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The map label for this gene is rsmE

Identifier: 121638251

GI number: 121638251

Start: 2617059

End: 2617847

Strand: Reverse

Name: rsmE

Synonym: BCG_2386c

Alternate gene names: 121638251

Gene position: 2617847-2617059 (Counterclockwise)

Preceding gene: 121638252

Following gene: 121638249

Centisome position: 59.84

GC content: 69.46

Gene sequence:

>789_bases
ATGGTGGCGATGCTGTTTTACGTCGACACACTGCCCGACACCGGTGCGGTGGCGGTCGTAGACGGCGACGAAGGATTTCA
CGCCGCCACCGTGCGGCGGATCCGTCCCGGCGAGCAGTTGGTGCTTGGTGACGGTGTCGGTCGCCTGGCCCGCTGTGTGG
TGGAGCAGGCCGGACGTGGCGGGCTGCGGGCCCGGGTGCTTAGGCGCTGGAGCGTCCCGCCCGTGCGGCCACCGGTGACC
GTGGTGCAGGCGCTGCCCAAATCCGAACGCTCCGAATTGGCAATCGAGCTGGCCACCGAAGCCGGAGCCGACGCGTTCCT
GGCATGGCAGGCGGCGCGCTGCGTGGCGAACTGGGACGGGGCGCGTGTCGACAAGGGTTTGCGTCGGTGGCGAGCGGTGG
TCCGCTCGGCGGCCCGGCAATCTCGTCGGGCGCGCATCCCGCCGGTCGACGGCGTGTTGTCGACGCCAATGCTTGTACAG
CGTGTCCGAGAAGAGGTGGCCGCCGGCGCGGCGGTGCTGGTCCTGCACGAGGAGGCGACCGAGCGGATTGTGGATATCGC
TGCGGCGCAAGCTGGTTCGTTGATGCTCGTGGTTGGACCCGAGGGTGGGATCGCACCGGACGAGCTTGCCGCGCTGACCG
ATGCCGGCGCCGTCGCGGTCCGGCTGGGCCCGACGGTGCTGCGGACATCGACCGCGGCCGCGGTGGCATTGGGTGCTGTC
GGTGTGCTCACATCTCGTTGGGATGCATCAGCGAGCGACTGTGAATACTGCGACGTGACACGCCGGTAG

Upstream 100 bases:

>100_bases
GACCGCGAGGTGGCCGAGGTCCGTTCGACCCACGCCGCCGCCGGCGGACTGTTCAGCCGGTTGCGCGAGACCTTCACCGG
GCGCTAATCACGGCCCGCAC

Downstream 100 bases:

>100_bases
CCGGTCGTCAGAATCCCACTCGCCGGCTTGGCCGGGCGCACCCGAGCCGCCGGCCCCGCCGTTGCCCGGTCTCGGGCGCC
CCAGCGGCCCCATCACCATC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MVAMLFYVDTLPDTGAVAVVDGDEGFHAATVRRIRPGEQLVLGDGVGRLARCVVEQAGRGGLRARVLRRWSVPPVRPPVT
VVQALPKSERSELAIELATEAGADAFLAWQAARCVANWDGARVDKGLRRWRAVVRSAARQSRRARIPPVDGVLSTPMLVQ
RVREEVAAGAAVLVLHEEATERIVDIAAAQAGSLMLVVGPEGGIAPDELAALTDAGAVAVRLGPTVLRTSTAAAVALGAV
GVLTSRWDASASDCEYCDVTRR

Sequences:

>Translated_262_residues
MVAMLFYVDTLPDTGAVAVVDGDEGFHAATVRRIRPGEQLVLGDGVGRLARCVVEQAGRGGLRARVLRRWSVPPVRPPVT
VVQALPKSERSELAIELATEAGADAFLAWQAARCVANWDGARVDKGLRRWRAVVRSAARQSRRARIPPVDGVLSTPMLVQ
RVREEVAAGAAVLVLHEEATERIVDIAAAQAGSLMLVVGPEGGIAPDELAALTDAGAVAVRLGPTVLRTSTAAAVALGAV
GVLTSRWDASASDCEYCDVTRR
>Mature_262_residues
MVAMLFYVDTLPDTGAVAVVDGDEGFHAATVRRIRPGEQLVLGDGVGRLARCVVEQAGRGGLRARVLRRWSVPPVRPPVT
VVQALPKSERSELAIELATEAGADAFLAWQAARCVANWDGARVDKGLRRWRAVVRSAARQSRRARIPPVDGVLSTPMLVQ
RVREEVAAGAAVLVLHEEATERIVDIAAAQAGSLMLVVGPEGGIAPDELAALTDAGAVAVRLGPTVLRTSTAAAVALGAV
GVLTSRWDASASDCEYCDVTRR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family

Homologues:

Organism=Escherichia coli, GI87082186, Length=240, Percent_Identity=25.4166666666667, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSME_MYCBO (P67203)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856042.1
- ProteinModelPortal:   P67203
- SMR:   P67203
- EnsemblBacteria:   EBMYCT00000017984
- GeneID:   1094381
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2393c
- GeneTree:   EBGT00050000017325
- HOGENOM:   HBG664092
- OMA:   QCERSIL
- ProtClustDB:   PRK11713
- BioCyc:   MBOV233413:MB2393C-MONOMER
- GO:   GO:0005737
- InterPro:   IPR015947
- InterPro:   IPR006700
- TIGRFAMs:   TIGR00046

Pfam domain/function: PF04452 Methyltrans_RNA; SSF88697 PUA-like

EC number: =2.1.1.193

Molecular weight: Translated: 27653; Mature: 27653

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAMLFYVDTLPDTGAVAVVDGDEGFHAATVRRIRPGEQLVLGDGVGRLARCVVEQAGRG
CEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCC
GLRARVLRRWSVPPVRPPVTVVQALPKSERSELAIELATEAGADAFLAWQAARCVANWDG
CHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHCCCC
ARVDKGLRRWRAVVRSAARQSRRARIPPVDGVLSTPMLVQRVREEVAAGAAVLVLHEEAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHCCEEEEEEEHHHH
ERIVDIAAAQAGSLMLVVGPEGGIAPDELAALTDAGAVAVRLGPTVLRTSTAAAVALGAV
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHH
GVLTSRWDASASDCEYCDVTRR
HHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVAMLFYVDTLPDTGAVAVVDGDEGFHAATVRRIRPGEQLVLGDGVGRLARCVVEQAGRG
CEEEEEEEECCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCC
GLRARVLRRWSVPPVRPPVTVVQALPKSERSELAIELATEAGADAFLAWQAARCVANWDG
CHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHCCCC
ARVDKGLRRWRAVVRSAARQSRRARIPPVDGVLSTPMLVQRVREEVAAGAAVLVLHEEAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHCCEEEEEEEHHHH
ERIVDIAAAQAGSLMLVVGPEGGIAPDELAALTDAGAVAVRLGPTVLRTSTAAAVALGAV
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHH
GVLTSRWDASASDCEYCDVTRR
HHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972