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The map label for this gene is prrB

Identifier: 121636825

GI number: 121636825

Start: 1034975

End: 1036315

Strand: Reverse

Name: prrB

Synonym: BCG_0954c

Alternate gene names: 121636825

Gene position: 1036315-1034975 (Counterclockwise)

Preceding gene: 121636826

Following gene: 121636821

Centisome position: 23.69

GC content: 68.53

Gene sequence:

>1341_bases
ATGAATATTCTGTCGCGGATCTTCGCCCGTACGCCCTCGCTGCGAACCCGGGTGGTGGTCGCGACGGCGATCGGCGCCGC
GATCCCGGTACTCATTGTCGGCACCGTCGTCTGGGTCGGGATCACCAACGACCGCAAGGAGCGGCTGGACCGCCGGCTCG
ACGAGGCCGCGGGTTTCGCGATCCCGTTCGTGCCGCGTGGCCTCGACGAGATCCCGCGCTCACCGAACGACCAGGACGCC
CTCATCACGGTCCGCCGTGGCAACGTGATCAAGTCGAATTCCGACATCACGCTGCCCAAACTGCAAGACGACTACGCCGA
CACCTACGTCCGCGGGGTGCGCTACCGCGTGCGCACGGTGGAGATCCCCGGGCCCGAGCCGACGTCGGTGGCGGTGGGCG
CGACGTATGACGCCACCGTAGCCGAGACCAACAACCTGCACCGCCGGGTGCTGCTGATCTGTACCTTCGCCATCGGCGCG
GCGGCGGTGTTCGCCTGGCTGTTGGCCGCGTTCGCGGTACGCCCGTTCAAGCAGCTCGCCGAGCAGACCCGGTCCATCGA
CGCGGGCGACGAGGCGCCACGCGTGGAAGTACACGGCGCCAGCGAAGCCATCGAGATCGCCGAGGCGATGCGCGGCATGC
TGCAGCGCATCTGGAACGAGCAGAACCGAACCAAGGAGGCGCTTGCGTCGGCGCGTGACTTCGCGGCGGTGTCCTCCCAC
GAGCTGCGCACTCCGCTGACCGCGATGCGCACCAACCTGGAGGTGCTGTCCACCCTGGATCTGCCCGACGACCAGCGCAA
AGAAGTGCTCAACGACGTCATCCGCACCCAGTCGCGCATTGAGGCCACCCTGAGCGCGCTGGAGCGGTTGGCCCAGGGCG
AACTGTCGACCTCGGACGATCACGTGCCGGTCGATATCACCGACCTGCTCGACCGCGCCGCCCACGACGCGGCCCGGATC
TACCCCGATCTCGATGTATCGCTGGTGCCGTCGCCGACCTGCATCATCGTGGGTTTGCCGGCCGGGTTGCGCCTGGCCGT
CGACAACGCCATCGCCAACGCGGTCAAGCACGGCGGCGCCACCCTGGTTCAACTCTCCGCGGTCAGCTCGCGGGCCGGCG
TGGAGATCGCCATCGACGACAACGGCAGCGGAGTGCCCGAAGGTGAGCGCCAAGTGGTGTTCGAGCGGTTCTCCCGCGGG
TCCACGGCATCTCATTCGGGGTCGGGTCTCGGGCTGGCGTTGGTGGCCCAACAGGCCCAGTTGCACGGCGGCACCGCGTC
GCTGGAGAACAGCCCGCTGGGTGGCGCGCGCCTCGTGTTGCGCCTTCCCGGGCCCAGTTAG

Upstream 100 bases:

>100_bases
GGGTACCTGCGGCGCAAACTGGAGGCCGGCGGTGGCCCTAGGCTGCTGCATACCGTCCGCGGAGTCGGATTCGTGCTGCG
TATGCAGTGACGGGGCATGC

Downstream 100 bases:

>100_bases
CCCTGCGGCCCTGACCTCATGTCCGCCGTGTGCTCTTGCGCCACGGCGTGAAAAACGCCCGCGCTGCCGACTCCTCGTCC
TGGAACCAAACCTGGGCGAC

Product: two component sensor histidine kinase prrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRRLDEAAGFAIPFVPRGLDEIPRSPNDQDA
LITVRRGNVIKSNSDITLPKLQDDYADTYVRGVRYRVRTVEIPGPEPTSVAVGATYDATVAETNNLHRRVLLICTFAIGA
AAVFAWLLAAFAVRPFKQLAEQTRSIDAGDEAPRVEVHGASEAIEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
ELRTPLTAMRTNLEVLSTLDLPDDQRKEVLNDVIRTQSRIEATLSALERLAQGELSTSDDHVPVDITDLLDRAAHDAARI
YPDLDVSLVPSPTCIIVGLPAGLRLAVDNAIANAVKHGGATLVQLSAVSSRAGVEIAIDDNGSGVPEGERQVVFERFSRG
STASHSGSGLGLALVAQQAQLHGGTASLENSPLGGARLVLRLPGPS

Sequences:

>Translated_446_residues
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRRLDEAAGFAIPFVPRGLDEIPRSPNDQDA
LITVRRGNVIKSNSDITLPKLQDDYADTYVRGVRYRVRTVEIPGPEPTSVAVGATYDATVAETNNLHRRVLLICTFAIGA
AAVFAWLLAAFAVRPFKQLAEQTRSIDAGDEAPRVEVHGASEAIEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
ELRTPLTAMRTNLEVLSTLDLPDDQRKEVLNDVIRTQSRIEATLSALERLAQGELSTSDDHVPVDITDLLDRAAHDAARI
YPDLDVSLVPSPTCIIVGLPAGLRLAVDNAIANAVKHGGATLVQLSAVSSRAGVEIAIDDNGSGVPEGERQVVFERFSRG
STASHSGSGLGLALVAQQAQLHGGTASLENSPLGGARLVLRLPGPS
>Mature_446_residues
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRRLDEAAGFAIPFVPRGLDEIPRSPNDQDA
LITVRRGNVIKSNSDITLPKLQDDYADTYVRGVRYRVRTVEIPGPEPTSVAVGATYDATVAETNNLHRRVLLICTFAIGA
AAVFAWLLAAFAVRPFKQLAEQTRSIDAGDEAPRVEVHGASEAIEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
ELRTPLTAMRTNLEVLSTLDLPDDQRKEVLNDVIRTQSRIEATLSALERLAQGELSTSDDHVPVDITDLLDRAAHDAARI
YPDLDVSLVPSPTCIIVGLPAGLRLAVDNAIANAVKHGGATLVQLSAVSSRAGVEIAIDDNGSGVPEGERQVVFERFSRG
STASHSGSGLGLALVAQQAQLHGGTASLENSPLGGARLVLRLPGPS

Specific function: Member of the two-component regulatory system prrB/prrA. Involved specifically in early intracellular multiplication of Mycobacterium. Probably phosphorylates prrA

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1790346, Length=208, Percent_Identity=31.25, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=29.6819787985866, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=30.4166666666667, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1789808, Length=306, Percent_Identity=27.1241830065359, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=299, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789403, Length=461, Percent_Identity=26.4642082429501, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=25.609756097561, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787374, Length=276, Percent_Identity=25.3623188405797, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787894, Length=227, Percent_Identity=30.8370044052863, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=334, Percent_Identity=24.251497005988, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=220, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786783, Length=336, Percent_Identity=22.3214285714286, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=24.4186046511628, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRRB_MYCBO (P0A5Z9)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854583.1
- ProteinModelPortal:   P0A5Z9
- SMR:   P0A5Z9
- EnsemblBacteria:   EBMYCT00000016853
- GeneID:   1092847
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0926c
- GeneTree:   EBGT00050000014826
- HOGENOM:   HBG566771
- OMA:   QIRTKEA
- ProtClustDB:   CLSK790821
- BioCyc:   MBOV233413:MB0926C-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00304
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 47830; Mature: 47830

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x14a88210)-; HASH(0x1440603c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRRLDEAAGFA
CCHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCC
IPFVPRGLDEIPRSPNDQDALITVRRGNVIKSNSDITLPKLQDDYADTYVRGVRYRVRTV
CCCCCCCHHHCCCCCCCCCEEEEEECCCEEECCCCEECCCCCHHHHHHHHHHCEEEEEEE
EIPGPEPTSVAVGATYDATVAETNNLHRRVLLICTFAIGAAAVFAWLLAAFAVRPFKQLA
ECCCCCCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQTRSIDAGDEAPRVEVHGASEAIEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
HHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
ELRTPLTAMRTNLEVLSTLDLPDDQRKEVLNDVIRTQSRIEATLSALERLAQGELSTSDD
HHHCCHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HVPVDITDLLDRAAHDAARIYPDLDVSLVPSPTCIIVGLPAGLRLAVDNAIANAVKHGGA
CCCEEHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCEEHHHHHHHHHHHCCCC
TLVQLSAVSSRAGVEIAIDDNGSGVPEGERQVVFERFSRGSTASHSGSGLGLALVAQQAQ
EEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LHGGTASLENSPLGGARLVLRLPGPS
HCCCCCCCCCCCCCCEEEEEECCCCH
>Mature Secondary Structure
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRRLDEAAGFA
CCHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCC
IPFVPRGLDEIPRSPNDQDALITVRRGNVIKSNSDITLPKLQDDYADTYVRGVRYRVRTV
CCCCCCCHHHCCCCCCCCCEEEEEECCCEEECCCCEECCCCCHHHHHHHHHHCEEEEEEE
EIPGPEPTSVAVGATYDATVAETNNLHRRVLLICTFAIGAAAVFAWLLAAFAVRPFKQLA
ECCCCCCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQTRSIDAGDEAPRVEVHGASEAIEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
HHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
ELRTPLTAMRTNLEVLSTLDLPDDQRKEVLNDVIRTQSRIEATLSALERLAQGELSTSDD
HHHCCHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HVPVDITDLLDRAAHDAARIYPDLDVSLVPSPTCIIVGLPAGLRLAVDNAIANAVKHGGA
CCCEEHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCEEHHHHHHHHHHHCCCC
TLVQLSAVSSRAGVEIAIDDNGSGVPEGERQVVFERFSRGSTASHSGSGLGLALVAQQAQ
EEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LHGGTASLENSPLGGARLVLRLPGPS
HCCCCCCCCCCCCCCEEEEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972