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The map label for this gene is clpP2

Identifier: 121638343

GI number: 121638343

Start: 2727701

End: 2728345

Strand: Reverse

Name: clpP2

Synonym: BCG_2480c

Alternate gene names: 121638343

Gene position: 2728345-2727701 (Counterclockwise)

Preceding gene: 121638344

Following gene: 121638340

Centisome position: 62.37

GC content: 61.71

Gene sequence:

>645_bases
GTGAATTCCCAAAATTCTCAGATCCAGCCCCAGGCGCGCTACATCCTGCCGTCGTTCATCGAGCACTCCAGCTTCGGGGT
CAAGGAGTCCAATCCATACAACAAGCTGTTCGAGGAACGCATCATCTTCCTCGGCGTCCAGGTCGACGACGCGTCGGCGA
ACGACATCATGGCACAGTTGCTGGTGTTGGAGTCGTTGGATCCCGACCGCGATATCACCATGTACATCAACTCGCCGGGC
GGTGGGTTCACCTCGCTGATGGCGATCTACGACACCATGCAATACGTGCGGGCCGATATCCAGACGGTGTGTCTGGGCCA
GGCCGCCTCGGCGGCTGCGGTGCTGCTGGCCGCCGGAACACCGGGCAAGCGCATGGCGCTGCCGAATGCGCGGGTGTTGA
TCCATCAGCCGTCGTTGTCGGGCGTGATCCAGGGACAGTTCTCCGATCTGGAGATCCAGGCCGCCGAGATCGAGCGGATG
CGCACCCTGATGGAAACCACGCTGGCCCGCCACACCGGCAAGGACGCCGGAGTGATCCGCAAAGACACTGACCGGGACAA
GATCTTGACCGCGGAAGAGGCTAAGGACTACGGCATCATCGACACGGTGCTCGAGTACCGGAAGCTCTCCGCGCAAACCG
CCTGA

Upstream 100 bases:

>100_bases
CGGATTCCGATCGCGACCGCTGGTTCACCGCCGCCGAAGCCCTGGAATACGGTTTCGTCGATCACATCATCACCCGCGCC
CACGTCAATGGAGAAGCACA

Downstream 100 bases:

>100_bases
GCCATGGGCGGGTGGTGCGGCCGTGGCGTGGCTTGGTGGTCGCCACCCGACATGCGCCGAGATCCACGCCAGAGCGGAAA
ATCACCATAAGGCGTGACGC

Product: ATP-dependent Clp protease proteolytic subunit

Products: NA

Alternate protein names: Endopeptidase Clp 2

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNSQNSQIQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIFLGVQVDDASANDIMAQLLVLESLDPDRDITMYINSPG
GGFTSLMAIYDTMQYVRADIQTVCLGQAASAAAVLLAAGTPGKRMALPNARVLIHQPSLSGVIQGQFSDLEIQAAEIERM
RTLMETTLARHTGKDAGVIRKDTDRDKILTAEEAKDYGIIDTVLEYRKLSAQTA

Sequences:

>Translated_214_residues
MNSQNSQIQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIFLGVQVDDASANDIMAQLLVLESLDPDRDITMYINSPG
GGFTSLMAIYDTMQYVRADIQTVCLGQAASAAAVLLAAGTPGKRMALPNARVLIHQPSLSGVIQGQFSDLEIQAAEIERM
RTLMETTLARHTGKDAGVIRKDTDRDKILTAEEAKDYGIIDTVLEYRKLSAQTA
>Mature_214_residues
MNSQNSQIQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIFLGVQVDDASANDIMAQLLVLESLDPDRDITMYINSPG
GGFTSLMAIYDTMQYVRADIQTVCLGQAASAAAVLLAAGTPGKRMALPNARVLIHQPSLSGVIQGQFSDLEIQAAEIERM
RTLMETTLARHTGKDAGVIRKDTDRDKILTAEEAKDYGIIDTVLEYRKLSAQTA

Specific function: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family

Homologues:

Organism=Homo sapiens, GI5174419, Length=190, Percent_Identity=44.2105263157895, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1786641, Length=194, Percent_Identity=48.9690721649485, Blast_Score=200, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17538017, Length=193, Percent_Identity=41.4507772020725, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI20129427, Length=192, Percent_Identity=43.2291666666667, Blast_Score=161, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLPP2_MYCBO (P63784)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856134.1
- ProteinModelPortal:   P63784
- SMR:   P63784
- MEROPS:   S14.009
- EnsemblBacteria:   EBMYCT00000015258
- GeneID:   1094227
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2487c
- GeneTree:   EBGT00050000015874
- HOGENOM:   HBG558421
- OMA:   QDPYTKL
- ProtClustDB:   PRK12553
- BioCyc:   MBOV233413:MB2487C-MONOMER
- BRENDA:   3.4.21.92
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00444
- InterPro:   IPR001907
- InterPro:   IPR018215
- PANTHER:   PTHR10381
- PRINTS:   PR00127

Pfam domain/function: PF00574 CLP_protease

EC number: =3.4.21.92

Molecular weight: Translated: 23540; Mature: 23540

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00382 CLP_PROTEASE_HIS; PS00381 CLP_PROTEASE_SER

Important sites: ACT_SITE 110-110 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQNSQIQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIFLGVQVDDASANDIMAQL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEEEEEEECCCCHHHHHHHH
LVLESLDPDRDITMYINSPGGGFTSLMAIYDTMQYVRADIQTVCLGQAASAAAVLLAAGT
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
PGKRMALPNARVLIHQPSLSGVIQGQFSDLEIQAAEIERMRTLMETTLARHTGKDAGVIR
CCCEEECCCCEEEEECCCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEE
KDTDRDKILTAEEAKDYGIIDTVLEYRKLSAQTA
CCCCCCCEEEHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSQNSQIQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIFLGVQVDDASANDIMAQL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEEEEEEECCCCHHHHHHHH
LVLESLDPDRDITMYINSPGGGFTSLMAIYDTMQYVRADIQTVCLGQAASAAAVLLAAGT
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
PGKRMALPNARVLIHQPSLSGVIQGQFSDLEIQAAEIERMRTLMETTLARHTGKDAGVIR
CCCEEECCCCEEEEECCCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEE
KDTDRDKILTAEEAKDYGIIDTVLEYRKLSAQTA
CCCCCCCEEEHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972