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The map label for this gene is gltX [H]

Identifier: 121638873

GI number: 121638873

Start: 3303292

End: 3304764

Strand: Reverse

Name: gltX [H]

Synonym: BCG_3013c

Alternate gene names: 121638873

Gene position: 3304764-3303292 (Counterclockwise)

Preceding gene: 121638874

Following gene: 121638871

Centisome position: 75.55

GC content: 66.73

Gene sequence:

>1473_bases
GTGACCGCCACGGAAACAGTCCGGGTCCGATTCTGCCCTTCACCCACCGGAACTCCGCACGTCGGTCTGGTCCGCACCGC
GCTGTTCAACTGGGCCTACGCCCGGCACACCGGTGGCACCTTCGTGTTCCGCATCGAGGACACCGACGCCCAACGCGACA
GCGAGGAAAGCTATCTGGCGCTGCTGGACGCGCTGCGCTGGCTTGGCCTGGATTGGGATGAGGGGCCCGAGGTGGGTGGG
CCGTACGGGCCGTACCGGCAGTCGCAGCGCGCCGAAATCTACCGTGACGTGCTCGCCCGACTGCTCGCCGCGGGCGAGGC
CTACCACGCTTTCTCCACACCCGAGGAAGTGGAGGCCCGTCATGTCGCGGCCGGGCGCAATCCCAAGCTGGGTTACGACA
ATTTCGATCGTCACTTGACCGACGCGCAGCGTGCGGCGTACCTGGCGGAGGGCCGCCAGCCGGTGGTGCGGCTGCGGATG
CCCGATGACGACCTCGCTTGGAATGACCTGGTGCGCGGGCCCGTGACGTTCGCGGCTGGCTCCGTACCCGACTTCGCGTT
GACCCGAGCCAGCGGTGATCCGTTATACACCTTGGTCAACCCGTGCGACGACGCGCTGATGAAGATCACCCATGTGTTGC
GTGGCGAGGACCTGCTGCCGTCGACGCCGCGCCAGCTGGCGCTACATCAGGCGTTGATCAGGATCGGGGTAGCTGAGCGG
ATTCCGAAATTCGCGCACCTCCCAACGGTCTTGGGGGAGGGCACCAAGAAGCTCTCGAAGCGCGATCCGCAGTCGAACCT
GTTTGCCCACCGCGACCGCGGGTTCATCCCCGAAGGCCTGCTGAACTACCTCGCGTTGCTCGGCTGGTCCATAGCCGACG
ACCACGACCTGTTCGGTCTCGACGAGATGGTGGCCGCGTTCGACGTTGCCGACGTCAACTCCAGCCCGGCCCGGTTCGAC
CAGAAGAAGGCCGACGCGCTGAACGCCGAGCACATCCGGATGCTCGACGTGGGCGACTTCACGGTCAGGCTGCGCGACCA
TCTGGATACCCACGGCCATCACATCGCGTTGGATGAGGCGGCATTCGCCGCCGCCGCCGAGTTGGTGCAGACCCGCATCG
TGGTGCTCGGTGACGCGTGGGAGCTGTTGAAGTTCTTCAACGACGATCAGTATGTGATCGATCCCAAGGCCGCGGCCAAG
GAGCTAGGCCCAGACGGGGCCGCGGTGCTCGACGCGGCCCTGGCCGCGCTGACAAGTGCGACGGACTGGACGGCACCCCT
GATCGAGGCGGCCCTTAAGGACGCCTTGATCGAAGGTTTGGCGCTCAAACCCCGCAAGGCGTTCAGCCCCATCCGGGTGG
CCGCCACCGGCACGACCGTCAGCCCGCCGTTGTTTGAATCGCTGGAGCTGCTCGGTCGTGACCGCAGCATGCAGCGGCTG
CGCGCGGCGCGCCAGCTGGTCGGGCACGCGTGA

Upstream 100 bases:

>100_bases
CACCCGCGGGAGTCGGTCCCATCGAGGACGGCGACACCGTCTCGATCACCATCGAGGGAATCGGCACCCTCACCAATCCC
GTAGTCCGCAAAGGAAAGCC

Downstream 100 bases:

>100_bases
GCCTTGTGTGGCGAATCTTTGGTAGTCTGCACGTCGGCCCATAACAGCTGGCGGCAGCGGTTTTAGGCAGCCCATGGGGT
ATGGTGTAATTGGCAACACA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLALLDALRWLGLDWDEGPEVGG
PYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEARHVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRM
PDDDLAWNDLVRGPVTFAAGSVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGLDEMVAAFDVADVNSSPARFD
QKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEAAFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAK
ELGPDGAAVLDAALAALTSATDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
RAARQLVGHA

Sequences:

>Translated_490_residues
MTATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLALLDALRWLGLDWDEGPEVGG
PYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEARHVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRM
PDDDLAWNDLVRGPVTFAAGSVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGLDEMVAAFDVADVNSSPARFD
QKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEAAFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAK
ELGPDGAAVLDAALAALTSATDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
RAARQLVGHA
>Mature_489_residues
TATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLALLDALRWLGLDWDEGPEVGGP
YGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEARHVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRMP
DDDLAWNDLVRGPVTFAAGSVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAERI
PKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGLDEMVAAFDVADVNSSPARFDQ
KKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEAAFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAKE
LGPDGAAVLDAALAALTSATDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRLR
AARQLVGHA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=33.0645161290323, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1788743, Length=488, Percent_Identity=34.4262295081967, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI226510925, Length=265, Percent_Identity=29.811320754717, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=29.4949494949495, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=30.4950495049505, Blast_Score=208, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24665321, Length=496, Percent_Identity=32.0564516129032, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53836; Mature: 53705

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLA
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
LLDALRWLGLDWDEGPEVGGPYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEAR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
HVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRMPDDDLAWNDLVRGPVTFAAG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHCCC
SVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
CCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGL
CCHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
DEMVAAFDVADVNSSPARFDQKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHCCCCCCEEEEHHH
AFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAKELGPDGAAVLDAALAALTSA
HHHHHHHHHHHHEEEECCHHHHHHHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHCC
TDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
CCCHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
RAARQLVGHA
HHHHHHHCCC
>Mature Secondary Structure 
TATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLA
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
LLDALRWLGLDWDEGPEVGGPYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEAR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
HVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRMPDDDLAWNDLVRGPVTFAAG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHCCC
SVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
CCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGL
CCHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
DEMVAAFDVADVNSSPARFDQKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHCCCCCCEEEEHHH
AFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAKELGPDGAAVLDAALAALTSA
HHHHHHHHHHHHEEEECCHHHHHHHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHCC
TDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
CCCHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
RAARQLVGHA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]