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The map label for this gene is metB

Identifier: 121637008

GI number: 121637008

Start: 1235366

End: 1236532

Strand: Direct

Name: metB

Synonym: BCG_1137

Alternate gene names: 121637008

Gene position: 1235366-1236532 (Clockwise)

Preceding gene: 121637007

Following gene: 121637011

Centisome position: 28.24

GC content: 64.78

Gene sequence:

>1167_bases
ATGAGCGAAGACCGCACGGGACACCAGGGAATCAGCGGACCGGCCACCCGCGCCATCCACGCTGGCTACCGCCCGGATCC
GGCGACCGGGGCGGTGAACGTGCCGATCTACGCCAGCAGCACCTTCGCCCAAGACGGCGTCGGCGGTCTGCGTGGCGGTT
TCGAATACGCACGCACCGGCAACCCCACCCGGGCCGCATTGGAGGCCTCGCTGGCGGCAGTCGAGGAGGGTGCTTTCGCG
CGGGCATTCAGTTCCGGGATGGCCGCGACCGACTGCGCCCTGCGGGCGATGTTACGGCCCGGAGACCACGTCGTCATTCC
CGATGACGCCTACGGCGGCACATTCCGGTTGATAGACAAGGTGTTCACCCGGTGGGATGTCCAGTACACGCCGGTGCGGC
TTGCCGATCTGGATGCGGTGGGTGCCGCGATTACTCCGCGCACCCGGCTGATTTGGGTGGAGACGCCCACCAATCCGCTA
CTGTCGATCGCCGATATCACGGCCATTGCCGAGCTGGGCACAGACAGATCGGCAAAAGTATTGGTGGACAATACCTTTGC
CTCACCCGCGTTGCAGCAGCCGTTGCGGCTGGGCGCCGATGTGGTGTTGCACTCGACTACCAAGTACATCGGCGGCCATT
CCGACGTGGTGGGAGGTGCGCTGGTCACCAACGACGAAGAGCTGGACGAGGAGTTCGCTTTCTTGCAGAACGGCGCCGGC
GCGGTGCCCGGACCATTCGACGCCTACCTGACCATGCGCGGCCTGAAGACCTTGGTGCTGCGGATGCAGCGGCACAGTGA
AAATGCCTGTGCGGTAGCGGAATTCCTCGCTGATCATCCGTCGGTGAGTTCTGTGTTGTATCCGGGTTTGCCCAGTCATC
CCGGGCATGAGATTGCCGCGCGACAGATGCGCGGCTTCGGCGGCATGGTTTCGGTGCGGATGCGGGCCGGTCGGCGTGCG
GCGCAGGACCTGTGTGCCAAGACCCGCGTCTTCATCCTGGCCGAGTCGCTGGGTGGGGTGGAGTCGCTGATCGAACATCC
CAGCGCCATGACCCATGCGTCGACGGCCGGTTCGCAATTGGAGGTGCCCGACGATCTGGTGCGGCTTTCGGTCGGTATCG
AAGACATTGCCGACCTGCTCGGCGATCTCGAACAGGCCCTGGGTTAA

Upstream 100 bases:

>100_bases
CCGCTGTGGGACGCTAAACGGCAAACGTTGGCGGACAAGATCATGACGACGGTGTGCGTGCCGATCTGATCCGGGACTGC
ACTGCCCACCCGACCGTCCG

Downstream 100 bases:

>100_bases
CTACCGCGAGCAGACGCGAAAGCACCCCAAAACCGCCGGTTTGGGGGCTTCTGCGTCTGCTCGCGGGTACCTAGGAGTGG
TACGGCTCGGCGCTGACTAG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSEDRTGHQGISGPATRAIHAGYRPDPATGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRAALEASLAAVEEGAFA
RAFSSGMAATDCALRAMLRPGDHVVIPDDAYGGTFRLIDKVFTRWDVQYTPVRLADLDAVGAAITPRTRLIWVETPTNPL
LSIADITAIAELGTDRSAKVLVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDEEFAFLQNGAG
AVPGPFDAYLTMRGLKTLVLRMQRHSENACAVAEFLADHPSVSSVLYPGLPSHPGHEIAARQMRGFGGMVSVRMRAGRRA
AQDLCAKTRVFILAESLGGVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDIADLLGDLEQALG

Sequences:

>Translated_388_residues
MSEDRTGHQGISGPATRAIHAGYRPDPATGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRAALEASLAAVEEGAFA
RAFSSGMAATDCALRAMLRPGDHVVIPDDAYGGTFRLIDKVFTRWDVQYTPVRLADLDAVGAAITPRTRLIWVETPTNPL
LSIADITAIAELGTDRSAKVLVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDEEFAFLQNGAG
AVPGPFDAYLTMRGLKTLVLRMQRHSENACAVAEFLADHPSVSSVLYPGLPSHPGHEIAARQMRGFGGMVSVRMRAGRRA
AQDLCAKTRVFILAESLGGVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDIADLLGDLEQALG
>Mature_387_residues
SEDRTGHQGISGPATRAIHAGYRPDPATGAVNVPIYASSTFAQDGVGGLRGGFEYARTGNPTRAALEASLAAVEEGAFAR
AFSSGMAATDCALRAMLRPGDHVVIPDDAYGGTFRLIDKVFTRWDVQYTPVRLADLDAVGAAITPRTRLIWVETPTNPLL
SIADITAIAELGTDRSAKVLVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDEEFAFLQNGAGA
VPGPFDAYLTMRGLKTLVLRMQRHSENACAVAEFLADHPSVSSVLYPGLPSHPGHEIAARQMRGFGGMVSVRMRAGRRAA
QDLCAKTRVFILAESLGGVESLIEHPSAMTHASTAGSQLEVPDDLVRLSVGIEDIADLLGDLEQALG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=47.6190476190476, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=44.1798941798942, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=42.4403183023873, Blast_Score=261, Evalue=7e-70,
Organism=Escherichia coli, GI1790375, Length=376, Percent_Identity=43.3510638297872, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=30.7888040712468, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17538055, Length=379, Percent_Identity=47.2295514511873, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17560056, Length=379, Percent_Identity=45.1187335092348, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71980708, Length=376, Percent_Identity=38.8297872340425, Blast_Score=257, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=47.7922077922078, Blast_Score=320, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6321254, Length=400, Percent_Identity=31.25, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6321170, Length=334, Percent_Identity=29.940119760479, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=30.715935334873, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=391, Percent_Identity=29.923273657289, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=48.9528795811518, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METB_MYCBO (P66876)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854763.1
- ProteinModelPortal:   P66876
- SMR:   P66876
- EnsemblBacteria:   EBMYCT00000014273
- GeneID:   1090439
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1108
- GeneTree:   EBGT00050000016651
- HOGENOM:   HBG754729
- OMA:   RTEIFIL
- ProtClustDB:   PRK07811
- BioCyc:   MBOV233413:MB1108-MONOMER
- BRENDA:   2.5.1.48
- GO:   GO:0005737
- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11808
- PIRSF:   PIRSF001434

Pfam domain/function: PF01053 Cys_Met_Meta_PP; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.5.1.48

Molecular weight: Translated: 40983; Mature: 40852

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00868 CYS_MET_METAB_PP; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDRTGHQGISGPATRAIHAGYRPDPATGAVNVPIYASSTFAQDGVGGLRGGFEYARTG
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHCCC
NPTRAALEASLAAVEEGAFARAFSSGMAATDCALRAMLRPGDHVVIPDDAYGGTFRLIDK
CCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
VFTRWDVQYTPVRLADLDAVGAAITPRTRLIWVETPTNPLLSIADITAIAELGTDRSAKV
HHHHCCCEECCEEEECHHHHCCEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
LVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDEEFAFLQNGAG
EEECCCCCHHHHHHHHCCCCEEEECHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCC
AVPGPFDAYLTMRGLKTLVLRMQRHSENACAVAEFLADHPSVSSVLYPGLPSHPGHEIAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHEECCCCCCCCCHHHHH
RQMRGFGGMVSVRMRAGRRAAQDLCAKTRVFILAESLGGVESLIEHPSAMTHASTAGSQL
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEHHHCCHHHHHHCCCHHHHHHCCCCCC
EVPDDLVRLSVGIEDIADLLGDLEQALG
CCCHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEDRTGHQGISGPATRAIHAGYRPDPATGAVNVPIYASSTFAQDGVGGLRGGFEYARTG
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHCCC
NPTRAALEASLAAVEEGAFARAFSSGMAATDCALRAMLRPGDHVVIPDDAYGGTFRLIDK
CCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
VFTRWDVQYTPVRLADLDAVGAAITPRTRLIWVETPTNPLLSIADITAIAELGTDRSAKV
HHHHCCCEECCEEEECHHHHCCEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEE
LVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGGALVTNDEELDEEFAFLQNGAG
EEECCCCCHHHHHHHHCCCCEEEECHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCC
AVPGPFDAYLTMRGLKTLVLRMQRHSENACAVAEFLADHPSVSSVLYPGLPSHPGHEIAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHEECCCCCCCCCHHHHH
RQMRGFGGMVSVRMRAGRRAAQDLCAKTRVFILAESLGGVESLIEHPSAMTHASTAGSQL
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHEEEEHHHCCHHHHHHCCCHHHHHHCCCCCC
EVPDDLVRLSVGIEDIADLLGDLEQALG
CCCHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972