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The map label for this gene is mbtJ [H]

Identifier: 121638264

GI number: 121638264

Start: 2642751

End: 2643671

Strand: Direct

Name: mbtJ [H]

Synonym: BCG_2399

Alternate gene names: 121638264

Gene position: 2642751-2643671 (Clockwise)

Preceding gene: 121638263

Following gene: 121638266

Centisome position: 60.41

GC content: 66.23

Gene sequence:

>921_bases
ATGGTGTTGCGGCCCATCACCGGGGCGATTCCGCCAGACGGGCCGTGGGGGATATGGGCCTCGCGCCGGATCATCGCCGG
ACTCATGGGCACGTTCGGGCCCTCGCTCGCGGGCACCCGAGTGGAACAAGTCAACTCCGTTCTGCCGGACGGACGCCGGG
TCGTCGGCGAATGGGTGTATGGACCGCACAACAACGCGATCAATGCCGGACCCGGTGGCGGCGCCATCTATTACGTACAC
GGCAGCGGTTACACGATGTGTTCGCCCCGAACCCACCGGCGGCTGACATCCTGGCTGTCGTCATTGACCGGGCTACCGGT
ATTCAGTGTCGATTACCGACTGGCGCCGCGCTACCGTTTCCCGACCGCGGCCACCGACGTGCGGGCAGCCTGGGATTGGT
TAGCGCACGTATGCGGCTTAGCCGCGGAGCACATGGTGATCGCCGCGGATTCCGCGGGTGGCCATCTGACCGTCGACATG
CTGCTGCAACCCGAGGTCGCCGCCCGACCTCCGGCGGCGGTGGTGTTGTTTTCGCCGCTGATCGACCTCACCTTCCGGCT
GGGCGCCAGTCGTGAGCTGCAGCGCCCCGATCCTGTCGTGCGCGCTGACCGTGCGGCCCGGTCGGTTGCGCTGTACTACA
CCGGAGTCGATCCCGCCCACCACCGGCTGGCGCTCGATGTTGCCGGCGGGCCACCGCTGCCACCGACGCTGATCCAGGTG
GGTGGAGCCGAGATACTCGAGGCCGATGCGAGACAACTCGATGCCGACATCCGCGCTGCCGGCGGCATATGCGAGTTGCA
AGTGTGGCCTGATCAGATGCATGTGTTCCAGGCCCTGCCGCGGATGACGCCCGAAGCGGCCAAAGCCATGACCTATGTTG
CCCAGTTCATCCGCAGTACAACAGCACGTGGAGACCTCTGA

Upstream 100 bases:

>100_bases
ATTGACTGACGTCAACAAGTTGAATTGACTGCGTTGCATGACCGACGGTGTTCCGGCCCGCGGGTCACTTCGATCACGCG
GCGCGGTAGCGGTGAGCTCG

Downstream 100 bases:

>100_bases
ACGTTACTGGCGTGCAACCAGATAAGGCGTCAATGTGGATAGCTTTTCGCAAGTCTCCTCGAATTCGCGCTCTGGCTCCG
ATTCTTCGATGATGCCGGCG

Product: putative acetyl hydrolase mbtJ

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIYYVH
GSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDM
LLQPEVAARPPAAVVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRSTTARGDL

Sequences:

>Translated_306_residues
MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIYYVH
GSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDM
LLQPEVAARPPAAVVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRSTTARGDL
>Mature_306_residues
MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVYGPHNNAINAGPGGGAIYYVH
GSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRFPTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDM
LLQPEVAARPPAAVVLFSPLIDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRSTTARGDL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533410, Length=148, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=148, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 32877; Mature: 32877

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY
CEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEHHHEEE
GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF
CCCCCCCCCCCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHCCCCEEEECEEECCCCCC
PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL
CCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEHHHH
IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
HHHHHHCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHC
GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST
CCHHHHHHHHHHHCHHHHHCCCEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TARGDL
CCCCCC
>Mature Secondary Structure
MVLRPITGAIPPDGPWGIWASRRIIAGLMGTFGPSLAGTRVEQVNSVLPDGRRVVGEWVY
CEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEHHHEEE
GPHNNAINAGPGGGAIYYVHGSGYTMCSPRTHRRLTSWLSSLTGLPVFSVDYRLAPRYRF
CCCCCCCCCCCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHCCCCEEEECEEECCCCCC
PTAATDVRAAWDWLAHVCGLAAEHMVIAADSAGGHLTVDMLLQPEVAARPPAAVVLFSPL
CCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEHHHH
IDLTFRLGASRELQRPDPVVRADRAARSVALYYTGVDPAHHRLALDVAGGPPLPPTLIQV
HHHHHHCCCCCCCCCCCCHHHHHHHHCEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHC
GGAEILEADARQLDADIRAAGGICELQVWPDQMHVFQALPRMTPEAAKAMTYVAQFIRST
CCHHHHHHHHHHHCHHHHHCCCEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TARGDL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]