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The map label for this gene is 121636133

Identifier: 121636133

GI number: 121636133

Start: 293999

End: 294772

Strand: Direct

Name: 121636133

Synonym: BCG_0258

Alternate gene names: NA

Gene position: 293999-294772 (Clockwise)

Preceding gene: 121636132

Following gene: 121636137

Centisome position: 6.72

GC content: 62.92

Gene sequence:

>774_bases
GTGAAACGGCTCAGCGGCTGGGACGCGGTACTGCTTTACAGCGAGACCCCGAATGTGCACATGCACACACTCAAGGTCGC
CGTGATCGAATTGGATTCGGACAGACAGGAATTCGGTGTCGACGCGTTTCGCGAGGTGATCGCTGGCCGGCTGCATAAGC
TTGAGCCATTGGGCTATCAGCTGGTTGATGTCCCGTTGAAGTTCCATCACCCGATGTGGCGGGAGCACTGCCAGGTCGAT
CTCAACTACCACATCCGGCCGTGGCGGTTGCGCGCCCCGGGGGGTCGGCGCGAACTCGACGAGGCGGTCGGAGAAATCGC
CAGCACCCCGCTGAACCGCGACCACCCGCTGTGGGAGATGTACTTCGTTGAGGGGCTTGCCAACCACCGGATCGCGGTGG
TTGCCAAAATTCACCATGCGTTGGCTGACGGTGTTGCCTCGGCAAACATGATGGCACGGGGGATGGATCTGCTGCCGGGA
CCGGAGGTCGGCCGCTATGTGCCTGACCCCGCTCCTACCAAGCGGCAGTTGCTGTCCGCGGCGTTCATCGACCACTTGCG
CCACCTCGGCCGGATTCCTGCAACCATCCGGTACACCACGCAGGGTCTAGGCCGGGTGCGACGTAGCTCGCGCAAGCTCT
CACCCGCACTGACCATGCCATTTACCCCGCCACCGACGTTCATGAATCGGATAAAGAAGCCGTTGTCCAAGCCCTCGGGA
CGGCCGCCGCCGCACACCAACCGAGCGCCCTCCTCGATGCCCTTGGCGATGTAG

Upstream 100 bases:

>100_bases
CTGTTTCTCAACCAGGTTCATCGCAGCCGGGCACAGTTCGCGAAAGAGGTCATCCAAACGCCGGCCAATTGTATGGTCGC
CCTATGAGTAGGGGGCTGCG

Downstream 100 bases:

>100_bases
CGCTTCAACGCGAGTCCGCTGCTTCTCCGAGATCAGCGGCCCGATCTGAGCTGCCGGGTCCGACGGCGGGCCCACCGGGA
GAGCCGTTACGAAATTAGTT

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv0221/MT0231 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQLVDVPLKFHHPMWREHCQVD
LNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEMYFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPG
PEVGRYVPDPAPTKRQLLSAAFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNRIKKPLSKPSG
RPPPHTNRAPSSMPLAM

Sequences:

>Translated_257_residues
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQLVDVPLKFHHPMWREHCQVD
LNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEMYFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPG
PEVGRYVPDPAPTKRQLLSAAFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNRIKKPLSKPSG
RPPPHTNRAPSSMPLAM
>Mature_257_residues
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQLVDVPLKFHHPMWREHCQVD
LNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEMYFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPG
PEVGRYVPDPAPTKRQLLSAAFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNRIKKPLSKPSG
RPPPHTNRAPSSMPLAM

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 29026; Mature: 29026

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQ
CCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCHHHHCHHHHHHHHHHHHHHCCCCCCE
LVDVPLKFHHPMWREHCQVDLNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEM
EEECCHHHCCHHHHHHCEEEECEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHH
YFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPGPEVGRYVPDPAPTKRQLLSA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
AFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNRIKKPLSKPSG
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCC
RPPPHTNRAPSSMPLAM
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQ
CCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCHHHHCHHHHHHHHHHHHHHCCCCCCE
LVDVPLKFHHPMWREHCQVDLNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEM
EEECCHHHCCHHHHHHCEEEECEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHH
YFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPGPEVGRYVPDPAPTKRQLLSA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
AFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNRIKKPLSKPSG
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCC
RPPPHTNRAPSSMPLAM
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]