVAADEALAAAAAVDKQVAAGEPLPSALAGVPLAL
KDVFTTSDMPTTCGSKILEGWRSPYDATLTARLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNLDRVPGGSGGGS
AAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDS
TSVDAEVPDVVGAARAGAVGDLRGVRVGVVRQLHGGEGYQPGVLASFEAAVEQLTALGAEVSEVDCPHFDHALAAYYLIL
PSEVSSNLARFDAMRYGLRVGDDGTRSAEEVMAMTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDA
AYRSVDVLVSPTTPTTAFRLGEKVDDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSPDDGLPVGLQIMAPALADDRLYRVG
AAYEAARGPLLSAI
>Mature_493_residues
TDIIRSDAATLAAKIAIKEVSSTEITRACLDQIEATDETYHAFLHVAADEALAAAAAVDKQVAAGEPLPSALAGVPLALK
DVFTTSDMPTTCGSKILEGWRSPYDATLTARLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNLDRVPGGSGGGSA
AALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDST
SVDAEVPDVVGAARAGAVGDLRGVRVGVVRQLHGGEGYQPGVLASFEAAVEQLTALGAEVSEVDCPHFDHALAAYYLILP
SEVSSNLARFDAMRYGLRVGDDGTRSAEEVMAMTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAA
YRSVDVLVSPTTPTTAFRLGEKVDDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSPDDGLPVGLQIMAPALADDRLYRVGA
AYEAARGPLLSAI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=488, Percent_Identity=37.5, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI195972892, Length=496, Percent_Identity=24.1935483870968, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=425, Percent_Identity=37.4117647058824, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=25.9405940594059, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=146, Percent_Identity=36.986301369863, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=308, Percent_Identity=25.6493506493506, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=189, Percent_Identity=28.042328042328, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=416, Percent_Identity=32.6923076923077, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=447, Percent_Identity=28.8590604026846, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320448, Length=199, Percent_Identity=29.6482412060301, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=466, Percent_Identity=39.4849785407725, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24652985, Length=501, Percent_Identity=25.9481037924152, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922090, Length=501, Percent_Identity=25.9481037924152, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652981, Length=501, Percent_Identity=25.9481037924152, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652983, Length=501, Percent_Identity=25.9481037924152, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24644968, Length=524, Percent_Identity=25.1908396946565, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648435, Length=203, Percent_Identity=31.5270935960591, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648437, Length=203, Percent_Identity=31.5270935960591, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550774, Length=203, Percent_Identity=31.5270935960591, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21356731, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=25.0485436893204, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=134, Percent_Identity=35.0746268656716, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=134, Percent_Identity=35.0746268656716, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51437; Mature: 51305

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIIRSDAATLAAKIAIKEVSSTEITRACLDQIEATDETYHAFLHVAADEALAAAAAVD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KQVAAGEPLPSALAGVPLALKDVFTTSDMPTTCGSKILEGWRSPYDATLTARLRAAGIPI
HHHHCCCCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCE
LGKTNMDEFAMGSSTENSAYGPTRNPWNLDRVPGGSGGGSAAALAAFQAPLAIGSDTGGS
ECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCC
IRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDS
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
TSVDAEVPDVVGAARAGAVGDLRGVRVGVVRQLHGGEGYQPGVLASFEAAVEQLTALGAE
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
VSEVDCPHFDHALAAYYLILPSEVSSNLARFDAMRYGLRVGDDGTRSAEEVMAMTRAAGF
HHHCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCC
GPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAAYRSVDVLVSPTTPTTAFRL
CHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHH
GEKVDDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSPDDGLPVGLQIMAPALADDRLYRVG
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHEECC
AAYEAARGPLLSAI
CHHHHHCCCHHHCC
>Mature Secondary Structure 
TDIIRSDAATLAAKIAIKEVSSTEITRACLDQIEATDETYHAFLHVAADEALAAAAAVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KQVAAGEPLPSALAGVPLALKDVFTTSDMPTTCGSKILEGWRSPYDATLTARLRAAGIPI
HHHHCCCCCCHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCE
LGKTNMDEFAMGSSTENSAYGPTRNPWNLDRVPGGSGGGSAAALAAFQAPLAIGSDTGGS
ECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCC
IRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDS
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
TSVDAEVPDVVGAARAGAVGDLRGVRVGVVRQLHGGEGYQPGVLASFEAAVEQLTALGAE
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
VSEVDCPHFDHALAAYYLILPSEVSSNLARFDAMRYGLRVGDDGTRSAEEVMAMTRAAGF
HHHCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCC
GPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAAYRSVDVLVSPTTPTTAFRL
CHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHH
GEKVDDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSPDDGLPVGLQIMAPALADDRLYRVG
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHEECC
AAYEAARGPLLSAI
CHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]