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The map label for this gene is grpE

Identifier: 121636265

GI number: 121636265

Start: 452636

End: 453343

Strand: Direct

Name: grpE

Synonym: BCG_0390

Alternate gene names: 121636265

Gene position: 452636-453343 (Clockwise)

Preceding gene: 121636264

Following gene: 121636266

Centisome position: 10.35

GC content: 65.4

Gene sequence:

>708_bases
GTGACGGACGGAAATCAAAAGCCGGATGGCAATTCGGGCGAACAGGTAACCGTCACTGACAAGCGGCGGATCGATCCCGA
GACGGGTGAAGTGCGGCACGTCCCTCCCGGCGACATGCCGGGAGGGACGGCTGCGGCCGATGCGGCGCACACCGAAGACA
AGGTCGCCGAGCTGACCGCCGATCTGCAACGCGTGCAGGCCGACTTCGCCAACTACCGTAAGCGGGCGTTGCGCGATCAG
CAGGCGGCCGCTGACCGAGCCAAGGCCAGCGTTGTCAGCCAATTGCTGGGTGTACTGGACGATCTCGAGCGGGCGCGCAA
GCACGGCGATTTGGAGTCGGGTCCACTGAAGTCGGTCGCCGACAAGCTAGACAGCGCGTTGACCGGGCTGGGTCTGGTGG
CGTTCGGTGCCGAGGGCGAGGATTTCGACCCCGTGCTGCACGAAGCGGTGCAACACGAGGGCGACGGCGGGCAGGGGTCC
AAGCCGGTAATCGGCACCGTCATGCGGCAGGGCTACCAACTGGGTGAGCAGGTGCTGCGGCACGCCTTGGTCGGCGTCGT
CGACACGGTGGTCGTCGACGCGGCCGAACTGGAGTCAGTCGACGACGGCACTGCGGTCGCAGATACCGCCGAAAACGATC
AAGCTGACCAGGGCAATAGCGCCGACACCTTGGGCGAACAGGCAGAATCAGAACCGTCGGGCAGTTAA

Upstream 100 bases:

>100_bases
CTGGCGCTGCCCACCCCGGCGGCGAGCCGGGCGGTGCCCACCCCGGCTCGGCTGATGACGTTGTGGACGCGGAGGTGGTC
GACGACGGCCGGGAGGCCAA

Downstream 100 bases:

>100_bases
CAACAAAAGAGGAAGGCGAGAGGGGGTGACGCGACATGGCCCAAAGGGAATGGGTCGAAAAAGACTTCTACCAGGAGCTG
GGCGTCTCCTCTGATGCCAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTDGNQKPDGNSGEQVTVTDKRRIDPETGEVRHVPPGDMPGGTAAADAAHTEDKVAELTADLQRVQADFANYRKRALRDQ
QAAADRAKASVVSQLLGVLDDLERARKHGDLESGPLKSVADKLDSALTGLGLVAFGAEGEDFDPVLHEAVQHEGDGGQGS
KPVIGTVMRQGYQLGEQVLRHALVGVVDTVVVDAAELESVDDGTAVADTAENDQADQGNSADTLGEQAESEPSGS

Sequences:

>Translated_235_residues
MTDGNQKPDGNSGEQVTVTDKRRIDPETGEVRHVPPGDMPGGTAAADAAHTEDKVAELTADLQRVQADFANYRKRALRDQ
QAAADRAKASVVSQLLGVLDDLERARKHGDLESGPLKSVADKLDSALTGLGLVAFGAEGEDFDPVLHEAVQHEGDGGQGS
KPVIGTVMRQGYQLGEQVLRHALVGVVDTVVVDAAELESVDDGTAVADTAENDQADQGNSADTLGEQAESEPSGS
>Mature_234_residues
TDGNQKPDGNSGEQVTVTDKRRIDPETGEVRHVPPGDMPGGTAAADAAHTEDKVAELTADLQRVQADFANYRKRALRDQQ
AAADRAKASVVSQLLGVLDDLERARKHGDLESGPLKSVADKLDSALTGLGLVAFGAEGEDFDPVLHEAVQHEGDGGQGSK
PVIGTVMRQGYQLGEQVLRHALVGVVDTVVVDAAELESVDDGTAVADTAENDQADQGNSADTLGEQAESEPSGS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_MYCBO (Q7U272)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854022.1
- ProteinModelPortal:   Q7U272
- SMR:   Q7U272
- EnsemblBacteria:   EBMYCT00000016241
- GeneID:   1091249
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0359
- GeneTree:   EBGT00050000017239
- HOGENOM:   HBG732630
- OMA:   IGRARDH
- ProtClustDB:   PRK14142
- BioCyc:   MBOV233413:MB0359-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24559; Mature: 24428

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGNQKPDGNSGEQVTVTDKRRIDPETGEVRHVPPGDMPGGTAAADAAHTEDKVAELTA
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
DLQRVQADFANYRKRALRDQQAAADRAKASVVSQLLGVLDDLERARKHGDLESGPLKSVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DKLDSALTGLGLVAFGAEGEDFDPVLHEAVQHEGDGGQGSKPVIGTVMRQGYQLGEQVLR
HHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HALVGVVDTVVVDAAELESVDDGTAVADTAENDQADQGNSADTLGEQAESEPSGS
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDGNQKPDGNSGEQVTVTDKRRIDPETGEVRHVPPGDMPGGTAAADAAHTEDKVAELTA
CCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
DLQRVQADFANYRKRALRDQQAAADRAKASVVSQLLGVLDDLERARKHGDLESGPLKSVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DKLDSALTGLGLVAFGAEGEDFDPVLHEAVQHEGDGGQGSKPVIGTVMRQGYQLGEQVLR
HHHHHHHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HALVGVVDTVVVDAAELESVDDGTAVADTAENDQADQGNSADTLGEQAESEPSGS
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972