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The map label for this gene is ponA1 [H]

Identifier: 121635960

GI number: 121635960

Start: 83313

End: 85358

Strand: Direct

Name: ponA1 [H]

Synonym: BCG_0081

Alternate gene names: 121635960

Gene position: 83313-85358 (Clockwise)

Preceding gene: 121635959

Following gene: 121635961

Centisome position: 1.9

GC content: 63.39

Gene sequence:

>2046_bases
GTGGTGATCCTGTTGCTGATGGTCACCTTCACGATGGCCTACCTGATCGTCGACGTTCCCAAGCCAGGTGACATCCGTAC
CAACCAGGTCTCCACGATCCTTGCCAGCGACGGCTCGGAAATCGCCAAAATTGTTCCGCCCGAAGGTAATCGGGTCGACG
TCAACCTCAGCCAGGTGCCGATGCATGTGCGCCAGGCGGTGATTGCGGCCGAAGACCGCAATTTCTATTCGAATCCGGGA
TTCTCGTTCACCGGCTTCGCGCGGGCAGTCAAGAACAACCTGTTCGGCGGCGATCTGCAGGGCGGATCGACGATTACCCA
GCAGTACGTCAAGAACGCGCTGGTCGGTTCCGCACAGCACGGGTGGAGCGGTCTGATGCGCAAGGCGAAAGAATTGGTCA
TCGCGACGAAGATGTCGGGGGAGTGGTCTAAAGACGATGTGCTGCAGGCGTATCTGAACATCATCTACTTCGGCCGGGGC
GCCTACGGCATTTCGGCGGCGTCCAAGGCTTATTTCGACAAGCCCGTCGAGCAGCTGACCGTTGCCGAAGGGGCGTTGTT
GGCAGCGCTGATTCGGCGGCCTTCGACGCTGGACCCGGCGGTCGACCCCGAAGGGGCCCATGCCCGCTGGAATTGGGTAC
TCGACGGCATGGTGGAAACCAAGGCTCTCTCGCCGAATGACCGTGCGGCGCAGGTGTTTCCCGAGACAGTGCCGCCCGAT
CTGGCCCGGGCGGAGAATCAGACCAAAGGACCCAACGGGCTGATCGAGCGGCAGGTGACAAGGGAGTTGCTCGAGCTGTT
CAACATCGACGAGCAGACCCTCAACACCCAGGGGCTGGTGGTCACCACCACGATTGATCCGCAGGCCCAACGGGCGGCGG
AGAAGGCGGTTGCGAAATACCTGGACGGGCAGGACCCCGACATGCGTGCCGCCGTGGTTTCCATCGACCCGCACAACGGG
GCGGTGCGTGCGTACTACGGTGGCGACAATGCCAATGGCTTTGACTTCGCTCAAGCGGGATTGCAGACTGGATCGTCGTT
TAAGGTGTTTGCTCTGGTGGCCGCCCTTGAGCAGGGGATCGGCCTGGGCTACCAGGTAGACAGCTCTCCGTTGACGGTCG
ACGGCATCAAGATCACCAACGTCGAGGGCGAGGGTTGCGGGACGTGCAACATCGCCGAGGCGCTCAAAATGTCGCTGAAC
ACCTCCTACTACCGGCTGATGCTCAAGCTCAACGGCGGCCCACAGGCTGTGGCCGATGCCGCGCACCAAGCCGGCATTGC
CTCCAGCTTCCCGGGCGTTGCGCACACGCTGTCCGAAGATGGCAAGGGTGGACCGCCCAACAACGGGATCGTGTTGGGCC
AGTACCAAACCCGGGTGATCGACATGGCATCGGCGTATGCCACGTTGGCCGCGTCCGGTATCTACCACCCGCCGCATTTC
GTACAGAAGGTGGTCAGTGCCAACGGCCAGGTCCTCTTCGACGCCAGCACCGCGGACAACACCGGCGATCAGCGCATCCC
CAAGGCGGTAGCCGACAACGTGACTGCGGCGATGGAGCCGATCGCAGGTTATTCGCGTGGCCACAACCTAGCGGGTGGGC
GGGATTCGGCGGCCAAGACCGGCACTACGCAATTTGGTGACACCACCGCGAACAAAGACGCCTGGATGGTCGGGTACACG
CCGTCGTTGTCTACGGCTGTGTGGGTGGGCACCGTCAAGGGTGACGAGCCACTGGTAACCGCTTCGGGTGCAGCGATTTA
CGGCTCGGGCCTGCCGTCGGACATCTGGAAGGCAACCATGGACGGCGCCTTGAAGGGCACGTCGAACGAGACTTTCCCCA
AACCGACCGAGGTCGGTGGTTATGCCGGTGTGCCGCCGCCGCCGCCTCCGCCGCCGCCGTCGGAGGTACCACCTTCGGAG
ACCGTCATCCAGCCCACGGTCGAAATTGCGCCGGGGATTACCATCCCGATCGGTCCCCCGACCACCATTACCCTGGCGCC
ACCGCCCCCGGCCCCGCCCGCTGCGACTCCCACGCCGCCGCCGTGA

Upstream 100 bases:

>100_bases
ACAGCCTGCCGGCCGGCTGCCCCAACCGAGGGTGGACTTGCCCCGGGTCGGCCAGATCAACTGGAAATGGATACGGCGTT
CGCTGTACCTCACCGCGGCG

Downstream 100 bases:

>100_bases
CCGGCGCGCTGTCCCAAAGCAGCAACATCTCGCCACTTCCTTTGGCCGCCGATCTGCGGAGCGCCGATAACCGCGATTGC
CCCAGCCGCACCGACGTATT

Product: putative bifunctional penicillin-binding protein 1A/1B PONA1

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MVILLLMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVPMHVRQAVIAAEDRNFYSNPG
FSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQHGWSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRG
AYGISAASKAYFDKPVEQLTVAEGALLAALIRRPSTLDPAVDPEGAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPD
LARAENQTKGPNGLIERQVTRELLELFNIDEQTLNTQGLVVTTTIDPQAQRAAEKAVAKYLDGQDPDMRAAVVSIDPHNG
AVRAYYGGDNANGFDFAQAGLQTGSSFKVFALVAALEQGIGLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLN
TSYYRLMLKLNGGPQAVADAAHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAASGIYHPPHF
VQKVVSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSAAKTGTTQFGDTTANKDAWMVGYT
PSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATMDGALKGTSNETFPKPTEVGGYAGVPPPPPPPPPSEVPPSE
TVIQPTVEIAPGITIPIGPPTTITLAPPPPAPPAATPTPPP

Sequences:

>Translated_681_residues
MVILLLMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVPMHVRQAVIAAEDRNFYSNPG
FSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQHGWSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRG
AYGISAASKAYFDKPVEQLTVAEGALLAALIRRPSTLDPAVDPEGAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPD
LARAENQTKGPNGLIERQVTRELLELFNIDEQTLNTQGLVVTTTIDPQAQRAAEKAVAKYLDGQDPDMRAAVVSIDPHNG
AVRAYYGGDNANGFDFAQAGLQTGSSFKVFALVAALEQGIGLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLN
TSYYRLMLKLNGGPQAVADAAHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAASGIYHPPHF
VQKVVSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSAAKTGTTQFGDTTANKDAWMVGYT
PSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATMDGALKGTSNETFPKPTEVGGYAGVPPPPPPPPPSEVPPSE
TVIQPTVEIAPGITIPIGPPTTITLAPPPPAPPAATPTPPP
>Mature_681_residues
MVILLLMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVPMHVRQAVIAAEDRNFYSNPG
FSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQHGWSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRG
AYGISAASKAYFDKPVEQLTVAEGALLAALIRRPSTLDPAVDPEGAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPD
LARAENQTKGPNGLIERQVTRELLELFNIDEQTLNTQGLVVTTTIDPQAQRAAEKAVAKYLDGQDPDMRAAVVSIDPHNG
AVRAYYGGDNANGFDFAQAGLQTGSSFKVFALVAALEQGIGLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLN
TSYYRLMLKLNGGPQAVADAAHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAASGIYHPPHF
VQKVVSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSAAKTGTTQFGDTTANKDAWMVGYT
PSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATMDGALKGTSNETFPKPTEVGGYAGVPPPPPPPPPSEVPPSE
TVIQPTVEIAPGITIPIGPPTTITLAPPPPAPPAATPTPPP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=631, Percent_Identity=27.5752773375594, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI87082258, Length=293, Percent_Identity=34.4709897610922, Blast_Score=139, Evalue=8e-34,
Organism=Escherichia coli, GI1788867, Length=575, Percent_Identity=25.3913043478261, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=34.3195266272189, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 71448; Mature: 71448

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVILLLMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVP
CCHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCEEEEECCCCCEEEEEHHHHH
MHVRQAVIAAEDRNFYSNPGFSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQH
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GWSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRGAYGISAASKAYFDKPVEQLT
CHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCHHHHHH
VAEGALLAALIRRPSTLDPAVDPEGAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPD
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEHHCCCCCCCCHHHHCCCCCCHH
LARAENQTKGPNGLIERQVTRELLELFNIDEQTLNTQGLVVTTTIDPQAQRAAEKAVAKY
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
LDGQDPDMRAAVVSIDPHNGAVRAYYGGDNANGFDFAQAGLQTGSSFKVFALVAALEQGI
HCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
GLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLNTSYYRLMLKLNGGPQAVADA
CCCEEECCCCEEECCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
AHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAASGIYHPPHF
HHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCHHH
VQKVVSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSAAKT
HHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GTTQFGDTTANKDAWMVGYTPSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATM
CCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEECCCEEECCCCCHHHHHHHH
DGALKGTSNETFPKPTEVGGYAGVPPPPPPPPPSEVPPSETVIQPTVEIAPGITIPIGPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCCCEEEECCCC
TTITLAPPPPAPPAATPTPPP
CEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVILLLMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVP
CCHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCEEEEECCCCCEEEEEHHHHH
MHVRQAVIAAEDRNFYSNPGFSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQH
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
GWSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRGAYGISAASKAYFDKPVEQLT
CHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCHHHHHH
VAEGALLAALIRRPSTLDPAVDPEGAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPD
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEHHCCCCCCCCHHHHCCCCCCHH
LARAENQTKGPNGLIERQVTRELLELFNIDEQTLNTQGLVVTTTIDPQAQRAAEKAVAKY
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
LDGQDPDMRAAVVSIDPHNGAVRAYYGGDNANGFDFAQAGLQTGSSFKVFALVAALEQGI
HCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
GLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLNTSYYRLMLKLNGGPQAVADA
CCCEEECCCCEEECCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCHHHHHH
AHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAASGIYHPPHF
HHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCHHH
VQKVVSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSAAKT
HHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GTTQFGDTTANKDAWMVGYTPSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATM
CCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEECCCEEECCCCCHHHHHHHH
DGALKGTSNETFPKPTEVGGYAGVPPPPPPPPPSEVPPSETVIQPTVEIAPGITIPIGPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCCCEEEECCCC
TTITLAPPPPAPPAATPTPPP
CEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]