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The map label for this gene is cysH

Identifier: 121638271

GI number: 121638271

Start: 2651389

End: 2652153

Strand: Direct

Name: cysH

Synonym: BCG_2406

Alternate gene names: 121638271

Gene position: 2651389-2652153 (Clockwise)

Preceding gene: 121638270

Following gene: 121638272

Centisome position: 60.61

GC content: 64.18

Gene sequence:

>765_bases
ATGAGCGGCGAGACAACCAGGCTGACCGAACCGCAACTACGTGAGCTGGCCGCGCGCGGAGCTGCCGAACTCGACGGCGC
CACCGCCACCGACATGTTGCGCTGGACCGACGAAACCTTCGGCGACATCGGCGGCGCCGGCGGCGGCGTGAGCGGACATC
GCGGGTGGACAACGTGCAACTACGTAGTTGCTTCCAACATGGCTGATGCGGTGCTGGTGGATCTGGCCGCCAAGGTGCGA
CCGGGCGTACCGGTCATCTTTCTTGATACCGGCTACCACTTCGTCGAAACAATCGGCACCAGAGATGCGATCGAGTCCGT
CTATGACGTCCGGGTGCTCAATGTCACTCCGGAGCACACAGTGGCCGAGCAGGACGAACTGCTGGGCAAGGACTTGTTCG
CCCGCAACCCCCATGAATGCTGCCGGTTGCGCAAGGTCGTTCCCCTGGGCAAGACGCTGCGTGGCTACTCCGCGTGGGTG
ACCGGGCTACGGCGGGTCGATGCACCGACCCGGGCCAATGCCCCGCTGGTCAGCTTCGATGAGACGTTCAAACTAGTGAA
GGTCAACCCGCTGGCGGCGTGGACCGACCAAGATGTGCAGGAATACATTGCCGACAACGACGTGCTGGTTAATCCGCTTG
TGCGGGAAGGCTATCCGTCGATCGGTTGCGCTCCGTGCACAGCCAAACCCGCCGAAGGCGCCGACCCGCGCAGCGGACGC
TGGCAGGGGCTGGCCAAGACCGAATGCGGGTTGCACGCCTCGTGA

Upstream 100 bases:

>100_bases
GCGACTACATCGACCGGGTGGTGCGCAACTTCGTCAAACACCGCAGCGAAGGTGAACGCTTCGCGCAGTGGGTCATCCGG
GCCGAGGAGGACGACCTGCG

Downstream 100 bases:

>100_bases
CCGCGCCGGCGACGATGCAGAGCGCAGCGATGCTGAGGAGCGGCGCCATCGAAGCACCGCCGGCGACGATGCAGAGCGCA
GCGATGCTGAGGAGCGGCGC

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSGETTRLTEPQLRELAARGAAELDGATATDMLRWTDETFGDIGGAGGGVSGHRGWTTCNYVVASNMADAVLVDLAAKVR
PGVPVIFLDTGYHFVETIGTRDAIESVYDVRVLNVTPEHTVAEQDELLGKDLFARNPHECCRLRKVVPLGKTLRGYSAWV
TGLRRVDAPTRANAPLVSFDETFKLVKVNPLAAWTDQDVQEYIADNDVLVNPLVREGYPSIGCAPCTAKPAEGADPRSGR
WQGLAKTECGLHAS

Sequences:

>Translated_254_residues
MSGETTRLTEPQLRELAARGAAELDGATATDMLRWTDETFGDIGGAGGGVSGHRGWTTCNYVVASNMADAVLVDLAAKVR
PGVPVIFLDTGYHFVETIGTRDAIESVYDVRVLNVTPEHTVAEQDELLGKDLFARNPHECCRLRKVVPLGKTLRGYSAWV
TGLRRVDAPTRANAPLVSFDETFKLVKVNPLAAWTDQDVQEYIADNDVLVNPLVREGYPSIGCAPCTAKPAEGADPRSGR
WQGLAKTECGLHAS
>Mature_253_residues
SGETTRLTEPQLRELAARGAAELDGATATDMLRWTDETFGDIGGAGGGVSGHRGWTTCNYVVASNMADAVLVDLAAKVRP
GVPVIFLDTGYHFVETIGTRDAIESVYDVRVLNVTPEHTVAEQDELLGKDLFARNPHECCRLRKVVPLGKTLRGYSAWVT
GLRRVDAPTRANAPLVSFDETFKLVKVNPLAAWTDQDVQEYIADNDVLVNPLVREGYPSIGCAPCTAKPAEGADPRSGRW
QGLAKTECGLHAS

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=212, Percent_Identity=30.188679245283, Blast_Score=87, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6325425, Length=195, Percent_Identity=32.3076923076923, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_MYCBO (P65669)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856062.1
- ProteinModelPortal:   P65669
- SMR:   P65669
- EnsemblBacteria:   EBMYCT00000014314
- GeneID:   1094304
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2413
- GeneTree:   EBGT00050000017137
- HOGENOM:   HBG758022
- OMA:   FEETLAY
- ProtClustDB:   PRK02090
- BioCyc:   MBOV233413:MB2413-MONOMER
- BRENDA:   1.8.4.8
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR02055
- TIGRFAMs:   TIGR00434

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 27423; Mature: 27292

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGETTRLTEPQLRELAARGAAELDGATATDMLRWTDETFGDIGGAGGGVSGHRGWTTCN
CCCCCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEH
YVVASNMADAVLVDLAAKVRPGVPVIFLDTGYHFVETIGTRDAIESVYDVRVLNVTPEHT
HHHHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCHHHHHHHHCEEEEEECCCCH
VAEQDELLGKDLFARNPHECCRLRKVVPLGKTLRGYSAWVTGLRRVDAPTRANAPLVSFD
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
ETFKLVKVNPLAAWTDQDVQEYIADNDVLVNPLVREGYPSIGCAPCTAKPAEGADPRSGR
CCEEEEEECCCCCCCHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
WQGLAKTECGLHAS
CCCCCCCCCCCCCC
>Mature Secondary Structure 
SGETTRLTEPQLRELAARGAAELDGATATDMLRWTDETFGDIGGAGGGVSGHRGWTTCN
CCCCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEH
YVVASNMADAVLVDLAAKVRPGVPVIFLDTGYHFVETIGTRDAIESVYDVRVLNVTPEHT
HHHHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCHHHHHHHHCEEEEEECCCCH
VAEQDELLGKDLFARNPHECCRLRKVVPLGKTLRGYSAWVTGLRRVDAPTRANAPLVSFD
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
ETFKLVKVNPLAAWTDQDVQEYIADNDVLVNPLVREGYPSIGCAPCTAKPAEGADPRSGR
CCEEEEEECCCCCCCHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
WQGLAKTECGLHAS
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972