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The map label for this gene is recF

Identifier: 121635913

GI number: 121635913

Start: 32947

End: 34104

Strand: Direct

Name: recF

Synonym: BCG_0033

Alternate gene names: 121635913

Gene position: 32947-34104 (Clockwise)

Preceding gene: 121635912

Following gene: 121635914

Centisome position: 0.75

GC content: 60.1

Gene sequence:

>1158_bases
GTGTACGTCCGTCATTTGGGGCTGCGTGACTTCCGGTCCTGGGCATGTGTAGATCTGGAATTGCATCCAGGGCGGACGGT
TTTTGTTGGGCCTAACGGTTATGGTAAGACGAATCTTATTGAGGCACTGTGGTATTCGACGACGTTAGGTTCGCACCGCG
TTAGCGCCGATTTGCCGTTGATCCGGGTAGGTACCGATCGTGCGGTGATCTCCACGATCGTGGTGAACGACGGTAGAGAA
TGTGCCGTCGACCTCGAGATCGCCACGGGGCGAGTCAACAAAGCGCGATTGAATCGATCATCGGTCCGAAGTACACGTGA
TGTGGTCGGAGTGCTTCGAGCTGTGTTGTTTGCCCCTGAGGATCTGGGGTTGGTTCGTGGGGATCCCGCTGACCGGCGGC
GCTATCTGGATGATCTGGCGATCGTGCGTAGGCCTGCGATCGCTGCGGTACGAGCCGAATATGAGAGGGTGGTGCGCCAG
CGGACGGCGTTATTGAAGTCCGTACCTGGAGCACGGTATCGGGGTGACCGGGGTGTGTTTGACACTCTTGAGGTATGGGA
CAGTCGTTTGGCGGAGCACGGGGCTGAACTGGTGGCCGCCCGCATCGATTTGGTCAACCAGTTGGCACCGGAAGTGAAGA
AGGCATACCAGCTGTTGGCGCCGGAATCGCGATCGGCGTCTATCGGTTATCGGGCCAGCATGGATGTAACCGGTCCCAGC
GAGCAGTCAGATACCGATCGGCAATTGTTAGCAGCTCGGCTGTTGGCGGCGCTGGCGGCCCGTCGGGATGCCGAACTCGA
GCGTGGGGTTTGTCTAGTTGGTCCGCACCGTGACGACCTAATACTGCGACTAGGCGATCAACCCGCGAAAGGATTTGCTA
GCCATGGGGAGGCGTGGTCGTTGGCGGTGGCACTGCGGTTGGCGGCCTATCAACTGTTACGCGTTGATGGTGGTGAGCCG
GTGTTGTTGCTCGACGACGTGTTCGCCGAACTGGATGTCATGCGCCGTCGAGCGTTGGCGACGGCGGCCGAGTCCGCCGA
ACAGGTGTTGGTGACTGCCGCGGTGCTCGAGGATATTCCCGCCGGCTGGGACGCCAGGCGGGTGCACATCGATGTGCGTG
CCGATGACACCGGATCGATGTCGGTGGTTCTGCCATGA

Upstream 100 bases:

>100_bases
GGTCTGAATGGCAACGGTCCGTTCCCGGCGGTGTCGACGGACTATGTCTATCTGTTGATGCCGGTTCGGTTGCCGGGCTG
AGCACTTGGCGCCCGGGTAG

Downstream 100 bases:

>100_bases
CGGGTTCTGTTGACCGGCCCGACCAGAATCGCGGTGAGCGATCAATGAAGTCACCAGGGTTGGATTTGGTCAGGCGCACC
CTGGACGAAGCTCGTGCTGC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPLIRVGTDRAVISTIVVNDGRE
CAVDLEIATGRVNKARLNRSSVRSTRDVVGVLRAVLFAPEDLGLVRGDPADRRRYLDDLAIVRRPAIAAVRAEYERVVRQ
RTALLKSVPGARYRGDRGVFDTLEVWDSRLAEHGAELVAARIDLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPS
EQSDTDRQLLAARLLAALAARRDAELERGVCLVGPHRDDLILRLGDQPAKGFASHGEAWSLAVALRLAAYQLLRVDGGEP
VLLLDDVFAELDVMRRRALATAAESAEQVLVTAAVLEDIPAGWDARRVHIDVRADDTGSMSVVLP

Sequences:

>Translated_385_residues
MYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPLIRVGTDRAVISTIVVNDGRE
CAVDLEIATGRVNKARLNRSSVRSTRDVVGVLRAVLFAPEDLGLVRGDPADRRRYLDDLAIVRRPAIAAVRAEYERVVRQ
RTALLKSVPGARYRGDRGVFDTLEVWDSRLAEHGAELVAARIDLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPS
EQSDTDRQLLAARLLAALAARRDAELERGVCLVGPHRDDLILRLGDQPAKGFASHGEAWSLAVALRLAAYQLLRVDGGEP
VLLLDDVFAELDVMRRRALATAAESAEQVLVTAAVLEDIPAGWDARRVHIDVRADDTGSMSVVLP
>Mature_385_residues
MYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPLIRVGTDRAVISTIVVNDGRE
CAVDLEIATGRVNKARLNRSSVRSTRDVVGVLRAVLFAPEDLGLVRGDPADRRRYLDDLAIVRRPAIAAVRAEYERVVRQ
RTALLKSVPGARYRGDRGVFDTLEVWDSRLAEHGAELVAARIDLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPS
EQSDTDRQLLAARLLAALAARRDAELERGVCLVGPHRDDLILRLGDQPAKGFASHGEAWSLAVALRLAAYQLLRVDGGEP
VLLLDDVFAELDVMRRRALATAAESAEQVLVTAAVLEDIPAGWDARRVHIDVRADDTGSMSVVLP

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=376, Percent_Identity=27.9255319148936, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_MYCBO (Q7U314)

Other databases:

- EMBL:   BX248334
- RefSeq:   NP_853673.1
- ProteinModelPortal:   Q7U314
- SMR:   Q7U314
- EnsemblBacteria:   EBMYCT00000015312
- GeneID:   1090745
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0003
- GeneTree:   EBGT00050000017189
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   MBOV233413:MB0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42187; Mature: 42187

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPL
CCEECCCCHHCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCE
IRVGTDRAVISTIVVNDGRECAVDLEIATGRVNKARLNRSSVRSTRDVVGVLRAVLFAPE
EEECCCHHEEEEEEECCCCEEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DLGLVRGDPADRRRYLDDLAIVRRPAIAAVRAEYERVVRQRTALLKSVPGARYRGDRGVF
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DTLEVWDSRLAEHGAELVAARIDLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
EQSDTDRQLLAARLLAALAARRDAELERGVCLVGPHRDDLILRLGDQPAKGFASHGEAWS
CCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCEEEEECCCCCCCHHCCCCHHH
LAVALRLAAYQLLRVDGGEPVLLLDDVFAELDVMRRRALATAAESAEQVLVTAAVLEDIP
HHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGWDARRVHIDVRADDTGSMSVVLP
CCCCCEEEEEEEEECCCCCEEEEEC
>Mature Secondary Structure
MYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPL
CCEECCCCHHCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCE
IRVGTDRAVISTIVVNDGRECAVDLEIATGRVNKARLNRSSVRSTRDVVGVLRAVLFAPE
EEECCCHHEEEEEEECCCCEEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DLGLVRGDPADRRRYLDDLAIVRRPAIAAVRAEYERVVRQRTALLKSVPGARYRGDRGVF
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DTLEVWDSRLAEHGAELVAARIDLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCC
EQSDTDRQLLAARLLAALAARRDAELERGVCLVGPHRDDLILRLGDQPAKGFASHGEAWS
CCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCEEEEECCCCCCCHHCCCCHHH
LAVALRLAAYQLLRVDGGEPVLLLDDVFAELDVMRRRALATAAESAEQVLVTAAVLEDIP
HHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AGWDARRVHIDVRADDTGSMSVVLP
CCCCCEEEEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972