Click here to switch to the map view.

The map label for this gene is sigE [H]

Identifier: 121637150

GI number: 121637150

Start: 1394133

End: 1394906

Strand: Direct

Name: sigE [H]

Synonym: BCG_1281

Alternate gene names: 121637150

Gene position: 1394133-1394906 (Clockwise)

Preceding gene: 121637142

Following gene: 121637151

Centisome position: 31.87

GC content: 63.05

Gene sequence:

>774_bases
ATGGAACTCCTCGGCGGACCCCGGGTTGGGAATACGGAATCGCAACTTTGCGTTGCCGACGGTGACGACTTGCCAACTTA
TTGCAGTGCAAATTCGGAGGATCTCAATATCACGACCATCACGACCTTGAGTCCGACCAGCATGTCTCATCCCCAACAGG
TCCGCGATGACCAGTGGGTGGAGCCGTCTGACCAATTGCAGGGCACCGCCGTATTCGACGCCACCGGGGACAAGGCCACC
ATGCCGTCCTGGGATGAGCTGGTCCGTCAGCACGCCGATCGGGTGTACCGGCTGGCTTATCGGCTCTCCGGCAACCAGCA
CGATGCCGAAGACCTGACCCAGGAGACCTTTATCAGGGTGTTCCGGTCGGTCCAGAATTACCAGCCGGGCACCTTCGAAG
GCTGGCTACACCGCATCACCACCAACTTGTTCCTGGACATGGTCCGCCGCCGGGCTCGCATCCGGATGGAGGCGTTACCC
GAGGACTACGACCGGGTGCCCGCCGATGAGCCCAACCCCGAGCAGATCTACCACGACGCACGGCTGGGCCCTGACCTGCA
GGCTGCCTTGGCCTCGCTGCCGCCGGAGTTTCGTGCCGCGGTGGTGCTGTGTGACATCGAGGGTCTGTCGTACGAGGAGA
TCGGCGCCACACTGGGCGTGAAGCTCGGGACGGTACGTAGCCGGATACACCGCGGACGCCAGGCACTGCGGGACTACCTG
GCAGCGCACCCCGAACATGGCGAGTGCGCAGTTCACGTCAACCCAGTTCGCTGA

Upstream 100 bases:

>100_bases
CTCGATATTCCCTGGACAGCGCAGGTAACGGTATGGTTTCTAAGCCAAAGCTCAGATTGCTCATATATGGCCCATACGCC
GGTACGCGACGGTAATTCCC

Downstream 100 bases:

>100_bases
ACTACTCAACGGCCGCCGAGCGCGTCGGTTCGGCTACCGCATGGTTGCCAATCGGTCCCGAATCCTGGGGTTTTACCGGC
TGGCGATGGTTTTCCGGCAC

Product: RNA polymerase sigma factor SigE

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWVEPSDQLQGTAVFDATGDKAT
MPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMEALP
EDYDRVPADEPNPEQIYHDARLGPDLQAALASLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQALRDYL
AAHPEHGECAVHVNPVR

Sequences:

>Translated_257_residues
MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWVEPSDQLQGTAVFDATGDKAT
MPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMEALP
EDYDRVPADEPNPEQIYHDARLGPDLQAALASLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQALRDYL
AAHPEHGECAVHVNPVR
>Mature_257_residues
MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWVEPSDQLQGTAVFDATGDKAT
MPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMEALP
EDYDRVPADEPNPEQIYHDARLGPDLQAALASLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQALRDYL
AAHPEHGECAVHVNPVR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=172, Percent_Identity=30.8139534883721, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 28878; Mature: 28878

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWV
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCCCCC
EPSDQLQGTAVFDATGDKATMPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRV
CCHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMEALPEDYDRVPADEPNPEQIYHDA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHH
RLGPDLQAALASLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQALRDYL
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAHPEHGECAVHVNPVR
HCCCCCCCEEEEEECCC
>Mature Secondary Structure
MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWV
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHCCCCCC
EPSDQLQGTAVFDATGDKATMPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRV
CCHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMEALPEDYDRVPADEPNPEQIYHDA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHH
RLGPDLQAALASLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQALRDYL
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAHPEHGECAVHVNPVR
HCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]