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The map label for this gene is istB [H]

Identifier: 121638826

GI number: 121638826

Start: 3243694

End: 3244224

Strand: Direct

Name: istB [H]

Synonym: BCG_2966

Alternate gene names: 121638826

Gene position: 3243694-3244224 (Clockwise)

Preceding gene: 121638825

Following gene: 121638833

Centisome position: 74.15

GC content: 64.97

Gene sequence:

>531_bases
ATGCCCACCACCAAAGCCACCCAGCGCCGTGATGTTTCCACCGAGATCGCTTACCTGACAAGAGCATTGAAAGCTCCCAC
CCTGCGTGAGTCAGTGTCCCGGCTGGCCGATCGCGCCCGCGCCGAGAACTGGAGCCACGAAGAATACCTGGCCGCCTGCC
TGCAGCGGGAAGTGTCAGCCCGGGAGTCCCATGGTGGTGAGGGCCGCATCCGCGCCGCCCGCTTCCCGGCTCGGAAGTCG
TTGGAAGAGTTCGACTTTGAGCATGCTCGTGGCCTCAAACGCGACACCATCGCACATCTGGGCACCCTGGATTTCATCAC
CGCCCGCGATAACGTCGTGTTTTTGGGCCCCGCCTGGCACCGGGAAGACTCATCTTGCGGTCGGCCTGGCGATACGCGCG
TGTCAGGCCGGTCATCGGGTGCTGTTCGCCACCGCCGCCGAATGGGTAGCACGGCTCGCCGAGGCTCACCACGCCGGGCG
CATCTACGCCGAACTCACCCGGCTTTGCCGCTATCCGCTCCTGGTGGTTGA

Upstream 100 bases:

>100_bases
TCAGTGCAGCATCACCGGTAGTTGAGCCGCAGGTGCAGGTCCGCTCACTGAGCGACTACGATGACGCGCTGGGAGTCGAC
ATCGATGGCGGGGTGGCCTG

Downstream 100 bases:

>100_bases
CGAAGTCGGCTACATTCCGTTTGAGCCCGAGGCCGCCAACCTCTTCTTCCAGCTGGTGTCCTCCCGGTATGAGCGGGCCA
GCTTGATCGTCACGTCCAAT

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPTTKATQRRDVSTEIAYLTRALKAPTLRESVSRLADRARAENWSHEEYLAACLQREVSARESHGGEGRIRAARFPARKS
LEEFDFEHARGLKRDTIAHLGTLDFITARDNVVFLGPAWHREDSSCGRPGDTRVSGRSSGAVRHRRRMGSTARRGSPRRA
HLRRTHPALPLSAPGG

Sequences:

>Translated_176_residues
MPTTKATQRRDVSTEIAYLTRALKAPTLRESVSRLADRARAENWSHEEYLAACLQREVSARESHGGEGRIRAARFPARKS
LEEFDFEHARGLKRDTIAHLGTLDFITARDNVVFLGPAWHREDSSCGRPGDTRVSGRSSGAVRHRRRMGSTARRGSPRRA
HLRRTHPALPLSAPGG
>Mature_175_residues
PTTKATQRRDVSTEIAYLTRALKAPTLRESVSRLADRARAENWSHEEYLAACLQREVSARESHGGEGRIRAARFPARKSL
EEFDFEHARGLKRDTIAHLGTLDFITARDNVVFLGPAWHREDSSCGRPGDTRVSGRSSGAVRHRRRMGSTARRGSPRRAH
LRRTHPALPLSAPGG

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 19658; Mature: 19527

Theoretical pI: Translated: 11.84; Mature: 11.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTKATQRRDVSTEIAYLTRALKAPTLRESVSRLADRARAENWSHEEYLAACLQREVSA
CCCCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RESHGGEGRIRAARFPARKSLEEFDFEHARGLKRDTIAHLGTLDFITARDNVVFLGPAWH
HHCCCCCCCEEECCCCHHHHHHHHCHHHHCCCCHHHHHHHCCHHEEECCCCEEEECCCCC
REDSSCGRPGDTRVSGRSSGAVRHRRRMGSTARRGSPRRAHLRRTHPALPLSAPGG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PTTKATQRRDVSTEIAYLTRALKAPTLRESVSRLADRARAENWSHEEYLAACLQREVSA
CCCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RESHGGEGRIRAARFPARKSLEEFDFEHARGLKRDTIAHLGTLDFITARDNVVFLGPAWH
HHCCCCCCCEEECCCCHHHHHHHHCHHHHCCCCHHHHHHHCCHHEEECCCCEEEECCCCC
REDSSCGRPGDTRVSGRSSGAVRHRRRMGSTARRGSPRRAHLRRTHPALPLSAPGG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]