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The map label for this gene is rfe [H]

Identifier: 121637231

GI number: 121637231

Start: 1484822

End: 1486036

Strand: Direct

Name: rfe [H]

Synonym: BCG_1362

Alternate gene names: 121637231

Gene position: 1484822-1486036 (Clockwise)

Preceding gene: 121637230

Following gene: 121637232

Centisome position: 33.94

GC content: 65.19

Gene sequence:

>1215_bases
GTGCAGTACGGTCTCGAGGTGTCCAGCGATGTGGCCGGCGTTGCCGGTGGCTTGCTCGCCCTGTCCTATCGCGGCGCCGG
TGTCCCGCTGCGTGAGCTTGCGCTGGTCGGGCTGACCGCGGCGATCATCACCTATTTTGCGACCGGTCCGGTGCGGATGC
TGGCCAGTCGCCTGGGAGCCGTCGCCTACCCGCGGGAGCGAGATGTGCACGTCACGCCTACCCCTCGGATGGGTGGGTTG
GCGATGTTCCTGGGCATTGTCGGCGCCGTCTTTCTTGCCTCCCAGCTTCCGGCACTCACCCGGGGGTTCGTCTATTCCAC
CGGCATGCCCGCGGTGCTGGTGGCCGGTGCGGTGATCATGGGCATCGGCCTGATCGATGATCGTTGGGGTCTGGATGCAC
TGACGAAGTTCGCCGGCCAGATCACGGCGGCGAGCGTTCTGGTCACCATGGGTGTCGCCTGGAGTGTCCTGTACATCCCG
GTGGGTGGTGTGGGCACCATCGTCTTGGACCAGGCTTCCTCGATCCTGCTTACCCTGGCGCTGACCGTTTCGATCGTCAA
CGCGATGAACTTTGTCGACGGTCTCGACGGGCTGGCCGCCGGCCTGGGCCTGATAACGGCGCTGGCAATCTGCATGTTCT
CGGTGGGTTTGCTTCGTGACCACGGTGGTGACGTTTTGTACTACCCGCCGGCGGTGATTTCGGTGGTCCTGGCCGGGGCC
TGCCTGGGCTTTCTGCCACACAACTTCCACCGGGCCAAGATCTTCATGGGCGATTCCGGGTCGATGCTGATCGGCCTGAT
GCTGGCCGCCGCTTCCACCACCGCGGCCGGGCCGATCTCGCAGAACGCCTACGGCGCTCGTGATGTATTTGCTTTGCTGT
CGCCGTTCCTGCTGGTGGTGGCGGTCATGTTTGTGCCAATGCTCGACCTGCTGCTAGCGATCGTCCGTCGCACCCGCGCC
GGCCGCAGCGCGTTTAGCCCGGACAAAATGCACCTGCATCACCGGCTGCTGCAGATCGGTCATTCCCATCGGCGCGTGGT
CCTGATCATCTACCTGTGGGTGGGCATCGGTGCCTTCGGCGCCGCGAGCTCGATCTTCTTTAACCCGCGCGACACCGCGG
CGGTGATGCTGGGCGCGATCGTGGTCGCCGGCGTCGCGACACTGATCCCCCTGTTGCGCCGCGGCGACGACTACTACGAC
CCGGACCTGGACTAG

Upstream 100 bases:

>100_bases
CCAGCTTGTTCGGCTAGCCGCCGAACGTGCACGCACTGCGAAGATTCGGCCAATTGTTCGCAGCTGTTGCACGTTCGGCG
AGTGTTCAGCTCTCAGGTTG

Downstream 100 bases:

>100_bases
CCCGGAGCCGAGAACTACGACAAGGAGTAGTAGTGGTGTCTACCTTGTGGTACGGTGCGGCTAGAACCCCGAAGGAGACC
TCGCGGGTTGCCGGCCCCCG

Product: putative undecapaprenyl-phosphate alpha-n-acetylglucosaminyltransferase rfe

Products: UMP; Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-Phosphate GlcNAc-1-phosphate transferase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQYGLEVSSDVAGVAGGLLALSYRGAGVPLRELALVGLTAAIITYFATGPVRMLASRLGAVAYPRERDVHVTPTPRMGGL
AMFLGIVGAVFLASQLPALTRGFVYSTGMPAVLVAGAVIMGIGLIDDRWGLDALTKFAGQITAASVLVTMGVAWSVLYIP
VGGVGTIVLDQASSILLTLALTVSIVNAMNFVDGLDGLAAGLGLITALAICMFSVGLLRDHGGDVLYYPPAVISVVLAGA
CLGFLPHNFHRAKIFMGDSGSMLIGLMLAAASTTAAGPISQNAYGARDVFALLSPFLLVVAVMFVPMLDLLLAIVRRTRA
GRSAFSPDKMHLHHRLLQIGHSHRRVVLIIYLWVGIGAFGAASSIFFNPRDTAAVMLGAIVVAGVATLIPLLRRGDDYYD
PDLD

Sequences:

>Translated_404_residues
MQYGLEVSSDVAGVAGGLLALSYRGAGVPLRELALVGLTAAIITYFATGPVRMLASRLGAVAYPRERDVHVTPTPRMGGL
AMFLGIVGAVFLASQLPALTRGFVYSTGMPAVLVAGAVIMGIGLIDDRWGLDALTKFAGQITAASVLVTMGVAWSVLYIP
VGGVGTIVLDQASSILLTLALTVSIVNAMNFVDGLDGLAAGLGLITALAICMFSVGLLRDHGGDVLYYPPAVISVVLAGA
CLGFLPHNFHRAKIFMGDSGSMLIGLMLAAASTTAAGPISQNAYGARDVFALLSPFLLVVAVMFVPMLDLLLAIVRRTRA
GRSAFSPDKMHLHHRLLQIGHSHRRVVLIIYLWVGIGAFGAASSIFFNPRDTAAVMLGAIVVAGVATLIPLLRRGDDYYD
PDLD
>Mature_404_residues
MQYGLEVSSDVAGVAGGLLALSYRGAGVPLRELALVGLTAAIITYFATGPVRMLASRLGAVAYPRERDVHVTPTPRMGGL
AMFLGIVGAVFLASQLPALTRGFVYSTGMPAVLVAGAVIMGIGLIDDRWGLDALTKFAGQITAASVLVTMGVAWSVLYIP
VGGVGTIVLDQASSILLTLALTVSIVNAMNFVDGLDGLAAGLGLITALAICMFSVGLLRDHGGDVLYYPPAVISVVLAGA
CLGFLPHNFHRAKIFMGDSGSMLIGLMLAAASTTAAGPISQNAYGARDVFALLSPFLLVVAVMFVPMLDLLLAIVRRTRA
GRSAFSPDKMHLHHRLLQIGHSHRRVVLIIYLWVGIGAFGAASSIFFNPRDTAAVMLGAIVVAGVATLIPLLRRGDDYYD
PDLD

Specific function: Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP- C55), the lipid intermediate involved in the synthesis of various bacter

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family. WecA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790218, Length=313, Percent_Identity=29.073482428115, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000715 [H]

Pfam domain/function: PF00953 Glycos_transf_4 [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 42216; Mature: 42216

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYGLEVSSDVAGVAGGLLALSYRGAGVPLRELALVGLTAAIITYFATGPVRMLASRLGA
CCCCCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
VAYPRERDVHVTPTPRMGGLAMFLGIVGAVFLASQLPALTRGFVYSTGMPAVLVAGAVIM
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCHHCCCCHHHHHHHHHHH
GIGLIDDRWGLDALTKFAGQITAASVLVTMGVAWSVLYIPVGGVGTIVLDQASSILLTLA
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHH
LTVSIVNAMNFVDGLDGLAAGLGLITALAICMFSVGLLRDHGGDVLYYPPAVISVVLAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
CLGFLPHNFHRAKIFMGDSGSMLIGLMLAAASTTAAGPISQNAYGARDVFALLSPFLLVV
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVMFVPMLDLLLAIVRRTRAGRSAFSPDKMHLHHRLLQIGHSHRRVVLIIYLWVGIGAFG
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AASSIFFNPRDTAAVMLGAIVVAGVATLIPLLRRGDDYYDPDLD
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQYGLEVSSDVAGVAGGLLALSYRGAGVPLRELALVGLTAAIITYFATGPVRMLASRLGA
CCCCCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
VAYPRERDVHVTPTPRMGGLAMFLGIVGAVFLASQLPALTRGFVYSTGMPAVLVAGAVIM
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCHHCCCCHHHHHHHHHHH
GIGLIDDRWGLDALTKFAGQITAASVLVTMGVAWSVLYIPVGGVGTIVLDQASSILLTLA
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHH
LTVSIVNAMNFVDGLDGLAAGLGLITALAICMFSVGLLRDHGGDVLYYPPAVISVVLAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
CLGFLPHNFHRAKIFMGDSGSMLIGLMLAAASTTAAGPISQNAYGARDVFALLSPFLLVV
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVMFVPMLDLLLAIVRRTRAGRSAFSPDKMHLHHRLLQIGHSHRRVVLIIYLWVGIGAFG
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AASSIFFNPRDTAAVMLGAIVVAGVATLIPLLRRGDDYYDPDLD
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Undecaprenyl Phosphate [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Undecaprenyl Phosphate = UMP + Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]