Click here to switch to the map view.

The map label for this gene is dnaK [C]

Identifier: 121636227

GI number: 121636227

Start: 411483

End: 413345

Strand: Direct

Name: dnaK [C]

Synonym: BCG_0352

Alternate gene names: 121636227

Gene position: 411483-413345 (Clockwise)

Preceding gene: 121636226

Following gene: 121636228

Centisome position: 9.41

GC content: 65.7

Gene sequence:

>1863_bases
ATGTACGACCCGCTGGGGTTGTCGATCGGGACCACAAACCTGGTCGCGGCGGGTAACGGAGGTCCGCCGGTTACTCGTCG
CGCCGTGCTGACCCTGTACCCGCATTGCGCACCGAAAATCGGTGTGCCTAGCCAGAACCCGAACTTGATCGAGCCGGGCG
CCCTAATGAGCGGCTTTGTTGAGCGCATTGGAGATGCGGTGGCGCTGGTGTCTCCCGACGGATCCGTGCACGATCCAGAC
CTCTTGCTGGTCGAGGCGCTGGATGCGATGGTGCTGACCGCCGGTGCGGACGCGAGTTCCTCGGAGATCGCCATTGCCGT
TCCCGCGCATTGGAAGCCCGGAGCTGTACACGCACTGCGTAACGGTTTGCGGACGCACGTCGGCTTCGTCCGCAGCGGCA
TGGCGCCGCGCCTGGTTTCCGATGCGATCGCGGCGTTGACCGCGGTGAACTCGGAATTGGGCCTGCCCCACGGCGGTGTG
GTGGGGTTGCTTGATTTCGGTGGCTCCGCGACTTACGTCACCTTGGTGGAGACCAAGTCGGATTCCAGGACGTCGGATTT
CCAGCCCGTTAGTGCCACGGCACGGTACCAGGACTTTTCCGGTAGTCAGATCGACCAGGCTTTGCTGCTTCGGGTCATCG
ACCAATTCGGGTACGGCGATGACGTCGATCCGGCCAGTACCGCCGCGGTCGGGCAACTCGGCCAACTCAGGGAGCAGTGC
CGTGCGGCAAAGGAACGACTGTCCACCGACGTTGCCACGGAATTGTTCGCTGAGCTTGCCGGGTGCAGCTCGAGCATCGA
GATGACTCGGGAACAGCTCGAAGACCTGATCCAGGATCCATTGACCGGCTTCATCTACGCGTTCGACGACATGCTGGCGC
GCCACAACGCGAGCTGGGCGGATCTCGCGGCGGTGGTCACCGTCGGCGGTGGTGCCAATATTCCCCTTGTGACTCAACGT
CTTTCGTTCCACACTCGTCGACCTGTGCTGACCGCGTCGCAACCCGGGTGCGCGGCGGCGATGGGTGCGTTGCTGCTCGC
CAACCGTGGGGGAGAGCGCGATTCGCGAACGCGGACGTCCATCGGCCTCGCCACGGCCGCAGCCGCCGGCACCAGTGTCA
TCGAGCTGCCGGCCGGCGACGTCATGGTCATCGACCATGAGGCCTTGACCGATCGCGAGTTGGCCTGGTCGCAGACCGAC
TTCCCAAGCGAAGCTCCGGCGCGTTTCGAGGGCGACTCGTATAACGAAGGCGGCCCCTGCTGGTCGATGCGTCTGAACGC
GGTCGAGCCCCCCAAAGGACCAGCGTGGCGGCGAATCCGGGTGTCGCAGTTGCTCATCGGGGTGTCGGCGGTAGTGGCCA
TGACCGCGATCGGGGGCGTGGCATTGACGTTGACAGCCATCGAGAGACGCCCAAGCCCGCTACCAACCCCAATTGTGCCC
GGCCTGGCCCCGATGCCGCCCGGATCCGTCGTGCCTAGCTCGCGCGCACCGACCCCGCCGCCACCGCCGTCGACCGTTGC
GCCGCTTCCCAGTGCGGCACCGGCCCCGACGACGGTCGCGCCGGCACCGCCGCCGCCCACACAGGTGGTGACGACCACGA
CAGCGCCACCCGTCACCACGACGCCGAGGCCGTCGCCGACCACCACAACGACCACCGCGCCACCGTCGACAACGACGACA
ACCGAGCTGCCGGTGACGACCACTTCGACGATTCCAACGATTCCGACGACTACGACGACGGTGAAGATGACCACGGAGTG
GTTGCACGTCCCGTTTTTGCCCGTTCCGATCCCGGTCCCGATTCCGCAAAATCCGGGTGCCGGCGAACCGCAGAACCCGT
TCGGAAGCCTTGGCTCTGGGTGA

Upstream 100 bases:

>100_bases
TCGACCGCATACCAATGAGGTTGTCACCGAACCGTATACGGCCCACCCGCCGCTATGGTTAACGCTGGCCACCGACCCCT
ATTGACGAAAGCCTTCCGCT

Downstream 100 bases:

>100_bases
GCCGCGTTCCCCGGAGCTGGCCCCGTCGGTGTCAGGTCCGTAGTATCGGTATGGGTTGCTGAGGAGGTCGTGTGGGCGAC
TATGGTCCGTTTGGATTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: None

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFVERIGDAVALVSPDGSVHDPD
LLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALRNGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGGV
VGLLDFGGSATYVTLVETKSDSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWADLAAVVTVGGGANIPLVTQR
LSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTSIGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTD
FPSEAPARFEGDSYNEGGPCWSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTTTPRPSPTTTTTTAPPSTTTT
TELPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVPIPQNPGAGEPQNPFGSLGSG

Sequences:

>Translated_620_residues
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFVERIGDAVALVSPDGSVHDPD
LLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALRNGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGGV
VGLLDFGGSATYVTLVETKSDSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWADLAAVVTVGGGANIPLVTQR
LSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTSIGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTD
FPSEAPARFEGDSYNEGGPCWSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTTTPRPSPTTTTTTAPPSTTTT
TELPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVPIPQNPGAGEPQNPFGSLGSG
>Mature_620_residues
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFVERIGDAVALVSPDGSVHDPD
LLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALRNGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGGV
VGLLDFGGSATYVTLVETKSDSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWADLAAVVTVGGGANIPLVTQR
LSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTSIGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTD
FPSEAPARFEGDSYNEGGPCWSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTTTPRPSPTTTTTTAPPSTTTT
TELPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVPIPQNPGAGEPQNPFGSLGSG

Specific function: Plays An Essential Role In The Initiation Of Phage Lambda DNA Replication, Where It Acts In An ATP-Dependent Fashion With The DNAj Protein To Release Lambda O And P Proteins From The Preprimosomal Complex. DNAk Is Also Involved In Chromosomal DNA Replica

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 64130; Mature: 64130

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFV
CCCCCCCEECCCEEEEECCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
ERIGDAVALVSPDGSVHDPDLLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALR
HHHCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCCCHHHHHH
NGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGGVVGLLDFGGSATYVTLVETKS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEEECC
DSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHH
DLAAVVTVGGGANIPLVTQRLSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTS
HEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHH
IGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTDFPSEAPARFEGDSYNEGGPC
HHHHHHHHCCCEEEEECCCCEEEEECHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCE
WSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCC
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
TPRPSPTTTTTTAPPSTTTTTELPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVP
CCCCCCCCCCCCCCCCCCCCEECCEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
IPQNPGAGEPQNPFGSLGSG
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYDPLGLSIGTTNLVAAGNGGPPVTRRAVLTLYPHCAPKIGVPSQNPNLIEPGALMSGFV
CCCCCCCEECCCEEEEECCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
ERIGDAVALVSPDGSVHDPDLLLVEALDAMVLTAGADASSSEIAIAVPAHWKPGAVHALR
HHHCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCCCHHHHHH
NGLRTHVGFVRSGMAPRLVSDAIAALTAVNSELGLPHGGVVGLLDFGGSATYVTLVETKS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEEECC
DSRTSDFQPVSATARYQDFSGSQIDQALLLRVIDQFGYGDDVDPASTAAVGQLGQLREQC
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
RAAKERLSTDVATELFAELAGCSSSIEMTREQLEDLIQDPLTGFIYAFDDMLARHNASWA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHH
DLAAVVTVGGGANIPLVTQRLSFHTRRPVLTASQPGCAAAMGALLLANRGGERDSRTRTS
HEEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHH
IGLATAAAAGTSVIELPAGDVMVIDHEALTDRELAWSQTDFPSEAPARFEGDSYNEGGPC
HHHHHHHHCCCEEEEECCCCEEEEECHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCE
WSMRLNAVEPPKGPAWRRIRVSQLLIGVSAVVAMTAIGGVALTLTAIERRPSPLPTPIVP
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCC
GLAPMPPGSVVPSSRAPTPPPPPSTVAPLPSAAPAPTTVAPAPPPPTQVVTTTTAPPVTT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
TPRPSPTTTTTTAPPSTTTTTELPVTTTSTIPTIPTTTTTVKMTTEWLHVPFLPVPIPVP
CCCCCCCCCCCCCCCCCCCCEECCEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCC
IPQNPGAGEPQNPFGSLGSG
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA