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The map label for this gene is hyfB [C]

Identifier: 121635996

GI number: 121635996

Start: 120141

End: 122069

Strand: Direct

Name: hyfB [C]

Synonym: BCG_0116

Alternate gene names: 121635996

Gene position: 120141-122069 (Clockwise)

Preceding gene: 121635995

Following gene: 121635997

Centisome position: 2.75

GC content: 71.18

Gene sequence:

>1929_bases
ATGACCGCTGCACCGACGGCCGGCGGGGTCGTCACTTCGGGCGTGGGCGTTGCCGGGGTCGGCGTGGGGTTGCTGGGCAT
GTTTGGACCGGTGCGTGTAGTGCACGTCGGTTGGCTGCTTCCGCTGTCCGGCGTGCACATCGAGCTCGACCGGTTGGGCG
GATTCTTCATGGCGCTCACGGGCGCGGTAGCGGCTCCGGTCGGTTGTTACCTGATCGGCTACGTGCGCCGTGAACACCTC
GGTCGGGTCCCGATGGCGGTGGTGCCGCTGTTCGTCGCGGCGATGCTGTTGGTGCCGGCCGCGGGCTCGGTGACGACGTT
TCTGCTGGCGTGGGAGCTGATGGCGATCGCGTCGCTGATCCTGGTGCTCTCCGAGCACGCCCGCCCGCAGGTCCGCTCGG
CGGGCCTGTGGTACGCCGTGATGACTCAGCTGGGATTCATCGCAATCCTGGTCGGGCTGGTGGTGTTGGCGGCGGCCGGG
GGTTCCGACCGGTTCGCCGGCCTCGGGGCAGTCTGCGACGGGGTCCGCGCCGCCGTATTTATGCTCACGCTGGTCGGGTT
TGGTTCGAAGGCGGGCCTGGTGCCACTGCACGCCTGGCTGCCGCGGGCCCACCCGGAGGCGCCGAGCCCGGTGTCGGCGT
TGATGAGCGCGGCGATGGTCAACCTGGGCATCTACGGCATCGTCCGTTTCGATCTGCAGCTGCTGGGGCCGGGCCCACGC
TGGTGGGGGCTTGCGCTGCTGGCCGTGGGCGGCACGTCCGCGCTGTATGGGGTGCTGCAGGCTTCGGTGGCCGCCGATCT
CAAACGGCTGCTGGCCTATTCGACGACCGAGAACATGGGCCTGATCACGCTGGCGCTCGGTGCGGCAACACTTTTCGCGG
ATACCGGAGCCTACGGGCCGGCGTCGATCGCCGCCGCCGCAGCGATGCTGCACATGATTGCGCACGCGGCGTTTAAGAGC
CTCGCCTTCATGGCGGCCGGATCTGTGCTGGCCGCGACCGGGCTGCGCGACCTGGACCTGCTCGGCGGGCTGGCCCGCCG
AATGCCGGCGACCACCGTCTTTTTCGGGGTGGCCGCACTGGGCGCATGTGGTCTGCCGTTGGGCGCCGGGTTTGTCAGTG
AGTGGCTGCTGGTCCAGTCGTTGATCCACGCTGCCCCCGGACACGACCCCATCGTGGCGCTGACGACACCGCTGGCGGTC
GGCGTGGTCGCACTGGCCACCGGTCTGAGCGTGGCGGCGATGACCAAGGCCTTCGGGATCGGGTTTCTCGCCCGTCCCCG
CTCCACCCAAGCCGAAGCGGCGCGTGAGGCGCCGGCCAGCATGCGCGCCGGCATGGCGATCGCGGCGGGCGCCTGCCTGG
TGCTGGCGGTGGCACCGCTGCTGGTCGCACCCATGGTGCGGCGGGCCGCCGCGACGCTGCCGGCCGCTCAGGCGGTCAAG
TTCACCGGTCTGGGCGCCGTGGTGCGGCTGCCCGCGATGTCCGGGTCGATCGCGCCCGGCGTGATCGCCGCCGCTGTGCT
CGCCGCGGCGTTGGCGGTAGCCGTCCTCGCGCGGTGGCGTTTCCGCCGGCGCCCGGCGCCGGCCAGGTTGCCGCTGTGGG
CTTGCGGCGCGGCCGATCTCACCGTGCGCATGCAATACACGGCCACGTCGTTCGCCGAGCCGCTGCAGCGGGTCTTCGGC
GACGTGCTGCGCCCGGACACCGACATCGAGGTCACCCACACCGCCGAGTCGCGCTATATGGCCGAGCGGATCACCTACCG
GACCGCGGTCGCCGACGCGATCGAACAGCGCCTCTATACGCCGGTGGTCGGGGCGGTGGCCGCCATGGCCGAGCTGCTGC
GCCGTGCCCACACCGGCAGCGTGCACCGCTACCTGGCCTACGGCGCGCTGGGCGTACTGATCGTGCTGGTGGTCGCGAGG
TGTACGTGA

Upstream 100 bases:

>100_bases
TCGGTGCGGTCGGCGAGGTGGTACCCGTCGACGTCGAGATCGCCGGCTGCCCGCCGACACCCGCGGCCATCATGGCGGCG
CTGCGATCGGTGACCGGGAA

Downstream 100 bases:

>100_bases
TGTCCTACCTAGCGGGCGCCGCGCAAATCGGCGGGGTCATGGTGGGTGCGCCGCTGGTCATCGGTATGACGCGGCAGGTA
CGGGCACGCTGGGAAGGCCG

Product: putative oxidoreductase

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MTAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALTGAVAAPVGCYLIGYVRREHL
GRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLILVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAG
GSDRFAGLGAVCDGVRAAVFMLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGPASIAAAAAMLHMIAHAAFKS
LAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAALGACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAV
GVVALATGLSVAAMTKAFGIGFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADLTVRMQYTATSFAEPLQRVFG
DVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYTPVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVAR
CT

Sequences:

>Translated_642_residues
MTAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALTGAVAAPVGCYLIGYVRREHL
GRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLILVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAG
GSDRFAGLGAVCDGVRAAVFMLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGPASIAAAAAMLHMIAHAAFKS
LAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAALGACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAV
GVVALATGLSVAAMTKAFGIGFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADLTVRMQYTATSFAEPLQRVFG
DVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYTPVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVAR
CT
>Mature_641_residues
TAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALTGAVAAPVGCYLIGYVRREHLG
RVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLILVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAGG
SDRFAGLGAVCDGVRAAVFMLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPRW
WGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGPASIAAAAAMLHMIAHAAFKSL
AFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAALGACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAVG
VVALATGLSVAAMTKAFGIGFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVKF
TGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADLTVRMQYTATSFAEPLQRVFGD
VLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYTPVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVARC
T

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=466, Percent_Identity=24.6781115879828, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI251831116, Length=289, Percent_Identity=25.9515570934256, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1788827, Length=636, Percent_Identity=30.9748427672956, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI2367154, Length=529, Percent_Identity=29.8676748582231, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788831, Length=358, Percent_Identity=33.7988826815642, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788614, Length=343, Percent_Identity=30.6122448979592, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788613, Length=219, Percent_Identity=28.7671232876712, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788829, Length=335, Percent_Identity=27.4626865671642, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 65833; Mature: 65702

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALT
CCCCCCCCCCHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEHHHHCHHHHHHH
GAVAAPVGCYLIGYVRREHLGRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAGGSDRFAGLGAVCDGVRAAVF
HHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
HHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEHHEECCCCH
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCH
ASIAAAAAMLHMIAHAAFKSLAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
GACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAVGVVALATGLSVAAMTKAFGI
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
GFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
CCCCCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHE
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADL
ECCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCE
TVRMQYTATSFAEPLQRVFGDVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYT
EEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
PVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVARCT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALT
CCCCCCCCCHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEHHHHCHHHHHHH
GAVAAPVGCYLIGYVRREHLGRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAGGSDRFAGLGAVCDGVRAAVF
HHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
HHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEHHEECCCCH
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCH
ASIAAAAAMLHMIAHAAFKSLAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
GACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAVGVVALATGLSVAAMTKAFGI
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
GFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
CCCCCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHE
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADL
ECCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCE
TVRMQYTATSFAEPLQRVFGDVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYT
EEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
PVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVARCT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]